Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1992 Nov;107(3):790–796. doi: 10.1111/j.1476-5381.1992.tb14525.x

Membrane potential and current responses to neurotensin in the longitudinal muscle of the rectum of the fowl.

S Komori 1, T Matsuoka 1, S C Kwon 1, T Takewaki 1, H Ohashi 1
PMCID: PMC1907771  PMID: 1472975

Abstract

1. The effects of neurotensin (NT) on membrane potential and membrane current of the longitudinal smooth muscle of chicken rectum were investigated by intracellular recording and whole-cell voltage clamp. 2. NT (3 nM-1.2 microM), when applied via the bathing medium, produced a concentration-dependent membrane depolarization with an EC50 of 18 +/- 2 nM (n = 7) which was accompanied by an increase in the membrane conductance. The effect was biphasic: an initial, rapid depolarization reached a peak within 2-3 min and then declined to a lower but still elevated level which was sustained until washout. 3. Excitatory junction potentials (e.j.ps), which were non-adrenergic non-cholinergic (NANC) in nature, were decreased in amplitude and total duration in the presence of NT (0.6 microM). The depression of the e.j.p. was due mainly to the reduction of the membrane resistance. 4. When NT was applied locally by means of pressure ejection from a micropipette containing NT, some cells responded with a membrane depolarization and some failed to respond, whereas e.j.ps could invariably be elicited from all of them. 5. In single muscle cells enzymatically isolated from the muscle and dialyzed under voltage clamp at -50 mV with a CsCl-rich solution, NT (5 or 10 microM) produced an inward current. NT-induced inward currents were obtained with inclusion of 10 mM EGTA in the pipette solution and their reversal potential was around 0 mV. In cells dialyzed under voltage clamp at 0 mV with a KCl-rich solution, NT (5 microM) produced a brief outward current followed by abolition of spontaneous transient outward currents.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
790

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe Y., Tomita T. Cable properties of smooth muscle. J Physiol. 1968 May;196(1):87–100. doi: 10.1113/jphysiol.1968.sp008496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benham C. D., Bolton T. B. Spontaneous transient outward currents in single visceral and vascular smooth muscle cells of the rabbit. J Physiol. 1986 Dec;381:385–406. doi: 10.1113/jphysiol.1986.sp016333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carraway R., Bhatnagar Y. M. Immunochemical characterization of neurotensin-like peptides in chicken. Peptides. 1980 Summer;1(2):159–165. doi: 10.1016/0196-9781(80)90081-9. [DOI] [PubMed] [Google Scholar]
  4. Carraway R., Bhatnagar Y. M. Isolation, structure and biologic activity of chicken intestinal neurotensin. Peptides. 1980 Summer;1(2):167–174. doi: 10.1016/0196-9781(80)90082-0. [DOI] [PubMed] [Google Scholar]
  5. Carraway R., Leeman S. E. The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalami. J Biol Chem. 1973 Oct 10;248(19):6854–6861. [PubMed] [Google Scholar]
  6. Donoso M. V., Huidobro-Toro J. P., Kullak A. Involvement of calcium channels in the contractile activity of neurotensin but not acetylcholine: studies with calcium channel blockers and Bay K 8644 on the rat fundus. Br J Pharmacol. 1986 Aug;88(4):837–846. doi: 10.1111/j.1476-5381.1986.tb16257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iwabuchi H., Komori S., Ohashi H., Kimura S. The amino acid sequence of a smooth muscle-contracting peptide from chicken rectum. Identity to chicken neurotensin. Jpn J Pharmacol. 1987 Aug;44(4):455–459. doi: 10.1254/jjp.44.455. [DOI] [PubMed] [Google Scholar]
  8. Kitabgi P. Effects of neurotensin on intestinal smooth muscle: application to the study of structure-activity relationships. Ann N Y Acad Sci. 1982;400:37–55. doi: 10.1111/j.1749-6632.1982.tb31559.x. [DOI] [PubMed] [Google Scholar]
  9. Kitabgi P., Freychet P. Effects of neurotensin on isolated intestinal smooth muscles. Eur J Pharmacol. 1978 Aug 15;50(4):349–357. doi: 10.1016/0014-2999(78)90140-1. [DOI] [PubMed] [Google Scholar]
  10. Kitabgi P., Freychet P. Neurotensin contracts the guinea-pig longitudinal ileal smooth muscle by inducing acetylcholine release. Eur J Pharmacol. 1979 Jul 1;56(4):403–406. doi: 10.1016/0014-2999(79)90272-3. [DOI] [PubMed] [Google Scholar]
  11. Kitabgi P., Hamon G., Worcel M. Electrophysiological study of the action of neurotensin on the smooth muscle of the guinea-pig taenia coli. Eur J Pharmacol. 1979 Jun;56(1-2):87–93. doi: 10.1016/0014-2999(79)90437-0. [DOI] [PubMed] [Google Scholar]
  12. Kitabgi P., Vincent J. P. Neurotensin is a potent inhibitor of guinea-pig colon contractile activity. Eur J Pharmacol. 1981 Sep 24;74(4):311–318. doi: 10.1016/0014-2999(81)90050-9. [DOI] [PubMed] [Google Scholar]
  13. Komori S., Bolton T. B. Role of G-proteins in muscarinic receptor inward and outward currents in rabbit jejunal smooth muscle. J Physiol. 1990 Aug;427:395–419. doi: 10.1113/jphysiol.1990.sp018178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Komori S., Fukutome T., Ohashi H. Isolation of a peptide material showing strong rectal muscle-contracting activity from chicken rectum and its identification as chicken neurotensin. Jpn J Pharmacol. 1986 Apr;40(4):577–589. doi: 10.1254/jjp.40.577. [DOI] [PubMed] [Google Scholar]
  15. Komori S., Kawai M., Takewaki T., Ohashi H. GTP-binding protein involvement in membrane currents evoked by carbachol and histamine in guinea-pig ileal muscle. J Physiol. 1992 May;450:105–126. doi: 10.1113/jphysiol.1992.sp019118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Komori S., Mizutani A., Amano Y., Kanamaru Y., Ohashi H. Isolation of smooth muscle excitatory substances from chicken rectum and their characterization. Jpn J Pharmacol. 1986 Jan;40(1):103–113. doi: 10.1254/jjp.40.103. [DOI] [PubMed] [Google Scholar]
  17. Komori S., Ohashi H. Membrane potential responses to ATP applied by pressure ejection in the longitudinal muscle of chicken rectum. Br J Pharmacol. 1988 Dec;95(4):1157–1164. doi: 10.1111/j.1476-5381.1988.tb11751.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Komori S., Ohashi H. Some characteristics of transmission from non-adrenergic, non-cholinergic excitatory nerves to the smooth muscle of the chicken. J Auton Nerv Syst. 1982 Sep;6(2):199–210. doi: 10.1016/0165-1838(82)90051-0. [DOI] [PubMed] [Google Scholar]
  19. Komori S., Ohashi H. Some membrane properties of the circular muscle of chicken rectum and its non-adrenergic non-cholinergic innervation. J Physiol. 1988 Jul;401:417–435. doi: 10.1113/jphysiol.1988.sp017170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Quirion R., Rioux F., Regoli D., St-Pierre S. Selective blockade of neurotensin-induced coronary vessel constriction in perfused rat hearts by a neurotensin analogue. Eur J Pharmacol. 1980 Feb 8;61(3):309–312. doi: 10.1016/0014-2999(80)90133-8. [DOI] [PubMed] [Google Scholar]
  21. Rökaeus A., Burcher E., Chang D., Folkers K., Rosell S. Actions of neurotensin and (Gln4)-neurotensin on isolated tissues. Acta Pharmacol Toxicol (Copenh) 1977 Aug;41(2):141–147. doi: 10.1111/j.1600-0773.1977.tb02134.x. [DOI] [PubMed] [Google Scholar]
  22. Takewaki T., Ohashi O. Non-cholinergic excitatory transmission to intestinal smooth muscle cells. Nature. 1977 Aug 25;268(5622):749–750. doi: 10.1038/268749a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES