Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Jun;103(2):1381–1384. doi: 10.1111/j.1476-5381.1991.tb09798.x

Inhibition by galanin and by high K+ of human basophil histamine release triggered by calcium ionophores but not responses induced by anti-IgE, chemotactic peptide or phorbol ester.

H Bergstrand 1, B Lundquist 1, B Ahrén 1
PMCID: PMC1908342  PMID: 1715794

Abstract

1. Galanin concentration-dependently blocked human leukocyte histamine release triggered by the calcium ionophores A23187 and ionomycin. Almost complete inhibition of release was recorded at 410 nM whereas 41 nM galanin mediated close to 50% inhibition of responses. 2. Pretreatment of the cells with pertussis toxin did not influence the inhibitory effects of galanin. 3. Leukocyte responses triggered by anti-IgE, the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) or 4 beta-phorbol 12-myristate 13-acetate (PMA) were not affected by galanin. 4. Ionophore-induced basophil histamine release, but not responses triggered by anti-IgE, FMLP or PMA, was inhibited when cells were challenged in medium containing high potassium (120mM). 5. We conclude that galanin and a depolarizing medium selectively inhibit ionophore-induced basophil histamine release.

Full text

PDF
1381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahrén B., Berggren P. O., Bokvist K., Rorsman P. Does galanin inhibit insulin secretion by opening of the ATP-regulated K+ channel in the beta-cell? Peptides. 1989 Mar-Apr;10(2):453–457. doi: 10.1016/0196-9781(89)90058-2. [DOI] [PubMed] [Google Scholar]
  2. Ahrén B., Böttcher G., Kowalyk S., Dunning B. E., Sundler F., Taborsky G. J., Jr Galanin is co-localized with noradrenaline and neuropeptide Y in dog pancreas and celiac ganglion. Cell Tissue Res. 1990 Jul;261(1):49–58. doi: 10.1007/BF00329437. [DOI] [PubMed] [Google Scholar]
  3. Ahrén B., Rorsman P., Berggren P. O. Galanin and the endocrine pancreas. FEBS Lett. 1988 Mar 14;229(2):233–237. doi: 10.1016/0014-5793(88)81131-1. [DOI] [PubMed] [Google Scholar]
  4. Amiranoff B., Lorinet A. M., Laburthe M. Galanin receptor in the rat pancreatic beta cell line Rin m 5F. Molecular characterization by chemical cross-linking. J Biol Chem. 1989 Dec 5;264(34):20714–20717. [PubMed] [Google Scholar]
  5. Bergstrand H., Lundquist B., Michelsen P. Induction of human basophil histamine release by a novel protein kinase C activator, sn-1,2-isopropylidene-3-decanoyl-glycerol (IpOCOC9): partial characterization of secretagogue characteristics. Eur J Haematol. 1988 Nov;41(5):467–477. doi: 10.1111/j.1600-0609.1988.tb00229.x. [DOI] [PubMed] [Google Scholar]
  6. Bindslev Jensen C., Clementsen P., Stahl Skov P., Svendsen U. G., Thastrup O., Norn S. Comparison of the action of calcium antagonists on basophil histamine release. Allergy. 1987 Aug;42(6):441–446. doi: 10.1111/j.1398-9995.1987.tb00360.x. [DOI] [PubMed] [Google Scholar]
  7. Conroy M. C., Kennedy J. A., Thueson D. O. Differential inhibition of histamine release from human basophils induced by antigen, anti-IgE or N-formyl-L-methionyl-L-leucyl-L-phenylalanine. Int Arch Allergy Appl Immunol. 1985;77(1-2):222–224. doi: 10.1159/000233793. [DOI] [PubMed] [Google Scholar]
  8. Dunne M. J., Bullett M. J., Li G. D., Wollheim C. B., Petersen O. H. Galanin activates nucleotide-dependent K+ channels in insulin-secreting cells via a pertussis toxin-sensitive G-protein. EMBO J. 1989 Feb;8(2):413–420. doi: 10.1002/j.1460-2075.1989.tb03392.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dunning B. E., Taborsky G. J., Jr Galanin--sympathetic neurotransmitter in endocrine pancreas? Diabetes. 1988 Sep;37(9):1157–1162. doi: 10.2337/diab.37.9.1157. [DOI] [PubMed] [Google Scholar]
  10. Mohr F. C., Fewtrell C. Depolarization of rat basophilic leukemia cells inhibits calcium uptake and exocytosis. J Cell Biol. 1987 Mar;104(3):783–792. doi: 10.1083/jcb.104.3.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nilsson T., Arkhammar P., Rorsman P., Berggren P. O. Suppression of insulin release by galanin and somatostatin is mediated by a G-protein. An effect involving repolarization and reduction in cytoplasmic free Ca2+ concentration. J Biol Chem. 1989 Jan 15;264(2):973–980. [PubMed] [Google Scholar]
  12. Sharp G. W., Le Marchand-Brustel Y., Yada T., Russo L. L., Bliss C. R., Cormont M., Monge L., Van Obberghen E. Galanin can inhibit insulin release by a mechanism other than membrane hyperpolarization or inhibition of adenylate cyclase. J Biol Chem. 1989 May 5;264(13):7302–7309. [PubMed] [Google Scholar]
  13. Ullrich S., Wollheim C. B. Galanin inhibits insulin secretion by direct interference with exocytosis. FEBS Lett. 1989 Apr 24;247(2):401–404. doi: 10.1016/0014-5793(89)81379-1. [DOI] [PubMed] [Google Scholar]
  14. Warner J. A., MacGlashan D. W., Jr Protein kinase C (PKC) changes in human basophils. IgE-mediated activation is accompanied by an increase in total PKC activity. J Immunol. 1989 Mar 1;142(5):1669–1677. [PubMed] [Google Scholar]
  15. Warner J. A., Yancey K. B., MacGlashan D. W., Jr The effect of pertussis toxin on mediator release from human basophils. J Immunol. 1987 Jul 1;139(1):161–165. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES