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. 1991 Oct;104(2):391–398. doi: 10.1111/j.1476-5381.1991.tb12441.x

In vitro modulation of the eosinophil-dependent enhancement of the permeability of the bronchial mucosa.

C A Herbert 1, D Edwards 1, J R Boot 1, C Robinson 1
PMCID: PMC1908556  PMID: 1797305

Abstract

1. Basolateral to apical albumin flux has been measured in sheets of bovine bronchial and tracheal mucosa mounted in vitro. 2. Addition of guinea-pig peritoneal eosinophils or neutrophils to the basolateral side of such tissues had no significant influence on the transmucosal flux of albumin in either the bronchial or tracheal mucosa. 3. Stimulation of eosinophils or neutrophils by the calcium ionophore A23187, or by their presentation to an opsonized airways mucosa, resulted in a significant increase in the transbronchial flux of albumin. This effect was seen after only 60 min incubation of the leucocytes with the bronchial mucosa, and was no greater when the contact time was extended to 180 min. Incubation of bronchial mucosal tissues with 1 mg ml-1 polyarginine for 3 h produced a significant increase in albumin flux, but was ineffective at 0.5 mg ml-1. 4. In contrast to the bronchial mucosa, the tracheal mucosa appeared resistant to the effects of stimulated eosinophils and neutrophils. 5. The lipoxygenase inhibitor AA-861 failed to influence the ability of eosinophils to augment the transmembrane flux of albumin. However, insertion of a Millipore filter mask between the eosinophils and the bronchial mucosa significantly inhibited the eosinophil-dependent enhancement of mucosal permeability. 6. The broad spectrum antiproteinase alpha 2-macroglobulin achieved almost total ablation of the action of stimulated eosinophils in the bronchial mucosa. These results suggest that proteinases may make a significant contribution to the genesis of epithelial injury, whereas leukotrienes do not.

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Selected References

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  1. Agosti J. M., Altman L. C., Ayars G. H., Loegering D. A., Gleich G. J., Klebanoff S. J. The injurious effect of eosinophil peroxidase, hydrogen peroxide, and halides on pneumocytes in vitro. J Allergy Clin Immunol. 1987 Mar;79(3):496–504. doi: 10.1016/0091-6749(87)90368-x. [DOI] [PubMed] [Google Scholar]
  2. Ashida Y., Saijo T., Kuriki H., Makino H., Terao S., Maki Y. Pharmacological profile of AA-861, a 5-lipoxygenase inhibitor. Prostaglandins. 1983 Dec;26(6):955–972. doi: 10.1016/0090-6980(83)90157-0. [DOI] [PubMed] [Google Scholar]
  3. Ayars G. H., Altman L. C., McManus M. M., Agosti J. M., Baker C., Luchtel D. L., Loegering D. A., Gleich G. J. Injurious effect of the eosinophil peroxide-hydrogen peroxide-halide system and major basic protein on human nasal epithelium in vitro. Am Rev Respir Dis. 1989 Jul;140(1):125–131. doi: 10.1164/ajrccm/140.1.125. [DOI] [PubMed] [Google Scholar]
  4. Butterworth A. E., Wassom D. L., Gleich G. J., Loegering D. A., David J. R. Damage to schistosomula of Schistosoma mansoni induced directly by eosinophil major basic protein. J Immunol. 1979 Jan;122(1):221–229. [PubMed] [Google Scholar]
  5. Campbell A. M., Harper S. T., Hallam C., Wells E., Mann J., Robinson C. Enhancement of leukotriene C4 release from primate airway macrophages by cellular interactions. Br J Pharmacol. 1989 Oct;98(2):637–645. doi: 10.1111/j.1476-5381.1989.tb12638.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis W. B., Fells G. A., Sun X. H., Gadek J. E., Venet A., Crystal R. G. Eosinophil-mediated injury to lung parenchymal cells and interstitial matrix. A possible role for eosinophils in chronic inflammatory disorders of the lower respiratory tract. J Clin Invest. 1984 Jul;74(1):269–278. doi: 10.1172/JCI111411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Diaz P., Gonzalez M. C., Galleguillos F. R., Ancic P., Cromwell O., Shepherd D., Durham S. R., Gleich G. J., Kay A. B. Leukocytes and mediators in bronchoalveolar lavage during allergen-induced late-phase asthmatic reactions. Am Rev Respir Dis. 1989 Jun;139(6):1383–1389. doi: 10.1164/ajrccm/139.6.1383. [DOI] [PubMed] [Google Scholar]
  8. Djukanović R., Roche W. R., Wilson J. W., Beasley C. R., Twentyman O. P., Howarth R. H., Holgate S. T. Mucosal inflammation in asthma. Am Rev Respir Dis. 1990 Aug;142(2):434–457. doi: 10.1164/ajrccm/142.2.434. [DOI] [PubMed] [Google Scholar]
  9. Dunnill M. S., Massarella G. R., Anderson J. A. A comparison of the quantitative anatomy of the bronchi in normal subjects, in status asthmaticus, in chronic bronchitis, and in emphysema. Thorax. 1969 Mar;24(2):176–179. doi: 10.1136/thx.24.2.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elia C., Bucca C., Rolla G., Scappaticci E., Cantino D. A freeze-fracture study of human bronchial epithelium in normal, bronchitic and asthmatic subjects. J Submicrosc Cytol Pathol. 1988 Jul;20(3):509–517. [PubMed] [Google Scholar]
  11. Fabbri L. M., Boschetto P., Zocca E., Milani G., Pivirotto F., Plebani M., Burlina A., Licata B., Mapp C. E. Bronchoalveolar neutrophilia during late asthmatic reactions induced by toluene diisocyanate. Am Rev Respir Dis. 1987 Jul;136(1):36–42. doi: 10.1164/ajrccm/136.1.36. [DOI] [PubMed] [Google Scholar]
  12. Filley W. V., Holley K. E., Kephart G. M., Gleich G. J. Identification by immunofluorescence of eosinophil granule major basic protein in lung tissues of patients with bronchial asthma. Lancet. 1982 Jul 3;2(8288):11–16. doi: 10.1016/s0140-6736(82)91152-7. [DOI] [PubMed] [Google Scholar]
  13. Flavahan N. A., Slifman N. R., Gleich G. J., Vanhoutte P. M. Human eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epithelium. Am Rev Respir Dis. 1988 Sep;138(3):685–688. doi: 10.1164/ajrccm/138.3.685. [DOI] [PubMed] [Google Scholar]
  14. Frigas E., Loegering D. A., Gleich G. J. Cytotoxic effects of the guinea pig eosinophil major basic protein on tracheal epithelium. Lab Invest. 1980 Jan;42(1):35–43. [PubMed] [Google Scholar]
  15. Gleich G. J., Adolphson C. R. The eosinophilic leukocyte: structure and function. Adv Immunol. 1986;39:177–253. doi: 10.1016/s0065-2776(08)60351-x. [DOI] [PubMed] [Google Scholar]
  16. Gleich G. J., Flavahan N. A., Fujisawa T., Vanhoutte P. M. The eosinophil as a mediator of damage to respiratory epithelium: a model for bronchial hyperreactivity. J Allergy Clin Immunol. 1988 May;81(5 Pt 1):776–781. doi: 10.1016/0091-6749(88)90931-1. [DOI] [PubMed] [Google Scholar]
  17. Hastie A. T., Loegering D. A., Gleich G. J., Kueppers F. The effect of purified human eosinophil major basic protein on mammalian ciliary activity. Am Rev Respir Dis. 1987 Apr;135(4):848–853. doi: 10.1164/arrd.1987.135.4.848. [DOI] [PubMed] [Google Scholar]
  18. Ilowite J. S., Bennett W. D., Sheetz M. S., Groth M. L., Nierman D. M. Permeability of the bronchial mucosa to 99mTc-DTPA in asthma. Am Rev Respir Dis. 1989 May;139(5):1139–1143. doi: 10.1164/ajrccm/139.5.1139. [DOI] [PubMed] [Google Scholar]
  19. Jeffery P. K., Wardlaw A. J., Nelson F. C., Collins J. V., Kay A. B. Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am Rev Respir Dis. 1989 Dec;140(6):1745–1753. doi: 10.1164/ajrccm/140.6.1745. [DOI] [PubMed] [Google Scholar]
  20. Laitinen L. A., Heino M., Laitinen A., Kava T., Haahtela T. Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis. 1985 Apr;131(4):599–606. doi: 10.1164/arrd.1985.131.4.599. [DOI] [PubMed] [Google Scholar]
  21. Lam S., Leriche J. C., Kijek K., Phillips D. Effect of bronchial lavage volume on cellular and protein recovery. Chest. 1985 Dec;88(6):856–859. doi: 10.1378/chest.88.6.856. [DOI] [PubMed] [Google Scholar]
  22. Motojima S., Frigas E., Loegering D. A., Gleich G. J. Toxicity of eosinophil cationic proteins for guinea pig tracheal epithelium in vitro. Am Rev Respir Dis. 1989 Mar;139(3):801–805. doi: 10.1164/ajrccm/139.3.801. [DOI] [PubMed] [Google Scholar]
  23. NAYLOR B. The shedding of the mucosa of the bronchial tree in asthma. Thorax. 1962 Mar;17:69–72. doi: 10.1136/thx.17.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Northover B. J. Continuous fluorimetric assessment of the changes in cytoplasmic calcium concentration during exposure of rat isolated myocardium to conditions of simulated ischaemia. Br J Pharmacol. 1990 Jul;100(3):477–482. doi: 10.1111/j.1476-5381.1990.tb15832.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Persson C. G., Erjefält I., Gustafsson B., Luts A. Subepithelial hydrostatic pressure may regulate plasma exudation across the mucosa. Int Arch Allergy Appl Immunol. 1990;92(2):148–153. doi: 10.1159/000235206. [DOI] [PubMed] [Google Scholar]
  26. Persson C. G., Erjefält I. Inflammatory leakage of macromolecules from the vascular compartment into the tracheal lumen. Acta Physiol Scand. 1986 Apr;126(4):615–616. doi: 10.1111/j.1748-1716.1986.tb07863.x. [DOI] [PubMed] [Google Scholar]
  27. Persson C. G. Plasma exudation and asthma. Lung. 1988;166(1):1–23. doi: 10.1007/BF02714025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Persson C. G. Role of plasma exudation in asthmatic airways. Lancet. 1986 Nov 15;2(8516):1126–1129. doi: 10.1016/s0140-6736(86)90533-7. [DOI] [PubMed] [Google Scholar]
  29. Pincus S. H. Production of eosinophil-rich guinea pig peritoneal exudates. Blood. 1978 Jul;52(1):127–134. [PubMed] [Google Scholar]
  30. Ranga V., Powers M. A., Padilla M., Strope G. L., Fowler L., Kleinerman J. Effect of allergic bronchoconstriction on airways epithelial permeability to large polar solutes in the guinea pig. Am Rev Respir Dis. 1983 Dec;128(6):1065–1070. doi: 10.1164/arrd.1983.128.6.1065. [DOI] [PubMed] [Google Scholar]
  31. Reddy V. Y., Pizzo S. V., Weiss S. J. Functional inactivation and structural disruption of human alpha 2-macroglobulin by neutrophils and eosinophils. J Biol Chem. 1989 Aug 15;264(23):13801–13809. [PubMed] [Google Scholar]
  32. Robinson C., Holgate S. T. New perspectives on the putative role of eicosanoids in airway hyperresponsiveness. J Allergy Clin Immunol. 1985 Aug;76(2 Pt 1):140–144. doi: 10.1016/0091-6749(85)90691-8. [DOI] [PubMed] [Google Scholar]
  33. Ryley H. C., Brogan T. D. Variation in the composition of sputum in chronic chest diseases. Br J Exp Pathol. 1968 Dec;49(6):625–633. [PMC free article] [PubMed] [Google Scholar]
  34. Sanjar S., Aoki S., Boubekeur K., Burrows L., Colditz I., Chapman I., Morley J. Inhibition of PAF-induced eosinophil accumulation in pulmonary airways of guinea pigs by anti-asthma drugs. Jpn J Pharmacol. 1989 Oct;51(2):167–172. doi: 10.1254/jjp.51.167. [DOI] [PubMed] [Google Scholar]
  35. Sanjar S., Aoki S., Boubekeur K., Chapman I. D., Smith D., Kings M. A., Morley J. Eosinophil accumulation in pulmonary airways of guinea-pigs induced by exposure to an aerosol of platelet-activating factor: effect of anti-asthma drugs. Br J Pharmacol. 1990 Feb;99(2):267–272. doi: 10.1111/j.1476-5381.1990.tb14692.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Sanjar S., Aoki S., Kristersson A., Smith D., Morley J. Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea-pigs: the effect of anti-asthma drugs. Br J Pharmacol. 1990 Apr;99(4):679–686. doi: 10.1111/j.1476-5381.1990.tb12989.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sun F. F., Czuk C. I., Taylor B. M. Arachidonic acid metabolism in guinea pig eosinophils: synthesis of thromboxane B2 and leukotriene B4 in response to soluble or particulate activators. J Leukoc Biol. 1989 Aug;46(2):152–160. doi: 10.1002/jlb.46.2.152. [DOI] [PubMed] [Google Scholar]
  38. Vanhoutte P. M. Epithelium derived relaxing factor: myth or reality? Thorax. 1988 Sep;43(9):665–668. doi: 10.1136/thx.43.9.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wardlaw A. J., Dunnette S., Gleich G. J., Collins J. V., Kay A. B. Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity. Am Rev Respir Dis. 1988 Jan;137(1):62–69. doi: 10.1164/ajrccm/137.1.62. [DOI] [PubMed] [Google Scholar]
  40. Webber S. E., Widdicombe J. G. The transport of albumin across the ferret in vitro whole trachea. J Physiol. 1989 Jan;408:457–472. doi: 10.1113/jphysiol.1989.sp017470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Yukawa T., Read R. C., Kroegel C., Rutman A., Chung K. F., Wilson R., Cole P. J., Barnes P. J. The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro. Am J Respir Cell Mol Biol. 1990 Apr;2(4):341–353. doi: 10.1165/ajrcmb/2.4.341. [DOI] [PubMed] [Google Scholar]

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