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. 1991 Jun;103(2):1470–1474. doi: 10.1111/j.1476-5381.1991.tb09813.x

Epithelium-dependent potentiation of anaphylactic contractions by beta-endorphin in tracheae isolated from actively sensitized guinea-pigs.

A J Van Oosterhout 1, L Celeda 1, K C Delsman 1, D de Wied 1, F P Nijkamp 1
PMCID: PMC1908357  PMID: 1884103

Abstract

1. It has been shown that opioid peptides modulate airway function. In the present study, the effect of beta-endorphin on antigen-induced contractions of isolated tracheal rings from actively sensitized guinea-pigs has been studied. 2. beta-Endorphin had a concentration-dependent bimodal effect on anaphylactic contractions of the trachea. Low concentrations of beta-endorphin (10(-10) and 10(-8) M) significantly potentiated anaphylactic contractions, whereas higher concentrations (10(-7) and 10(-6) M) significantly suppressed anaphylactic contractions of guinea-pig trachea. 3. beta-Endorphin in concentrations of 10(-8) M and 10(-7) M did not affect the responsiveness of the tracheal rings to histamine or leukotriene D4. This indicates that beta-endorphin does not influence the responsiveness of tracheal smooth muscle to anaphylactic mediators. 4. In the presence of the non-selective opioid receptor antagonist naloxone, 10(-8) M beta-endorphin still potentiated the anaphylactic contractions of the trachea. In addition, an equimolar concentration of des-Tyr1-beta-endorphin, a fragment of beta-endorphin without opioid-like activity, also potentiated anaphylactic contractions. The potentiation of anaphylactic contraction by 10(-8) M beta-endorphin is not therefore mediated by classical opioid-receptors. 5. In the presence of naloxone, 10(-7) M, beta-endorphin did not suppress anaphylactic contractions of the trachea. Thus, the suppression of anaphylactic contraction is mediated via a classical opioid-receptor. 6. In epithelium-denuded trachea, both 10(-8) and 10(-7) M beta-endorphin suppressed the anaphylactic contractions, whereas 10(-8) and 10(-7) M des-Tyr1-beta-endorphin did not affect anaphylactic contractions.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Amir S. Opiate antagonists improve survival in anaphylactic shock. Eur J Pharmacol. 1982 May 7;80(1):161–162. doi: 10.1016/0014-2999(82)90194-7. [DOI] [PubMed] [Google Scholar]
  2. Badawy A. A., Evans M., Punjani N. F., Morgan C. J. Does naloxone always act as an opiate antagonist? Life Sci. 1983;33 (Suppl 1):739–742. doi: 10.1016/0024-3205(83)90608-2. [DOI] [PubMed] [Google Scholar]
  3. Barnes P. J., Belvisi M. G., Rogers D. F. Modulation of neurogenic inflammation: novel approaches to inflammatory disease. Trends Pharmacol Sci. 1990 May;11(5):185–189. doi: 10.1016/0165-6147(90)90112-l. [DOI] [PubMed] [Google Scholar]
  4. Bienenstock J. An update on mast cell heterogeneity. J Allergy Clin Immunol. 1988 May;81(5 Pt 1):763–769. doi: 10.1016/0091-6749(88)90929-3. [DOI] [PubMed] [Google Scholar]
  5. De Wied D., Jolles J. Neuropeptides derived from pro-opiocortin: behavioral, physiological, and neurochemical effects. Physiol Rev. 1982 Jul;62(3):976–1059. doi: 10.1152/physrev.1982.62.3.976. [DOI] [PubMed] [Google Scholar]
  6. Dusser D. J., Jacoby D. B., Djokic T. D., Rubinstein I., Borson D. B., Nadel J. A. Virus induces airway hyperresponsiveness to tachykinins: role of neutral endopeptidase. J Appl Physiol (1985) 1989 Oct;67(4):1504–1511. doi: 10.1152/jappl.1989.67.4.1504. [DOI] [PubMed] [Google Scholar]
  7. Erdös E. G., Skidgel R. A. Neutral endopeptidase 24.11 (enkephalinase) and related regulators of peptide hormones. FASEB J. 1989 Feb;3(2):145–151. [PubMed] [Google Scholar]
  8. Fischer E. G. Opioid peptides modulate immune functions. A review. Immunopharmacol Immunotoxicol. 1988;10(3):265–326. doi: 10.3109/08923978809041423. [DOI] [PubMed] [Google Scholar]
  9. Karlsson J. A., Lanner A. S., Persson C. G. Airway opioid receptors mediate inhibition of cough and reflex bronchoconstriction in guinea pigs. J Pharmacol Exp Ther. 1990 Feb;252(2):863–868. [PubMed] [Google Scholar]
  10. Kitamura Y. Heterogeneity of mast cells and phenotypic change between subpopulations. Annu Rev Immunol. 1989;7:59–76. doi: 10.1146/annurev.iy.07.040189.000423. [DOI] [PubMed] [Google Scholar]
  11. Lolait S. J., Clements J. A., Markwick A. J., Cheng C., McNally M., Smith A. I., Funder J. W. Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages. J Clin Invest. 1986 Jun;77(6):1776–1779. doi: 10.1172/JCI112501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martin J., Prystowsky M. B., Angeletti R. H. Preproenkephalin mRNA in T-cells, macrophages, and mast cells. J Neurosci Res. 1987;18(1):82–87. doi: 10.1002/jnr.490180114. [DOI] [PubMed] [Google Scholar]
  13. Mediratta P. K., Das N., Gupta V. S., Sen P. Modulation of humoral immune responses by endogenous opioids. J Allergy Clin Immunol. 1988 Jan;81(1):27–32. doi: 10.1016/0091-6749(88)90216-3. [DOI] [PubMed] [Google Scholar]
  14. Miadonna A., Leggieri E., Tedeschi A., Lorini M., Froldi M., Zanussi C. Study of the effect of some neuropeptides and endogenous opioid peptides on in vitro histamine release from human lung mast cells and peripheral blood basophils. Agents Actions. 1988 Aug;25(1-2):11–16. doi: 10.1007/BF01969087. [DOI] [PubMed] [Google Scholar]
  15. Olson G. A., Olson R. D., Kastin A. J. Endogenous opiates: 1987. Peptides. 1989 Jan-Feb;10(1):205–236. doi: 10.1016/0196-9781(89)90098-3. [DOI] [PubMed] [Google Scholar]
  16. Popa V., Rients P. The effect of inhaled naloxone on resting bronchial tone and exercise-induced asthma. Am Rev Respir Dis. 1989 Mar;139(3):702–709. doi: 10.1164/ajrccm/139.3.702. [DOI] [PubMed] [Google Scholar]
  17. Rogers D. F., Barnes P. J. Opioid inhibition of neurally mediated mucus secretion in human bronchi. Lancet. 1989 Apr 29;1(8644):930–932. doi: 10.1016/s0140-6736(89)92509-9. [DOI] [PubMed] [Google Scholar]
  18. Sawynok J., Pinsky C., LaBella F. S. On the specificity of naloxone as an opiate antagonist. Life Sci. 1979 Nov 5;25(19):1621–1632. doi: 10.1016/0024-3205(79)90403-x. [DOI] [PubMed] [Google Scholar]
  19. Shanahan F., Lee T. D., Bienenstock J., Befus A. D. The influence of endorphins on peritoneal and mucosal mast cell secretion. J Allergy Clin Immunol. 1984 Oct;74(4 Pt 1):499–504. doi: 10.1016/0091-6749(84)90385-3. [DOI] [PubMed] [Google Scholar]
  20. Sibinga N. E., Goldstein A. Opioid peptides and opioid receptors in cells of the immune system. Annu Rev Immunol. 1988;6:219–249. doi: 10.1146/annurev.iy.06.040188.001251. [DOI] [PubMed] [Google Scholar]
  21. Sydbom A. Characteristics of beta-endorphin-induced histamine release from rat serosal mast cells. Comparison with neurotensin, dynorphin and compound 48/80. Naunyn Schmiedebergs Arch Pharmacol. 1988 Nov;338(5):567–572. doi: 10.1007/BF00179331. [DOI] [PubMed] [Google Scholar]
  22. Van Oosterhout A. J., Nijkamp F. P. Anterior hypothalamic lesions decrease anaphylactic contractions in guinea pig trachea in vitro by reducing histamine and LTC4 reactivity. Int J Immunopharmacol. 1986;8(8):975–983. doi: 10.1016/0192-0561(86)90100-1. [DOI] [PubMed] [Google Scholar]

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