Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1992 Mar;105(3):686–690. doi: 10.1111/j.1476-5381.1992.tb09039.x

Pharmacological characterization of angiotensin-induced depolarizations of rat superior cervical ganglion in vitro.

A B Hawcock 1, J C Barnes 1, A D Michel 1
PMCID: PMC1908448  PMID: 1628155

Abstract

1. The depolarizing responses to angiotensin II and angiotensin III of the rat superior cervical ganglion have been characterized in vitro, by the use of peptidase inhibitors, peptide and non-peptide antagonists and dithiothreitol (DTT). 2. Angiotensin II and III depolarized the ganglion in a concentration-related manner. Angiotensin II was approximately 30 fold more potent than angiotensin III. 3. The endopeptidase inhibitor, bacitracin, increased the potency of angiotensin II and III by approximately 4 and 20 fold respectively. The aminopeptidase inhibitor, amastatin, further increased the potency of angiotensin III (but not angiotensin II) by approximately 4 fold. In the presence of bacitracin and amastatin, angiotensin II and III were equipotent. 4. The peptide antagonist [Ile7]angiotensin III (0.01-0.3 microM) produced a non-parallel rightward displacement of the angiotensin II concentration-response curve, with a suppression of the maximum response. The potency of [Ile7]angiotensin III was increased by bacitracin and amastatin. 5. The AT1-selective non-peptide antagonist losartan (DuP 753; 0.03 and 0.1 microM) produced a parallel rightward displacement of the angiotensin II concentration-response curve, with an apparent pKB of 8.3 +/- 0.1. A higher concentration of losartan (0.3 microM) depressed the maximum agonist response by 32 +/- 6.5%, possibly reflecting non-competitive behaviour of the antagonist. The potency of losartan was not influenced by bacitracin. 6. The AT2-selective non-peptide antagonist, PD123177 (3 microM) failed to antagonize the angiotensin II-induced depolarizations. 7. DTT (1 mM) produced a 22% reduction of the maximum response to angiotensin II.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
686

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown D. A., Constanti A., Marsh S. Angiotensin mimics the action of muscarinic agonists on rat sympathetic neurones. Brain Res. 1980 Jul 14;193(2):614–619. doi: 10.1016/0006-8993(80)90200-0. [DOI] [PubMed] [Google Scholar]
  2. Bumpus F. M., Catt K. J., Chiu A. T., DeGasparo M., Goodfriend T., Husain A., Peach M. J., Taylor D. G., Jr, Timmermans P. B. Nomenclature for angiotensin receptors. A report of the Nomenclature Committee of the Council for High Blood Pressure Research. Hypertension. 1991 May;17(5):720–721. doi: 10.1161/01.hyp.17.5.720. [DOI] [PubMed] [Google Scholar]
  3. Carrithers M. D., Raman V. K., Masuda S., Weyhenmeyer J. A. Effect of angiotensin II and III on inositol polyphosphate production in differentiated NG108-15 hybrid cells. Biochem Biophys Res Commun. 1990 Mar 30;167(3):1200–1205. doi: 10.1016/0006-291x(90)90651-3. [DOI] [PubMed] [Google Scholar]
  4. Chiu A. T., Herblin W. F., McCall D. E., Ardecky R. J., Carini D. J., Duncia J. V., Pease L. J., Wong P. C., Wexler R. R., Johnson A. L. Identification of angiotensin II receptor subtypes. Biochem Biophys Res Commun. 1989 Nov 30;165(1):196–203. doi: 10.1016/0006-291x(89)91054-1. [DOI] [PubMed] [Google Scholar]
  5. Chiu A. T., McCall D. E., Nguyen T. T., Carini D. J., Duncia J. V., Herblin W. F., Uyeda R. T., Wong P. C., Wexler R. R., Johnson A. L. Discrimination of angiotensin II receptor subtypes by dithiothreitol. Eur J Pharmacol. 1989 Oct 24;170(1-2):117–118. doi: 10.1016/0014-2999(89)90145-3. [DOI] [PubMed] [Google Scholar]
  6. Criscione L., Thomann H., Whitebread S., de Gasparo M., Bühlmayer P., Herold P., Ostermayer F., Kamber B. Binding characteristics and vascular effects of various angiotensin II antagonists. J Cardiovasc Pharmacol. 1990;16 (Suppl 4):S56–S59. doi: 10.1097/00005344-199016004-00012. [DOI] [PubMed] [Google Scholar]
  7. Dun N. J., Nishi S., Karczmar A. G. An analysis of the effect of angiotensin II on mammalian ganglion cells. J Pharmacol Exp Ther. 1978 Mar;204(3):669–675. [PubMed] [Google Scholar]
  8. Fleisch J. H., Krzan M. C., Titus E. Pharmacologic receptor activity of rabbit aorta. Effect of dithiothreitol and N-ethylmaleimide. Circ Res. 1973 Sep;33(3):284–290. doi: 10.1161/01.res.33.3.284. [DOI] [PubMed] [Google Scholar]
  9. Gunther S. Characterization of angiotensin II receptor subtypes in rat liver. J Biol Chem. 1984 Jun 25;259(12):7622–7629. [PubMed] [Google Scholar]
  10. Ireland S. J. Origin of 5-hydroxytryptamine-induced hyperpolarization of the rat superior cervical ganglion and vagus nerve. Br J Pharmacol. 1987 Oct;92(2):407–416. doi: 10.1111/j.1476-5381.1987.tb11337.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jackson T. R., Blair L. A., Marshall J., Goedert M., Hanley M. R. The mas oncogene encodes an angiotensin receptor. Nature. 1988 Sep 29;335(6189):437–440. doi: 10.1038/335437a0. [DOI] [PubMed] [Google Scholar]
  12. Kugler P. Aminopeptidase A is angiotensinase A. II. Biochemical studies on aminopeptidase A and M in rat kidney homogenate. Histochemistry. 1982;74(2):247–261. doi: 10.1007/BF00495834. [DOI] [PubMed] [Google Scholar]
  13. Moore A. F., HAll M. M., Khairallah P. A. A comparison of the effects of angiotensin II and heptapeptide on smooth muscle (vascular and uterine). Eur J Pharmacol. 1976 Sep;39(1):101–107. doi: 10.1016/0014-2999(76)90117-5. [DOI] [PubMed] [Google Scholar]
  14. Peach M. J. Renin-angiotensin system: biochemistry and mechanisms of action. Physiol Rev. 1977 Apr;57(2):313–370. doi: 10.1152/physrev.1977.57.2.313. [DOI] [PubMed] [Google Scholar]
  15. Reit E. Actions of angiotensin on the adrenal medulla and autonomic ganglia. Fed Proc. 1972 Jul-Aug;31(4):1338–1343. [PubMed] [Google Scholar]
  16. Scanlon M. N., Moore G. J. Classification of angiotensin receptors in rat isolated uterus, portal vein, and aorta with the novel competitive antagonist sarmesin. Pharmacology. 1988;37(3):137–147. doi: 10.1159/000138457. [DOI] [PubMed] [Google Scholar]
  17. Speth R. C., Kim K. H. Discrimination of two angiotensin II receptor subtypes with a selective agonist analogue of angiotensin II, p-aminophenylalanine6 angiotensin II. Biochem Biophys Res Commun. 1990 Jun 29;169(3):997–1006. doi: 10.1016/0006-291x(90)91993-3. [DOI] [PubMed] [Google Scholar]
  18. Spielman W. S., Davis J. O., Freeman R. H. Des-Asp-1-angiotensin II. possible role in mediating the renin--angiotensin response in the rat. Proc Soc Exp Biol Med. 1976 Jan;151(1):177–182. doi: 10.3181/00379727-151-39169. [DOI] [PubMed] [Google Scholar]
  19. Whitebread S., Mele M., Kamber B., de Gasparo M. Preliminary biochemical characterization of two angiotensin II receptor subtypes. Biochem Biophys Res Commun. 1989 Aug 30;163(1):284–291. doi: 10.1016/0006-291x(89)92133-5. [DOI] [PubMed] [Google Scholar]
  20. Wong P. C., Price W. A., Jr, Chiu A. T., Carini D. J., Duncia J. V., Johnson A. L., Wexler R. R., Timmermans P. B. Nonpeptide angiotensin II receptor antagonists. Studies with EXP9270 and DuP 753. Hypertension. 1990 Jun;15(6 Pt 2):823–834. doi: 10.1161/01.hyp.15.6.823. [DOI] [PubMed] [Google Scholar]
  21. de Gasparo M., Whitebread S., Mele M., Motani A. S., Whitcombe P. J., Ramjoué H. P., Kamber B. Biochemical characterization of two angiotensin II receptor subtypes in the rat. J Cardiovasc Pharmacol. 1990;16 (Suppl 4):S31–S35. doi: 10.1097/00005344-199016004-00008. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES