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. 1991 Aug;103(4):1923–1927. doi: 10.1111/j.1476-5381.1991.tb12353.x

Inhibitory effects of guanosine 3':5'-cyclic monophosphate on the synthesis of dopamine in the rat kidney.

P Soares-da-Silva 1, M H Fernandes 1
PMCID: PMC1908178  PMID: 1655148

Abstract

1. In the present study the effects of M&B 22,948, a guanosine 3':5'-cyclic monophosphate (cyclic GMP) selective phosphodiesterase inhibitor and of 8-bromo cyclic GMP were examined on the synthesis of dopamine from L-3,4-dihydroxyphenylalanine (L-DOPA) in rat cortical slices and in whole kidney homogenates. The deamination of newly-formed dopamine into 3,4-dihydroxyphenylacetic acid (DOPAC) was also studied. The assay of L-DOPA, dopamine, noradrenaline and DOPAC was performed by high performance liquid chromatography (h.p.l.c.) with electrochemical detection. 2. Incubation of renal slices and homogenates of whole kidney with exogenous L-DOPA (0.1-10.0 microM) resulted in a concentration-dependent formation of both dopamine and DOPAC. 3. The addition of M&B 22,948 (10 microM) to the incubation medium resulted in a marked reduction in the accumulation of both newly-formed dopamine and DOPAC in kidney slices; the inhibitory effect of M&B 22,948 on DOPAC formation was greater than that on dopamine. 8-Bromo cyclic GMP (250 microM) produced only a slight decrease in the tissue levels of newly-formed dopamine (5-13% reduction), but was found to decrease significantly (51-68% reduction) the formation of DOPAC in kidney slices. The addition of 8-bromo cyclic GMP plus M&B 22,948 to the incubation medium resulted in similar effects to those described for M&B 22,948 alone. 4. In kidney homogenates, in contrast to results observed in kidney slices, M&B 22,948 (10 microM) and 8-bromo cyclic GMP (250 microM) were found to affect neither the formation of dopamine nor its deamination to DOPAC.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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