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. 1991 Oct;104(2):445–451. doi: 10.1111/j.1476-5381.1991.tb12449.x

L-glutamate diethyl ester and deaminated analogues as excitatory amino acid antagonists in rat cerebral cortex.

J P Turner 1, B S Meldrum 1
PMCID: PMC1908570  PMID: 1797311

Abstract

1. The effects of L-glutamate diethyl ester (GDEE) HCl, glutarate diethyl ester (GlrDEE) and glutarate dimethyl ester (GlrDME) on depolarizing responses to alpha-amino-3-hydroxy-5- methyl-4-isoxazolepropionate (AMPA), kainate (Kain), N-methyl-D-aspartate (NMDA) and quisqualate (Quis), and spontaneous paroxysmal discharges (SPDs) were examined. Experiments were performed on slices of rat cingulate cortex using the in vitro grease gap recording technique in nominally Mg(2+)-free Krebs medium. 2. GDEE HCl (3-14 mM) caused a concentration-dependent depolarization of the d.c. baseline potential. L-Glutamate (0.1-0.5 mM), HCl (15 mM) and sucrose (30 mM) also depolarized the baseline. GlrDEE (3-12 mM) and GlrDME (4-26 mM) had no consistent effect on baseline potential. 3. GDEE HCl (10 mM) had no effect on depolarizing responses to AMPA, Kain and NMDA, but caused potentiation of those to Quis with a dose-ratio of 0.53 (0.44-0.63) (n = 4). In two other experiments, where the depolarization of the baseline induced by GDEE HCl was large, a depression of Quis response amplitude was observed. 4. GlrDEE (10 mM) antagonized depolarizing responses to Kain, and to a lesser extent NMDA, with dose-ratios of 2.14 (1.92-2.38) and 1.61 (1.39-1.87), respectively. This concentration of GlrDEE had no effect on AMPA responses, but potentiated Quis responses, with a dose-ratio of 0.64 (0.58-0.71). 5. GlrDME (10 mM) antagonized depolarizing responses to Kain and to Quis, with dose-ratios of 1.66 (1.48-1.85) and 1.22 (1.15-1.29), respectively, and had no effect on responses to NMDA.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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