Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Jul;103(3):1776–1780. doi: 10.1111/j.1476-5381.1991.tb09862.x

The effects of naloxone on the cardiovascular and respiratory effects of centrally administered corticotrophin releasing factor in conscious rabbits.

C N May 1, C J Whitehead 1, C J Mathias 1
PMCID: PMC1907825  PMID: 1933140

Abstract

1. The effects of intracerebroventricular (i.c.v.) and intracisternal (i.c.) administration of corticotrophin releasing factor (CRF) (0.5 nmol kg-1) were examined in conscious rabbits. The effect of opioid receptor antagonism was examined to determine whether the responses to CRF were mediated by endogenous opioid peptides. 2. After i.c.v. CRF there was a rise in mean arterial pressure (MAP), heart rate (HR), plasma noradrenaline and adrenaline, and increased behavioural activity. Respiration rate increased, PaCO2 fell, but PaO2 was unchanged. 3. The pressor and behavioural effects of i.c.v. CRF were unaltered by high doses of intravenous naloxone (9 mumols kg-1 bolus followed by 9 mumols kg-1 min-1 infusion); these effects of CRF were also not prevented by double this dose of naloxone. Naloxone attenuated the CRF-induced tachycardia, blocked the increase in respiration rate and increased the fall in PaCO2. 4. After i.c. CRF (0.5 nmol kg-1) there were similar changes in MAP, HR, plasma catecholamines, respiration and behaviour. 5. These results indicate that in conscious rabbits the pressor effects of i.c.v. CRF are not mediated by endogenous opioid peptides. The finding that the effects of CRF were similar after i.c.v. and i.c. administration suggests that these responses may result from actions on brainstem rather than periventricular sites.

Full text

PDF
1776

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown M. R., Fisher L. A., Rivier J., Spiess J., Rivier C., Vale W. Corticotropin-releasing factor: effects on the sympathetic nervous system and oxygen consumption. Life Sci. 1982 Jan 11;30(2):207–210. doi: 10.1016/0024-3205(82)90654-3. [DOI] [PubMed] [Google Scholar]
  2. Burke S. L., Dorward P. K. Influence of endogenous opiates and cardiac afferents on renal nerve activity during haemorrhage in conscious rabbits. J Physiol. 1988 Aug;402:9–27. doi: 10.1113/jphysiol.1988.sp017191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chai S. Y., Tarjan E., McKinley M. J., Paxinos G., Mendelsohn F. A. Corticotropin-releasing factor receptors in the rabbit brain visualized by in vitro autoradiography. Brain Res. 1990 Mar 26;512(1):60–69. doi: 10.1016/0006-8993(90)91170-l. [DOI] [PubMed] [Google Scholar]
  4. Evans R. G., Ludbrook J., Van Leeuwen A. F. Role of central opiate receptor subtypes in the circulatory responses of awake rabbits to graded caval occlusions. J Physiol. 1989 Dec;419:15–31. doi: 10.1113/jphysiol.1989.sp017858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldberg W. The ventral surface of the brain stem: a scarcely explored region of pharmacological sensitivity. Neuroscience. 1976 Dec;1(6):427–441. doi: 10.1016/0306-4522(76)90093-2. [DOI] [PubMed] [Google Scholar]
  6. Fischman A. J., Moldow R. L. Distribution of CRF-like immunoreactivity in the rabbit. Peptides. 1982 Sep-Oct;3(5):841–843. doi: 10.1016/0196-9781(82)90025-0. [DOI] [PubMed] [Google Scholar]
  7. Fisher L. A., Brown M. R. Corticotropin-releasing factor and angiotensin II: comparison of CNS actions to influence neuroendocrine and cardiovascular function. Brain Res. 1984 Mar 26;296(1):41–47. doi: 10.1016/0006-8993(84)90509-2. [DOI] [PubMed] [Google Scholar]
  8. Fisher L. A., Jessen G., Brown M. R. Corticotropin-releasing factor (CRF): mechanism to elevate mean arterial pressure and heart rate. Regul Pept. 1983 Jan;5(2):153–161. doi: 10.1016/0167-0115(83)90123-4. [DOI] [PubMed] [Google Scholar]
  9. Fisher L. A., Rivier J., Rivier C., Spiess J., Vale W., Brown M. R. Corticotropin-releasing factor (CRF): central effects on mean arterial pressure and heart rate in rats. Endocrinology. 1982 Jun;110(6):2222–2224. doi: 10.1210/endo-110-6-2222. [DOI] [PubMed] [Google Scholar]
  10. Florentino A., Jiménez I., Naranjo J. R., Urdín M. C., Fuentes J. A. Hypotensive effect of naloxone on high blood pressure induced by stress in the rat. Life Sci. 1987 Nov 30;41(22):2445–2453. doi: 10.1016/0024-3205(87)90670-9. [DOI] [PubMed] [Google Scholar]
  11. May C. N., Dashwood M. R., Whitehead C. J., Mathias C. J. Differential cardiovascular and respiratory responses to central administration of selective opioid agonists in conscious rabbits: correlation with receptor distribution. Br J Pharmacol. 1989 Nov;98(3):903–913. doi: 10.1111/j.1476-5381.1989.tb14620.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. May C. N., Ham I. W., Heslop K. E., Stone F. A., Mathias C. J. Intravenous morphine causes hypertension, hyperglycaemia and increases sympatho-adrenal outflow in conscious rabbits. Clin Sci (Lond) 1988 Jul;75(1):71–77. doi: 10.1042/cs0750071. [DOI] [PubMed] [Google Scholar]
  13. May C. N., Whitehead C. J., Dashwood M. R., Mathias C. J. Investigation of the central sites at which morphine acts to cause hypertension in conscious rabbits. Br J Pharmacol. 1989 Jul;97(3):873–881. doi: 10.1111/j.1476-5381.1989.tb12027.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. May C. N., Whitehead C. J., Heslop K. E., Mathias C. J. Evidence that intravenous morphine stimulates central opiate receptors to increase sympatho-adrenal outflow and cause hypertension in conscious rabbits. Clin Sci (Lond) 1989 Apr;76(4):431–437. doi: 10.1042/cs0760431. [DOI] [PubMed] [Google Scholar]
  15. Merchenthaler I., Hynes M. A., Vigh S., Shally A. V., Petrusz P. Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat spinal cord. Brain Res. 1983 Sep 26;275(2):373–377. doi: 10.1016/0006-8993(83)91001-6. [DOI] [PubMed] [Google Scholar]
  16. Rutter P. C., Potocnik S. J., Ludbrook J. Factors influencing the effects of intravenous naloxone on arterial pressure and heart rate after haemorrhage in conscious rabbits. Clin Exp Pharmacol Physiol. 1986 May;13(5):383–397. doi: 10.1111/j.1440-1681.1986.tb00917.x. [DOI] [PubMed] [Google Scholar]
  17. Saunders W. S., Thornhill J. A. Pressor, tachycardic and behavioral excitatory responses in conscious rats following ICV administration of ACTH and CRF are blocked by naloxone pretreatment. Peptides. 1986 Jul-Aug;7(4):597–601. doi: 10.1016/0196-9781(86)90033-1. [DOI] [PubMed] [Google Scholar]
  18. Sawchenko P. E., Swanson L. W. Localization, colocalization, and plasticity of corticotropin-releasing factor immunoreactivity in rat brain. Fed Proc. 1985 Jan;44(1 Pt 2):221–227. [PubMed] [Google Scholar]
  19. Thompson R. C., Seasholtz A. F., Herbert E. Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression. Mol Endocrinol. 1987 May;1(5):363–370. doi: 10.1210/mend-1-5-363. [DOI] [PubMed] [Google Scholar]
  20. Vale W., Spiess J., Rivier C., Rivier J. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 1981 Sep 18;213(4514):1394–1397. doi: 10.1126/science.6267699. [DOI] [PubMed] [Google Scholar]
  21. WEST G. B. Liberation of adrenaline from the suprarenal gland of the rabbit. Br J Pharmacol Chemother. 1950 Dec;5(4):542–547. doi: 10.1111/j.1476-5381.1950.tb00606.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wynn P. C., Hauger R. L., Holmes M. C., Millan M. A., Catt K. J., Aguilera G. Brain and pituitary receptors for corticotropin releasing factor: localization and differential regulation after adrenalectomy. Peptides. 1984 Nov-Dec;5(6):1077–1084. doi: 10.1016/0196-9781(84)90174-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES