Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1996 Oct;70(10):7247–7249. doi: 10.1128/jvi.70.10.7247-7249.1996

The structural proteins of infectious pancreatic virus are not glycosylated.

L Perez 1, P P Chiou 1, J C Leong 1
PMCID: PMC190782  PMID: 8794376

Abstract

The major capsid protein, VP2, of infectious pancreatic necrosis virus, a nonenveloped icosahedral virus, contains six N-glycosylation consensus sequences (Asn-X-[Thr/Ser]). Since VP2 contains the major virus-neutralizing epitopes, the possible role for glycosylation in capsid formation and antigenicity was examined. The carbohydrate content of the virion proteins was determined by chemical detection, pulse-chase experiments,[3H]mannose labeling, and alteration of protein migration on sodium dodecyl sulfate-polyacrylamide gels after tunicamycin treatment. No glycosylation of any virion protein was observed when the carbohydrate nature of the glycoprotein of infectious hematopoietic necrosis virus was detected.

Full Text

The Full Text of this article is available as a PDF (200.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caswell-Reno P., Reno P. W., Nicholson B. L. Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates. J Gen Virol. 1986 Oct;67(Pt 10):2193–2205. doi: 10.1099/0022-1317-67-10-2193. [DOI] [PubMed] [Google Scholar]
  2. Dobos P., Roberts T. E. The molecular biology of infectious pancreatic necrosis virus: a review. Can J Microbiol. 1983 Apr;29(4):377–384. doi: 10.1139/m83-062. [DOI] [PubMed] [Google Scholar]
  3. Dobos P., Rowe D. Peptide map comparison of infectious pancreatic necrosis virus-specific polypeptides. J Virol. 1977 Dec;24(3):805–820. doi: 10.1128/jvi.24.3.805-820.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dobos P. Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus. J Virol. 1977 Jan;21(1):242–258. doi: 10.1128/jvi.21.1.242-258.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duncan R., Mason C. L., Nagy E., Leong J. A., Dobos P. Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif. Virology. 1991 Apr;181(2):541–552. doi: 10.1016/0042-6822(91)90887-H. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elbein A. D. Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. Annu Rev Biochem. 1987;56:497–534. doi: 10.1146/annurev.bi.56.070187.002433. [DOI] [PubMed] [Google Scholar]
  7. Estay A., Farias G., Soler M., Kuznar J. Further analysis on the structural proteins of infectious pancreatic necrosis virus. Virus Res. 1990 Jan;15(1):85–95. doi: 10.1016/0168-1702(90)90015-4. [DOI] [PubMed] [Google Scholar]
  8. Fries E., Rothman J. E. Transport of vesicular stomatitis virus glycoprotein in a cell-free extract. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3870–3874. doi: 10.1073/pnas.77.7.3870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fryer J. L., Yusha A., Pilcher K. S. The in vitro cultivation of tissue and cells of Pacific salmon and steelhead trout. Ann N Y Acad Sci. 1965 Aug 10;126(1):566–586. doi: 10.1111/j.1749-6632.1965.tb14303.x. [DOI] [PubMed] [Google Scholar]
  10. Heine H. G., Hyatt A. D., Boyle D. B. Modification of infectious bursal disease virus antigen VP2 for cell surface location fails to enhance immunogenicity. Virus Res. 1994 Jun;32(3):313–328. doi: 10.1016/0168-1702(94)90080-9. [DOI] [PubMed] [Google Scholar]
  11. Huang M. T., Manning D. S., Warner M., Stephens E. B., Leong J. C. A physical map of the viral genome for infectious pancreatic necrosis virus Sp: analysis of cell-free translation products derived from viral cDNA clones. J Virol. 1986 Dec;60(3):1002–1011. doi: 10.1128/jvi.60.3.1002-1011.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leong J. A. Molecular and biotechnological approaches to fish vaccines. Curr Opin Biotechnol. 1993 Jun;4(3):286–293. doi: 10.1016/0958-1669(93)90097-g. [DOI] [PubMed] [Google Scholar]
  13. Manning D. S., Leong J. C. Expression in Escherichia coli of the large genomic segment of infectious pancreatic necrosis virus. Virology. 1990 Nov;179(1):16–25. doi: 10.1016/0042-6822(90)90268-v. [DOI] [PubMed] [Google Scholar]
  14. Manning D. S., Mason C. L., Leong J. C. Cell-free translational analysis of the processing of infectious pancreatic necrosis virus polyprotein. Virology. 1990 Nov;179(1):9–15. doi: 10.1016/0042-6822(90)90267-u. [DOI] [PubMed] [Google Scholar]
  15. Mertens P. P., Dobos P. Messenger RNA of infectious pancreatic necrosis virus is polycistronic. Nature. 1982 May 20;297(5863):243–246. doi: 10.1038/297243a0. [DOI] [PubMed] [Google Scholar]
  16. Tarrab E., Berthiaume L., Grothé S., O'Connor-McCourt M., Heppell J., Lecomte J. Evidence of a major neutralizable conformational epitope region on VP2 of infectious pancreatic necrosis virus. J Gen Virol. 1995 Mar;76(Pt 3):551–558. doi: 10.1099/0022-1317-76-3-551. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES