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. 1991 Nov;104(3):609–612. doi: 10.1111/j.1476-5381.1991.tb12477.x

Response of rabbit ear artery to endothelin-1 during cooling.

L Monge 1, A L García-Villalón 1, J J Montoya 1, J L García 1, B Gómez 1, G Diéguez 1
PMCID: PMC1908233  PMID: 1797325

Abstract

1. The effects of cooling on the response of rabbit central ear artery to endothelin-1 and the role of the endothelium in these effects were studied in 2 mm long cylindrical arterial segments. 2. Concentration-response curves for endothelin-1 (10(-10)-3 x 10(-7) M) were recorded isometrically in arteries with and without endothelium at 37 degrees C and during cooling (24 degrees C). To analyze further the endothelial mechanisms of the response to endothelin-1 during cooling, the effects of this peptide in the presence of NG-nitro-L-arginine methyl ester (L-NAME) (10(-4) M) or meclofenamate (10(-5) M) were also determined. 3. In every condition tested, endothelin-1 produced a marked, concentration-dependent arterial contraction. Sensitivity of intact arteries to this peptide was consistently lower at 24 degrees C than at 37 degrees C. At 37 degrees C there were comparable responses of arteries with and without endothelium, but at 24 degrees C arteries without endothelium showed a higher sensitivity than intact arteries to endothelin-1. 4. L-NAME (10(-4) M) increased the maximal contraction at 37 degrees C, and both the sensitivity and maximal contraction at 24 degrees C of intact arteries to endothelin-1. Meclofenamate (10(-5) M) did not affect the arterial response to endothelin-1. 5. Sensitivity of arteries with and without endothelium to nitroprusside (10(-9)-10(-3) M) was significantly decreased during cooling, and endothelium removal did not affect the relaxation to this nitrovasodilator. 6. These results suggest that cooling decreases sensitivity of cutaneous arteries (ear artery) to endothelin-1 probably by increasing the availability of endothelial nitric oxide.

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Selected References

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  1. Bodelsson M., Arneklo-Nobin B., Törnebrandt K. Cooling augments contractile response to 5-hydroxytryptamine via an endothelium-dependent mechanism. Blood Vessels. 1989;26(6):347–359. doi: 10.1159/000158785. [DOI] [PubMed] [Google Scholar]
  2. Douglas S. A., Hiley C. R. Endothelium-dependent vascular activities of endothelin-like peptides in the isolated superior mesenteric arterial bed of the rat. Br J Pharmacol. 1990 Sep;101(1):81–88. doi: 10.1111/j.1476-5381.1990.tb12093.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eglen R. M., Michel A. D., Sharif N. A., Swank S. R., Whiting R. L. The pharmacological properties of the peptide, endothelin. Br J Pharmacol. 1989 Aug;97(4):1297–1307. doi: 10.1111/j.1476-5381.1989.tb12592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Furchgott R. F., Vanhoutte P. M. Endothelium-derived relaxing and contracting factors. FASEB J. 1989 Jul;3(9):2007–2018. [PubMed] [Google Scholar]
  5. Garcia J. L., Monge L., Gómez B., Diéguez G. Response of canine cerebral arteries to endothelin-1. J Pharm Pharmacol. 1991 Apr;43(4):281–284. doi: 10.1111/j.2042-7158.1991.tb06686.x. [DOI] [PubMed] [Google Scholar]
  6. Gardiner S. M., Compton A. M., Bennett T. Regional haemodynamic effects of endothelin-1 and endothelin-3 in conscious Long Evans and Brattleboro rats. Br J Pharmacol. 1990 Jan;99(1):107–112. doi: 10.1111/j.1476-5381.1990.tb14662.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gardiner S. M., Compton A. M., Bennett T. Regional hemodynamic effects of endothelin-1 in conscious, unrestrained, Wistar rats. J Cardiovasc Pharmacol. 1989;13 (Suppl 5):S202–S204. doi: 10.1097/00005344-198900135-00057. [DOI] [PubMed] [Google Scholar]
  8. Gardiner S. M., Kemp P. A., Bennett T. Effects of NG-nitro-L-arginine methyl ester on vasodilator responses to acetylcholine, 5'-N-ethylcarboxamidoadenosine or salbutamol in conscious rats. Br J Pharmacol. 1991 Jul;103(3):1725–1732. doi: 10.1111/j.1476-5381.1991.tb09854.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hinojosa-Laborde C., Osborn J. W., Jr, Cowley A. W., Jr Hemodynamic effects of endothelin in conscious rats. Am J Physiol. 1989 Jun;256(6 Pt 2):H1742–H1746. doi: 10.1152/ajpheart.1989.256.6.H1742. [DOI] [PubMed] [Google Scholar]
  10. Ignarro L. J., Kadowitz P. J. The pharmacological and physiological role of cyclic GMP in vascular smooth muscle relaxation. Annu Rev Pharmacol Toxicol. 1985;25:171–191. doi: 10.1146/annurev.pa.25.040185.001131. [DOI] [PubMed] [Google Scholar]
  11. Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T. The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2863–2867. doi: 10.1073/pnas.86.8.2863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Karaki H., Nagase H. Low temperature augments the endothelium-dependent relaxation in isolated rat aorta. Eur J Pharmacol. 1987 Oct 6;142(1):129–132. doi: 10.1016/0014-2999(87)90661-3. [DOI] [PubMed] [Google Scholar]
  13. Kasuya Y., Ishikawa T., Yanagisawa M., Kimura S., Goto K., Masaki T. Mechanism of contraction to endothelin in isolated porcine coronary artery. Am J Physiol. 1989 Dec;257(6 Pt 2):H1828–H1835. doi: 10.1152/ajpheart.1989.257.6.H1828. [DOI] [PubMed] [Google Scholar]
  14. Marsden P. A., Danthuluri N. R., Brenner B. M., Ballermann B. J., Brock T. A. Endothelin action on vascular smooth muscle involves inositol trisphosphate and calcium mobilization. Biochem Biophys Res Commun. 1989 Jan 16;158(1):86–93. doi: 10.1016/s0006-291x(89)80180-9. [DOI] [PubMed] [Google Scholar]
  15. Moncada S. Eighth Gaddum Memorial Lecture. University of London Institute of Education, December 1980. Biological importance of prostacyclin. Br J Pharmacol. 1982 May;76(1):3–31. doi: 10.1111/j.1476-5381.1982.tb09186.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moncada S., Rees D. D., Schulz R., Palmer R. M. Development and mechanism of a specific supersensitivity to nitrovasodilators after inhibition of vascular nitric oxide synthesis in vivo. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2166–2170. doi: 10.1073/pnas.88.6.2166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moore P. K., al-Swayeh O. A., Chong N. W., Evans R. A., Gibson A. L-NG-nitro arginine (L-NOARG), a novel, L-arginine-reversible inhibitor of endothelium-dependent vasodilatation in vitro. Br J Pharmacol. 1990 Feb;99(2):408–412. doi: 10.1111/j.1476-5381.1990.tb14717.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rapoport R. M., Murad F. Endothelium-dependent and nitrovasodilator-induced relaxation of vascular smooth muscle: role of cyclic GMP. J Cyclic Nucleotide Protein Phosphor Res. 1983;9(4-5):281–296. [PubMed] [Google Scholar]
  19. Roberts M. F., Zygmunt A. C. Reflex and local thermal control of rabbit ear blood flow. Am J Physiol. 1984 Jun;246(6 Pt 2):R979–R984. doi: 10.1152/ajpregu.1984.246.6.R979. [DOI] [PubMed] [Google Scholar]
  20. Shirasaki Y., Su C. Endothelium removal augments vasodilation by sodium nitroprusside and sodium nitrite. Eur J Pharmacol. 1985 Aug 7;114(1):93–96. doi: 10.1016/0014-2999(85)90527-8. [DOI] [PubMed] [Google Scholar]
  21. White D. G., Lewis M. J., Griffith T. M., Edwards D. H., Henderson A. H. Influence of endothelium on drug-induced relaxation of the rabbit aorta. Eur J Pharmacol. 1986 Feb 11;121(1):19–23. doi: 10.1016/0014-2999(86)90387-0. [DOI] [PubMed] [Google Scholar]
  22. Yanagisawa M., Inoue A., Ishikawa T., Kasuya Y., Kimura S., Kumagaye S., Nakajima K., Watanabe T. X., Sakakibara S., Goto K. Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6964–6967. doi: 10.1073/pnas.85.18.6964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]
  24. Zamora M. R., O'Brien R. F., Rutherford R. B., Weil J. V. Serum endothelin-1 concentrations and cold provocation in primary Raynaud's phenomenon. Lancet. 1990 Nov 10;336(8724):1144–1147. doi: 10.1016/0140-6736(90)92766-b. [DOI] [PubMed] [Google Scholar]
  25. de Aguilera E. M., Irurzun A., Vila J. M., Aldasoro M., Galeote M. S., Lluch S. Role of endothelium and calcium channels in endothelin-induced contraction of human cerebral arteries. Br J Pharmacol. 1990 Mar;99(3):439–440. doi: 10.1111/j.1476-5381.1990.tb12945.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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