Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Oct;104(2):459–465. doi: 10.1111/j.1476-5381.1991.tb12451.x

Adenosine modulation of potassium currents in postganglionic neurones of cultured avian ciliary ganglia.

M R Bennett 1, R Kerr 1, K Nichol 1
PMCID: PMC1908565  PMID: 1797312

Abstract

1. Potassium currents in cultured postganglionic neurones of avian ciliary ganglia were analysed under whole-cell voltage clamp and their modulation by adenosine determined. 2. In the presence of tetrodotoxin (200 nM), and with moderate holding potentials (Vh = -40 mV), the steady-state current-voltage (I/V) curve was N-shaped over the range from -70 mV to +155 mV. CsCl (1 M) blocked the current, indicating that it was carried by K+. If Ca2+ influx was blocked by CdCl2 (500 microM) then the outward current was reduced and the N-shaped I-V curve lost, indicating the presence of a calcium-activated potassium current (IK(Ca)); the remaining current, due to the delayed rectifier (IK), increased with depolarization up to about a conductance of 10 nS near + 50 mV. This IK was 50% activated at about +20 mV and 50% inactivated at about -50 mV. Adenosine (10 microM) had similar affects on the N-shaped I/V curve as did CdCl2, indicating that it blocked IK(Ca). However, adenosine had little affect on the steady-state current in the presence of CdCl, indicating that it did not much affect IK. 3. In the presence of tetrodotoxin (200 nM), a large inward current occurred for large hyperpolarizations from a Vh = -50 mV. This inward rectifying current (IIR) had a reversal potential near EK and showed 50% activation at about -130 mV. Adenosine (10 microM) reduced IIR, by as much as 50% at large hyperpolarizations beyond -80 mV.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
459

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams P. R., Brown D. A., Constanti A. Pharmacological inhibition of the M-current. J Physiol. 1982 Nov;332:223–262. doi: 10.1113/jphysiol.1982.sp014411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett M. R., Ho S. Probabilistic secretion of quanta from nerve terminals in avian ciliary ganglia modulated by adenosine. J Physiol. 1991;440:513–527. doi: 10.1113/jphysiol.1991.sp018722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett M. R., Karunanithi S., Lavidis N. A. Probabilistic secretion of quanta from nerve terminals in toad (Bufo marinus) muscle modulated by adenosine. J Physiol. 1991 Feb;433:421–434. doi: 10.1113/jphysiol.1991.sp018435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown D. A., Adams P. R., Constanti A. Voltage-sensitive K-currents in sympathetic neurons and their modulation by neurotransmitters. J Auton Nerv Syst. 1982 Jul;6(1):23–35. doi: 10.1016/0165-1838(82)90019-4. [DOI] [PubMed] [Google Scholar]
  5. Cassell J. F., Clark A. L., McLachlan E. M. Characteristics of phasic and tonic sympathetic ganglion cells of the guinea-pig. J Physiol. 1986 Mar;372:457–483. doi: 10.1113/jphysiol.1986.sp016020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Constanti A., Galvan M. Fast inward-rectifying current accounts for anomalous rectification in olfactory cortex neurones. J Physiol. 1983 Feb;335:153–178. doi: 10.1113/jphysiol.1983.sp014526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dryer S. E., Fujii J. T., Martin A. R. A Na+-activated K+ current in cultured brain stem neurones from chicks. J Physiol. 1989 Mar;410:283–296. doi: 10.1113/jphysiol.1989.sp017533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fenwick E. M., Marty A., Neher E. A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine. J Physiol. 1982 Oct;331:577–597. doi: 10.1113/jphysiol.1982.sp014393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Galvan M., Sedlmeir C. Outward currents in voltage-clamped rat sympathetic neurones. J Physiol. 1984 Nov;356:115–133. doi: 10.1113/jphysiol.1984.sp015456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardner P. I. Single-channel recordings of three K+-selective currents in cultured chick ciliary ganglion neurons. J Neurosci. 1986 Jul;6(7):2106–2116. doi: 10.1523/JNEUROSCI.06-07-02106.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gerber U., Greene R. W., Haas H. L., Stevens D. R. Characterization of inhibition mediated by adenosine in the hippocampus of the rat in vitro. J Physiol. 1989 Oct;417:567–578. doi: 10.1113/jphysiol.1989.sp017819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  13. Hartung K. Potentiation of a transient outward current by Na+ influx in crayfish neurones. Pflugers Arch. 1985 May;404(1):41–44. doi: 10.1007/BF00581488. [DOI] [PubMed] [Google Scholar]
  14. Hermann A., Gorman A. L. Effects of tetraethylammonium on potassium currents in a molluscan neurons. J Gen Physiol. 1981 Jul;78(1):87–110. doi: 10.1085/jgp.78.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kurachi Y., Nakajima T., Sugimoto T. On the mechanism of activation of muscarinic K+ channels by adenosine in isolated atrial cells: involvement of GTP-binding proteins. Pflugers Arch. 1986 Sep;407(3):264–274. doi: 10.1007/BF00585301. [DOI] [PubMed] [Google Scholar]
  16. Lancaster B., Adams P. R. Calcium-dependent current generating the afterhyperpolarization of hippocampal neurons. J Neurophysiol. 1986 Jun;55(6):1268–1282. doi: 10.1152/jn.1986.55.6.1268. [DOI] [PubMed] [Google Scholar]
  17. Lancaster B., Pennefather P. Potassium currents evoked by brief depolarizations in bull-frog sympathetic ganglion cells. J Physiol. 1987 Jun;387:519–548. doi: 10.1113/jphysiol.1987.sp016587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Latorre R., Oberhauser A., Labarca P., Alvarez O. Varieties of calcium-activated potassium channels. Annu Rev Physiol. 1989;51:385–399. doi: 10.1146/annurev.ph.51.030189.002125. [DOI] [PubMed] [Google Scholar]
  19. Loose M. D., Kelly M. J. Opioids act at mu-receptors to hyperpolarize arcuate neurons via an inwardly rectifying potassium conductance. Brain Res. 1990 Apr 9;513(1):15–23. doi: 10.1016/0006-8993(90)91084-t. [DOI] [PubMed] [Google Scholar]
  20. MARTIN A. R., PILAR G. TRANSMISSION THROUGH THE CILIARY GANGLION OF THE CHICK. J Physiol. 1963 Sep;168:464–475. doi: 10.1113/jphysiol.1963.sp007203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Madison D. V., Nicoll R. A. Actions of noradrenaline recorded intracellularly in rat hippocampal CA1 pyramidal neurones, in vitro. J Physiol. 1986 Mar;372:221–244. doi: 10.1113/jphysiol.1986.sp016006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Phillis J. W., Wu P. H. The role of adenosine and its nucleotides in central synaptic transmission. Prog Neurobiol. 1981;16(3-4):187–239. doi: 10.1016/0301-0082(81)90014-9. [DOI] [PubMed] [Google Scholar]
  23. Segal M., Rogawski M. A., Barker J. L. A transient potassium conductance regulates the excitability of cultured hippocampal and spinal neurons. J Neurosci. 1984 Feb;4(2):604–609. doi: 10.1523/JNEUROSCI.04-02-00604.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Trussell L. O., Jackson M. B. Dependence of an adenosine-activated potassium current on a GTP-binding protein in mammalian central neurons. J Neurosci. 1987 Oct;7(10):3306–3316. doi: 10.1523/JNEUROSCI.07-10-03306.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wong R. K., Prince D. A. Afterpotential generation in hippocampal pyramidal cells. J Neurophysiol. 1981 Jan;45(1):86–97. doi: 10.1152/jn.1981.45.1.86. [DOI] [PubMed] [Google Scholar]
  26. Zbicz K. L., Weight F. F. Transient voltage and calcium-dependent outward currents in hippocampal CA3 pyramidal neurons. J Neurophysiol. 1985 Apr;53(4):1038–1058. doi: 10.1152/jn.1985.53.4.1038. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES