Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Jul;103(3):1657–1662. doi: 10.1111/j.1476-5381.1991.tb09843.x

The effect of streptozotocin-induced diabetes on cholinergic motor transmission in the rat urinary bladder.

G N Luheshi 1, M A Zar 1
PMCID: PMC1907794  PMID: 1657263

Abstract

1. The effect of streptozotocin (STZ)-induced diabetes on cholinergic motor transmission in the rat urinary bladder was investigated by recording contractile activity of detrusor strips in vitro. 2. The Ca(2+)-channel antagonist, nifedipine, was found to be more effective in blocking the noncholinergic motor transmission than P2-purinoceptor desensitization by alpha,beta-methylene ATP. 3. The neurogenic contractile responses to electrical field stimulation in the presence of nifedipine (cholinergic) were larger in the diabetic detrusor than in the non-diabetic controls. The potentiation of the cholinergic transmission was more evident at higher frequencies. 4. Concentration-response curves for acetylcholine were identical in detrusors from diabetic and non-diabetic animals, thus excluding a postsynaptic supersensitivity to acetylcholine being responsible for the potentiation of cholinergic motor transmission. 5. It is concluded that the potentiation of cholinergic motor transmission is due to enhanced release of acetylcholine in diabetic detrusor. Possible reasons for this enhancement are discussed in relation to diabetes.

Full text

PDF
1657

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambache N., Zar M. A. Non-cholinergic transmission by post-ganglionic motor neurones in the mammalian bladder. J Physiol. 1970 Oct;210(3):761–783. doi: 10.1113/jphysiol.1970.sp009240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley W. E. Diagnosis of urinary bladder dysfunction in diabetes mellitus. Ann Intern Med. 1980 Feb;92(2 Pt 2):323–326. doi: 10.7326/0003-4819-92-2-323. [DOI] [PubMed] [Google Scholar]
  3. Buck A. C., McRae C. U., Chisholm G. D. The diabetic bladder. Proc R Soc Med. 1974 Jan;67(1):81–83. [PMC free article] [PubMed] [Google Scholar]
  4. Burnstock G., Dumsday B., Smythe A. Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972 Mar;44(3):451–461. doi: 10.1111/j.1476-5381.1972.tb07283.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Choo L. K., Mitchelson F. The effect of indomethacin and adenosine 5'-triphosphate on the excitatory innervation of the rate urinary bladder. Can J Physiol Pharmacol. 1980 Sep;58(9):1042–1048. doi: 10.1139/y80-157. [DOI] [PubMed] [Google Scholar]
  6. Ekström J., Uvelius B. Length-tension relations of smooth muscle from normal and denervated rat urinary bladders. Acta Physiol Scand. 1981 Aug;112(4):443–447. doi: 10.1111/j.1748-1716.1981.tb06842.x. [DOI] [PubMed] [Google Scholar]
  7. Ellenberg M. Development of urinary bladder dysfunction in diabetes mellitus. Ann Intern Med. 1980 Feb;92(2 Pt 2):321–323. doi: 10.7326/0003-4819-92-2-321. [DOI] [PubMed] [Google Scholar]
  8. Faerman I., Maler M., Jadzinsky M., Alvarez E., Fox D., Zilbervarg J., Cibeira J. B., Colinas R. Asymptomatic neurogenic bladder in juvenile diabetics. Diabetologia. 1971 Jun;7(3):168–172. doi: 10.1007/BF01212549. [DOI] [PubMed] [Google Scholar]
  9. Goss R. J., Liang M. D., Weisholtz S. J., Peltzer T. J. The physiological basis of urinary bladder hypertrophy. Proc Soc Exp Biol Med. 1973 Apr;142(4):1332–1335. doi: 10.3181/00379727-142-37235. [DOI] [PubMed] [Google Scholar]
  10. Hoyle C. H., Chapple C., Burnstock G. Isolated human bladder: evidence for an adenine dinucleotide acting on P2X-purinoceptors and for purinergic transmission. Eur J Pharmacol. 1989 Dec 12;174(1):115–118. doi: 10.1016/0014-2999(89)90881-9. [DOI] [PubMed] [Google Scholar]
  11. Iacovou J. W., Hill S. J., Birmingham A. T. Agonist-induced contraction and accumulation of inositol phosphates in the guinea-pig detrusor: evidence that muscarinic and purinergic receptors raise intracellular calcium by different mechanisms. J Urol. 1990 Sep;144(3):775–779. doi: 10.1016/s0022-5347(17)39590-3. [DOI] [PubMed] [Google Scholar]
  12. Kahan M., Goldberg P. D., Mandel E. E. Neurogenic vesical dysfunction and diabetes mellitus. N Y State J Med. 1970 Oct 1;70(19):2448–2455. [PubMed] [Google Scholar]
  13. Kolta M. G., Wallace L. J., Gerald M. C. Streptozocin-induced diabetes affects rat urinary bladder response to autonomic agents. Diabetes. 1985 Sep;34(9):917–921. doi: 10.2337/diab.34.9.917. [DOI] [PubMed] [Google Scholar]
  14. Lincoln J., Crockett M., Haven A. J., Burnstock G. Rat bladder in the early stages of streptozotocin-induced diabetes: adrenergic and cholinergic innervation. Diabetologia. 1984 Jan;26(1):81–87. doi: 10.1007/BF00252269. [DOI] [PubMed] [Google Scholar]
  15. Longhurst P. A., Belis J. A. Abnormalities of rat bladder contractility in streptozotocin-induced diabetes mellitus. J Pharmacol Exp Ther. 1986 Sep;238(3):773–777. [PubMed] [Google Scholar]
  16. Luheshi G. N., Zar M. A. Inhibitory effect of streptozotocin-induced diabetes on non-cholinergic motor transmission in rat detrusor and its prevention by sorbinil. Br J Pharmacol. 1990 Oct;101(2):411–417. doi: 10.1111/j.1476-5381.1990.tb12723.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Luheshi G. N., Zar M. A. Presence of non-cholinergic motor transmission in human isolated bladder. J Pharm Pharmacol. 1990 Mar;42(3):223–224. doi: 10.1111/j.2042-7158.1990.tb05396.x. [DOI] [PubMed] [Google Scholar]
  18. Luheshi G., Zar A. Purinoceptor desensitization impairs but does not abolish the non-cholinergic motor transmission in rat isolated urinary bladder. Eur J Pharmacol. 1990 Aug 28;185(2-3):203–208. doi: 10.1016/0014-2999(90)90641-i. [DOI] [PubMed] [Google Scholar]
  19. Moss H. E., Lincoln J., Burnstock G. A study of bladder dysfunction during streptozotocin-induced diabetes in the rat using an in vitro whole bladder preparation. J Urol. 1987 Nov;138(5):1279–1284. doi: 10.1016/s0022-5347(17)43584-1. [DOI] [PubMed] [Google Scholar]
  20. Santicioli P., Gamse R., Maggi C. A., Meli A. Cystometric changes in the early phase of streptozotocin-induced diabetes in rats: evidence for sensory changes not correlated to diabetic neuropathy. Naunyn Schmiedebergs Arch Pharmacol. 1987 May;335(5):580–587. doi: 10.1007/BF00169128. [DOI] [PubMed] [Google Scholar]
  21. Sibley G. N. A comparison of spontaneous and nerve-mediated activity in bladder muscle from man, pig and rabbit. J Physiol. 1984 Sep;354:431–443. doi: 10.1113/jphysiol.1984.sp015386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steers W. D., Mackway A. M., Ciambotti J., de Groat W. C. Effects of streptozotocin-induced diabetes on bladder function in the rat. J Urol. 1990 May;143(5):1032–1036. doi: 10.1016/s0022-5347(17)40177-7. [DOI] [PubMed] [Google Scholar]
  23. Zar M. A., Iravani M. M., Luheshi G. N. Effect of nifedipine on the contractile responses of the isolated rat bladder. J Urol. 1990 Apr;143(4):835–839. doi: 10.1016/s0022-5347(17)40112-1. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES