Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Oct;104(2):349–354. doi: 10.1111/j.1476-5381.1991.tb12434.x

Renovascular hypertension impairs formation of endothelium-derived relaxing factors and sensitivity to endothelin-1 in resistance arteries.

Y Dohi 1, L Criscione 1, T F Lüscher 1
PMCID: PMC1908557  PMID: 1797302

Abstract

1. Endothelium-dependent vascular regulation was investigated in mesenteric resistance arteries of Goldblatt two kidney-one clip (2K1C) renovascular hypertensive rats. 2. Third order branches of mesenteric arteries were dissected free and mounted on glass cannulae in an organ chamber. Changes in vascular diameter were measured in pressurized and perfused arteries with a video dimension analyzer. 3. Acetylcholine evoked endothelium-dependent relaxations that were much more pronounced with intraluminal than with extraluminal application. In 2K1C rats the relaxation induced by intraluminal, but not extraluminal acetylcholine was decreased compared to normotensive Wistar Kyoto rats (WKY). Increased duration of hypertension further decreased the response to intraluminal but not extraluminal acetylcholine. 4. Endothelin-1 and noradrenaline caused contractions which were augmented by removal of the endothelium. This augmentation was reduced in 2K1C rats compared to WKY; the difference was small with noradrenaline but more pronounced with endothelin-1. 5. In arteries without endothelium the sensitivity, but not the maximal contraction to endothelin-1 was lower in 2K1C rats, while the response to noradrenaline was not different in 2K1C rats and WKY. The sensitivity to the peptide was not further affected by increasing the duration of hypertension. 6. Thus, renovascular hypertension leads to an impaired intraluminal, but not extraluminal activation of the release of endothelium-derived relaxing factor and a decreased inhibitory effect of the endothelium against endothelin-1- and noradrenaline-induced contractions in mesenteric resistance arteries. Furthermore, the sensitivity, but not the maximal response of vascular smooth muscle to endothelin-1 was reduced.

Full text

PDF
349

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUNNER H., DESAULLES P. A., REGOLI D., GROSS F. Renin content and excretory function of the kidney in rats with experimental hypertension. Am J Physiol. 1962 Apr;202:795–799. doi: 10.1152/ajplegacy.1962.202.4.795. [DOI] [PubMed] [Google Scholar]
  2. Cernacek P., Stewart D. J. Immunoreactive endothelin in human plasma: marked elevations in patients in cardiogenic shock. Biochem Biophys Res Commun. 1989 Jun 15;161(2):562–567. doi: 10.1016/0006-291x(89)92636-3. [DOI] [PubMed] [Google Scholar]
  3. Criscione L., Nellis P., Riniker B., Thomann H., Burdet R. Reactivity and sensitivity of mesenteric vascular beds and aortic rings of spontaneously hypertensive rats to endothelin: effects of calcium entry blockers. Br J Pharmacol. 1990 May;100(1):31–36. doi: 10.1111/j.1476-5381.1990.tb12047.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diederich D., Yang Z. H., Bühler F. R., Lüscher T. F. Impaired endothelium-dependent relaxations in hypertensive resistance arteries involve cyclooxygenase pathway. Am J Physiol. 1990 Feb;258(2 Pt 2):H445–H451. doi: 10.1152/ajpheart.1990.258.2.H445. [DOI] [PubMed] [Google Scholar]
  5. Dohi Y., Lüscher T. F. Aging differentially affects direct and indirect actions of endothelin-1 in perfused mesenteric arteries of the rat. Br J Pharmacol. 1990 Aug;100(4):889–893. doi: 10.1111/j.1476-5381.1990.tb14110.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dohi Y., Thiel M. A., Bühler F. R., Lüscher T. F. Activation of endothelial L-arginine pathway in resistance arteries. Effect of age and hypertension. Hypertension. 1990 Aug;16(2):170–179. doi: 10.1161/01.hyp.16.2.170. [DOI] [PubMed] [Google Scholar]
  7. Duarte J., Andrade J., Vargas F. Vascular reactivity in chronic Goldblatt two kidney-one clip hypertensive rats. Experientia. 1990 Aug 15;46(8):868–869. doi: 10.1007/BF01935541. [DOI] [PubMed] [Google Scholar]
  8. Emori T., Hirata Y., Ohta K., Shichiri M., Marumo F. Secretory mechanism of immunoreactive endothelin in cultured bovine endothelial cells. Biochem Biophys Res Commun. 1989 Apr 14;160(1):93–100. doi: 10.1016/0006-291x(89)91625-2. [DOI] [PubMed] [Google Scholar]
  9. Fourcade J. C., Navar L. G., Guyton A. C. Possibility that angiotensin resulting from unilateral kidney disease affects contralateral renal function. Nephron. 1971;8(1):1–16. doi: 10.1159/000179902. [DOI] [PubMed] [Google Scholar]
  10. Halpern W., Osol G., Coy G. S. Mechanical behavior of pressurized in vitro prearteriolar vessels determined with a video system. Ann Biomed Eng. 1984;12(5):463–479. doi: 10.1007/BF02363917. [DOI] [PubMed] [Google Scholar]
  11. Hirata Y., Yoshimi H., Takaichi S., Yanagisawa M., Masaki T. Binding and receptor down-regulation of a novel vasoconstrictor endothelin in cultured rat vascular smooth muscle cells. FEBS Lett. 1988 Oct 24;239(1):13–17. doi: 10.1016/0014-5793(88)80536-2. [DOI] [PubMed] [Google Scholar]
  12. Huang W. C., Ploth D. W., Bell P. D., Work J., Navar L. G. Bilateral renal function responses to converting enzyme inhibitor (SQ 20,881) in two-kidney, one clip Goldblatt hypertensive rats. Hypertension. 1981 May-Jun;3(3):285–293. doi: 10.1161/01.hyp.3.3.285. [DOI] [PubMed] [Google Scholar]
  13. Kohno M., Yasunari K., Murakawa K., Yokokawa K., Horio T., Fukui T., Takeda T. Plasma immunoreactive endothelin in essential hypertension. Am J Med. 1990 Jun;88(6):614–618. doi: 10.1016/0002-9343(90)90527-k. [DOI] [PubMed] [Google Scholar]
  14. Lüscher T. F., Yang Z., Tschudi M., von Segesser L., Stulz P., Boulanger C., Siebenmann R., Turina M., Bühler F. R. Interaction between endothelin-1 and endothelium-derived relaxing factor in human arteries and veins. Circ Res. 1990 Apr;66(4):1088–1094. doi: 10.1161/01.res.66.4.1088. [DOI] [PubMed] [Google Scholar]
  15. Miyauchi T., Ishikawa T., Tomobe Y., Yanagisawa M., Kimura S., Sugishita Y., Ito I., Goto K., Masaki T. Characteristics of pressor response to endothelin in spontaneously hypertensive and Wistar-Kyoto rats. Hypertension. 1989 Oct;14(4):427–434. doi: 10.1161/01.hyp.14.4.427. [DOI] [PubMed] [Google Scholar]
  16. Miyazaki M., Okamura T., Okunishi H., Toda N. Vascular angiotensin converting enzyme in the development of renal hypertension. J Cardiovasc Pharmacol. 1986;8 (Suppl 10):S58–S61. doi: 10.1097/00005344-198600101-00012. [DOI] [PubMed] [Google Scholar]
  17. Ploth D. W., Schnermann J., Dahlheim H., Hermle M., Schmidmeier E. Autoregulation and tubuloglomerular feedback in normotensive and hypertensive rats. Kidney Int. 1977 Oct;12(4):253–267. doi: 10.1038/ki.1977.110. [DOI] [PubMed] [Google Scholar]
  18. Roubert P., Gillard V., Plas P., Chabrier P. E., Braquet P. Down-regulation of endothelin binding sites in rat vascular smooth muscle cells. Am J Hypertens. 1990 Apr;3(4):310–312. doi: 10.1093/ajh/3.4.310. [DOI] [PubMed] [Google Scholar]
  19. Saito Y., Nakao K., Mukoyama M., Imura H. Increased plasma endothelin level in patients with essential hypertension. N Engl J Med. 1990 Jan 18;322(3):205–205. doi: 10.1056/nejm199001183220315. [DOI] [PubMed] [Google Scholar]
  20. Shichiri M., Hirata Y., Ando K., Emori T., Ohta K., Kimoto S., Ogura M., Inoue A., Marumo F. Plasma endothelin levels in hypertension and chronic renal failure. Hypertension. 1990 May;15(5):493–496. doi: 10.1161/01.hyp.15.5.493. [DOI] [PubMed] [Google Scholar]
  21. Suzuki N., Miyauchi T., Tomobe Y., Matsumoto H., Goto K., Masaki T., Fujino M. Plasma concentrations of endothelin-1 in spontaneously hypertensive rats and DOCA-salt hypertensive rats. Biochem Biophys Res Commun. 1990 Mar 30;167(3):941–947. doi: 10.1016/0006-291x(90)90614-s. [DOI] [PubMed] [Google Scholar]
  22. Tesfamariam B., Halpern W. Endothelium-dependent and endothelium-independent vasodilation in resistance arteries from hypertensive rats. Hypertension. 1988 May;11(5):440–444. doi: 10.1161/01.hyp.11.5.440. [DOI] [PubMed] [Google Scholar]
  23. Tomobe Y., Miyauchi T., Saito A., Yanagisawa M., Kimura S., Goto K., Masaki T. Effects of endothelin on the renal artery from spontaneously hypertensive and Wistar Kyoto rats. Eur J Pharmacol. 1988 Aug 2;152(3):373–374. doi: 10.1016/0014-2999(88)90736-4. [DOI] [PubMed] [Google Scholar]
  24. Watt P. A., Thurston H. Endothelium-dependent relaxation in resistance vessels from the spontaneously hypertensive rats. J Hypertens. 1989 Aug;7(8):661–666. doi: 10.1097/00004872-198908000-00010. [DOI] [PubMed] [Google Scholar]
  25. Wilson S. K., Lynch D. R., Ladenson P. W. Angiotensin II and atrial natriuretic factor-binding sites in various tissues in hypertension: comparative receptor localization and changes in different hypertension models in the rat. Endocrinology. 1989 Jun;124(6):2799–2808. doi: 10.1210/endo-124-6-2799. [DOI] [PubMed] [Google Scholar]
  26. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES