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. 1995 May;115(2):261–266. doi: 10.1111/j.1476-5381.1995.tb15872.x

Group B Streptococcus and E. coli LPS-induced NO-dependent hyporesponsiveness to noradrenaline in isolated intrapulmonary arteries of neonatal piglets.

E Villamor 1, F Pérez-Vizcaíno 1, T Ruiz 1, J C Leza 1, M Moro 1, J Tamargo 1
PMCID: PMC1908318  PMID: 7545518

Abstract

1. The effects of endotoxin (E. coli lipopolysaccharide, LPS) and heat inactivated group B Streptococcus (GBS) were studied on the contractile responses to noradrenaline (NA) in isolated pulmonary arteries and on the activity of the constitutive and inducible nitric oxide synthase (NOS) in lung fragments of neonatal piglets. 2. Short-term (< or = 5 h) incubation with LPS (1 micrograms ml-1) or GBS (3 x 10(7) colonies forming units ml-1) did not modify the vascular responsiveness to NA (10(-8) M-10(-4) M) in isolated intrapulmonary arteries. However, long-term incubation (20 h) with LPS or GBS produced a significant reduction in the maximal contractile responses and shifted the concentration-response curve for NA downwards. 3. Endothelium removal or the cyclo-oxygenase inhibitor meclofenamate (10(-5) M) did not affect the GBS- and LPS-induced hyporesponsiveness to NA. 4. The presence of the nitric oxide (NO) precursor, L-arginine (10(-5) M), 30 min prior to the contractility challenge increased the LPS- and GBS-induced pulmonary vascular hyporesponsiveness to NA. In contrast, the addition, prior to the challenge with NA, of the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) or coincubation with dexamethasone (3 x 10(-6) M), a potent inhibitor of the induction of NOS, or with the protein synthesis inhibitor cycloheximide (10(-5) M) completely restored the reactivity to NA in LPS- and GBS-treated pulmonary arteries.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Ablow R. C., Driscoll S. G., Effmann E. L., Gross I., Jolles C. J., Uauy R., Warshaw J. B. A comparison of early-onset group B steptococcal neonatal infection and the respiratory-distress syndrome of the newborn. N Engl J Med. 1976 Jan 8;294(2):65–70. doi: 10.1056/NEJM197601082940201. [DOI] [PubMed] [Google Scholar]
  2. Anthony B. F. Epidemiology of GBS in man. Antibiot Chemother (1971) 1985;35:10–16. doi: 10.1159/000410355. [DOI] [PubMed] [Google Scholar]
  3. Auguet M., Lonchampt M. O., Delaflotte S., Goulin-Schulz J., Chabrier P. E., Braquet P. Induction of nitric oxide synthase by lipoteichoic acid from Staphylococcus aureus in vascular smooth muscle cells. FEBS Lett. 1992 Feb 3;297(1-2):183–185. doi: 10.1016/0014-5793(92)80356-l. [DOI] [PubMed] [Google Scholar]
  4. Bowdy B. D., Marple S. L., Pauly T. H., Coonrod J. D., Gillespie M. N. Oxygen radical-dependent bacterial killing and pulmonary hypertension in piglets infected with group B streptococci. Am Rev Respir Dis. 1990 Mar;141(3):648–653. doi: 10.1164/ajrccm/141.3.648. [DOI] [PubMed] [Google Scholar]
  5. Brigham K. L., Meyrick B. Endotoxin and lung injury. Am Rev Respir Dis. 1986 May;133(5):913–927. [PubMed] [Google Scholar]
  6. Busse R., Mülsch A. Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells. FEBS Lett. 1990 Nov 26;275(1-2):87–90. doi: 10.1016/0014-5793(90)81445-t. [DOI] [PubMed] [Google Scholar]
  7. Cunha F. Q., Moss D. W., Leal L. M., Moncada S., Liew F. Y. Induction of macrophage parasiticidal activity by Staphylococcus aureus and exotoxins through the nitric oxide synthesis pathway. Immunology. 1993 Apr;78(4):563–567. [PMC free article] [PubMed] [Google Scholar]
  8. Dinh-Xuan A. T. Endothelial modulation of pulmonary vascular tone. Eur Respir J. 1992 Jun;5(6):757–762. [PubMed] [Google Scholar]
  9. Gibson R. L., Redding G. J., Truog W. E., Henderson W. R., Rubens C. E. Isogenic group B streptococci devoid of capsular polysaccharide or beta-hemolysin: pulmonary hemodynamic and gas exchange effects during bacteremia in piglets. Pediatr Res. 1989 Sep;26(3):241–245. doi: 10.1203/00006450-198909000-00017. [DOI] [PubMed] [Google Scholar]
  10. Groeneveld A. B., Nauta J. J., Thijs L. G. Peripheral vascular resistance in septic shock: its relation to outcome. Intensive Care Med. 1988;14(2):141–147. doi: 10.1007/BF00257468. [DOI] [PubMed] [Google Scholar]
  11. Hellerqvist C. G., Sundell H., Gettins P. Molecular basis for group B beta-hemolytic streptococcal disease. Proc Natl Acad Sci U S A. 1987 Jan;84(1):51–55. doi: 10.1073/pnas.84.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hemming V. G., O'Brien W. F., Fischer G. W., Golden S. M., Noble S. F. Studies of short-term pulmonary and peripheral vascular responses induced in oophorectomized sheep by the infusion of a group B streptococcal extract. Pediatr Res. 1984 Mar;18(3):266–269. doi: 10.1203/00006450-198403000-00010. [DOI] [PubMed] [Google Scholar]
  13. Ignarro L. J., Byrns R. E., Buga G. M., Wood K. S. Endothelium-derived relaxing factor from pulmonary artery and vein possesses pharmacologic and chemical properties identical to those of nitric oxide radical. Circ Res. 1987 Dec;61(6):866–879. doi: 10.1161/01.res.61.6.866. [DOI] [PubMed] [Google Scholar]
  14. Julou-Schaeffer G., Gray G. A., Fleming I., Schott C., Parratt J. R., Stoclet J. C. Loss of vascular responsiveness induced by endotoxin involves L-arginine pathway. Am J Physiol. 1990 Oct;259(4 Pt 2):H1038–H1043. doi: 10.1152/ajpheart.1990.259.4.H1038. [DOI] [PubMed] [Google Scholar]
  15. Knowles R. G., Merrett M., Salter M., Moncada S. Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem J. 1990 Sep 15;270(3):833–836. doi: 10.1042/bj2700833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marletta M. A., Yoon P. S., Iyengar R., Leaf C. D., Wishnok J. S. Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate. Biochemistry. 1988 Nov 29;27(24):8706–8711. doi: 10.1021/bi00424a003. [DOI] [PubMed] [Google Scholar]
  17. Maurer J. J., Mattingly S. J. Molecular analysis of lipoteichoic acid from Streptococcus agalactiae. J Bacteriol. 1991 Jan;173(2):487–494. doi: 10.1128/jb.173.2.487-494.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McKenna T. M. Prolonged exposure of rat aorta to low levels of endotoxin in vitro results in impaired contractility. Association with vascular cytokine release. J Clin Invest. 1990 Jul;86(1):160–168. doi: 10.1172/JCI114679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mitchell J. A., Kohlhaas K. L., Sorrentino R., Warner T. D., Murad F., Vane J. R. Induction by endotoxin of nitric oxide synthase in the rat mesentery: lack of effect on action of vasoconstrictors. Br J Pharmacol. 1993 May;109(1):265–270. doi: 10.1111/j.1476-5381.1993.tb13563.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moncada S., Palmer R. M., Higgs E. A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991 Jun;43(2):109–142. [PubMed] [Google Scholar]
  21. Nealon T. J., Mattingly S. J. Association of elevated levels of cellular lipoteichoic acids of group B streptococci with human neonatal disease. Infect Immun. 1983 Mar;39(3):1243–1251. doi: 10.1128/iai.39.3.1243-1251.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Palmer R. M., Ashton D. S., Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 1988 Jun 16;333(6174):664–666. doi: 10.1038/333664a0. [DOI] [PubMed] [Google Scholar]
  23. Perez-Vizcaino F., Casis O., Rodriguez R., Gomez L. A., Garcia Rafanell J., Tamargo J. Effects of the novel potassium channel opener, UR-8225, on contractile responses in rat isolated smooth muscle. Br J Pharmacol. 1993 Nov;110(3):1165–1171. doi: 10.1111/j.1476-5381.1993.tb13936.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Radomski M. W., Palmer R. M., Moncada S. Glucocorticoids inhibit the expression of an inducible, but not the constitutive, nitric oxide synthase in vascular endothelial cells. Proc Natl Acad Sci U S A. 1990 Dec;87(24):10043–10047. doi: 10.1073/pnas.87.24.10043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rojas J., Larsson L. E., Hellerqvist C. G., Brigham K. L., Gray M. E., Stahlman M. T. Pulmonary hemodynamic and ultrastructural changes associated with Group B streptococcal toxemia in adult sheep and newborn lambs. Pediatr Res. 1983 Dec;17(12):1002–1008. doi: 10.1203/00006450-198312000-00015. [DOI] [PubMed] [Google Scholar]
  26. Sakuma I., Stuehr D. J., Gross S. S., Nathan C., Levi R. Identification of arginine as a precursor of endothelium-derived relaxing factor. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8664–8667. doi: 10.1073/pnas.85.22.8664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Salter M., Knowles R. G., Moncada S. Widespread tissue distribution, species distribution and changes in activity of Ca(2+)-dependent and Ca(2+)-independent nitric oxide synthases. FEBS Lett. 1991 Oct 7;291(1):145–149. doi: 10.1016/0014-5793(91)81123-p. [DOI] [PubMed] [Google Scholar]
  28. Schreiber M. D., Covert R. F., Torgerson L. J. Hemodynamic effects of heat-killed group B beta-hemolytic streptococcus in newborn lambs: role of leukotriene D4. Pediatr Res. 1992 Feb;31(2):121–126. doi: 10.1203/00006450-199202000-00006. [DOI] [PubMed] [Google Scholar]
  29. Snapper J. R., Brigham K. L., Heflin A. C., Bomboy J. D., Jr, Graber S. E. Effects of endotoxemia on cyclic nucleotides in the unanesthetized sheep. J Lab Clin Med. 1983 Aug;102(2):240–249. [PubMed] [Google Scholar]
  30. Stamler J. S., Loh E., Roddy M. A., Currie K. E., Creager M. A. Nitric oxide regulates basal systemic and pulmonary vascular resistance in healthy humans. Circulation. 1994 May;89(5):2035–2040. doi: 10.1161/01.cir.89.5.2035. [DOI] [PubMed] [Google Scholar]
  31. Stuehr D. J., Griffith O. W. Mammalian nitric oxide synthases. Adv Enzymol Relat Areas Mol Biol. 1992;65:287–346. doi: 10.1002/9780470123119.ch8. [DOI] [PubMed] [Google Scholar]
  32. Szabó C., Mitchell J. A., Thiemermann C., Vane J. R. Nitric oxide-mediated hyporeactivity to noradrenaline precedes the induction of nitric oxide synthase in endotoxin shock. Br J Pharmacol. 1993 Mar;108(3):786–792. doi: 10.1111/j.1476-5381.1993.tb12879.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thiemermann C. The role of the L-arginine: nitric oxide pathway in circulatory shock. Adv Pharmacol. 1994;28:45–79. doi: 10.1016/s1054-3589(08)60493-7. [DOI] [PubMed] [Google Scholar]
  34. Warner A. E., Molina R. M., Brain J. D. Uptake of bloodborne bacteria by pulmonary intravascular macrophages and consequent inflammatory responses in sheep. Am Rev Respir Dis. 1987 Sep;136(3):683–690. doi: 10.1164/ajrccm/136.3.683. [DOI] [PubMed] [Google Scholar]
  35. Winkler G. C. Pulmonary intravascular macrophages in domestic animal species: review of structural and functional properties. Am J Anat. 1988 Mar;181(3):217–234. doi: 10.1002/aja.1001810302. [DOI] [PubMed] [Google Scholar]
  36. Zelenkov P., McLoughlin T., Johns R. A. Endotoxin enhances hypoxic constriction of rat aorta and pulmonary artery through induction of EDRF/NO synthase. Am J Physiol. 1993 Oct;265(4 Pt 1):L346–L354. doi: 10.1152/ajplung.1993.265.4.L346. [DOI] [PubMed] [Google Scholar]

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