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. 1996 Oct;70(10):7224–7227. doi: 10.1128/jvi.70.10.7224-7227.1996

Differential interaction of temperature-sensitive simian virus 40 T antigens with tumor suppressors pRb and p53.

S Ray 1, M E Anderson 1, P Tegtmeyer 1
PMCID: PMC190777  PMID: 8794371

Abstract

Previous studies have shown that simian virus 40 large T antigen transforms cells by binding and inactivating suppressors of cell cycle progression and tumor formation. Here, we characterize the interactions of five temperature-sensitive T antigens with the tumor suppressor proteins pRb and p53. All five mutant T antigens bind pRb at the nonpermissive temperature with efficiencies similar to that of wild-type T antigen. A single transformation-competent mutant, with a substitution of amino acid 186, binds p53 at the nonpermissive temperature. Four transformation-defective mutants, with a substitution at T antigen position 357, 422, 427, or 438, are temperature sensitive for the binding and inactivation of p53. Our findings provide a basis for understanding the behavior of cells transformed by temperature-sensitive T antigens.

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Selected References

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