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. 1996 Nov;70(11):7929–7939. doi: 10.1128/jvi.70.11.7929-7939.1996

Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins.

L D Cranmer 1, C L Clark 1, C S Morello 1, H E Farrell 1, W D Rawlinson 1, D H Spector 1
PMCID: PMC190865  PMID: 8892916

Abstract

We have identified three open reading frames (ORFs) in murine cytomegalovirus (MCMV), designated M82, M83, and M84, which likely encode homologs of the human cytomegalovirus (HCMV) UL82 and UL83 matrix phosphoproteins. These ORFs, in the HindIII C fragment of MCMV, are colinear with the UL82, UL83, and UL84 ORFs of HCMV. M82 encodes a 598-amino-acid (aa) protein with homology to UL82, M83 encodes an 809-aa protein with homology to UL82 and UL83, and M84 encodes a 587-aa protein with homology to UL83 and UL84. Analysis of transcription by Northern (RNA) blotting indicated that the M82 and M83 ORFs are transcribed as 2.2- and 5-kb mRNAs, respectively, at 24 to 48 h postinfection (p.i.), while M84 is transcribed as a 6.9-kb mRNA only at 8 h p.i. All transcripts appear to terminate at the same position 3' of the M82 ORF. Of the products of the three ORFs, only M83 is strongly recognized by hyperimmune mouse serum. The M83 protein is a virion-associated phosphoprotein with an apparent molecular mass of 125 kDa. In MCMV-infected cells, it is detectable by Western blotting (immunoblotting) only at 48 h p.i. in the absence of phosphonoacetic acid, consistent with late gene expression. The M83 ORF is also expressed at high levels in cells infected by a recombinant vaccinia virus and yields a protein which is serologically cross-reactive and comigrates with the authentic MCMV protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Selected References

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  1. Beninga J., Kropff B., Mach M. Comparative analysis of fourteen individual human cytomegalovirus proteins for helper T cell response. J Gen Virol. 1995 Jan;76(Pt 1):153–160. doi: 10.1099/0022-1317-76-1-153. [DOI] [PubMed] [Google Scholar]
  2. Biegalke B. J., Geballe A. P. Translational inhibition by cytomegalovirus transcript leaders. Virology. 1990 Aug;177(2):657–667. doi: 10.1016/0042-6822(90)90531-u. [DOI] [PubMed] [Google Scholar]
  3. Borysiewicz L. K., Hickling J. K., Graham S., Sinclair J., Cranage M. P., Smith G. L., Sissons J. G. Human cytomegalovirus-specific cytotoxic T cells. Relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia viruses. J Exp Med. 1988 Sep 1;168(3):919–931. doi: 10.1084/jem.168.3.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Britt W. J. Recent advances in the identification of significant human cytomegalovirus-encoded proteins. Transplant Proc. 1991 Jun;23(3 Suppl 3):64-9, discussion 69. [PubMed] [Google Scholar]
  5. Bruggeman C. A. Cytomegalovirus and latency: an overview. Virchows Arch B Cell Pathol Incl Mol Pathol. 1993;64(6):325–333. doi: 10.1007/BF02915131. [DOI] [PubMed] [Google Scholar]
  6. Chakrabarti S., Brechling K., Moss B. Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985 Dec;5(12):3403–3409. doi: 10.1128/mcb.5.12.3403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., 3rd, Kouzarides T., Martignetti J. A. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. doi: 10.1007/978-3-642-74980-3_6. [DOI] [PubMed] [Google Scholar]
  8. Chee M., Rudolph S. A., Plachter B., Barrell B., Jahn G. Identification of the major capsid protein gene of human cytomegalovirus. J Virol. 1989 Mar;63(3):1345–1353. doi: 10.1128/jvi.63.3.1345-1353.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cranmer L. D., Clark C., Spector D. H. Cloning, characterization, and expression of the murine cytomegalovirus homologue of the human cytomegalovirus 28-kDa matrix phosphoprotein (UL99). Virology. 1994 Dec;205(2):417–429. doi: 10.1006/viro.1994.1662. [DOI] [PubMed] [Google Scholar]
  10. Depto A. S., Stenberg R. M. Regulated expression of the human cytomegalovirus pp65 gene: octamer sequence in the promoter is required for activation by viral gene products. J Virol. 1989 Mar;63(3):1232–1238. doi: 10.1128/jvi.63.3.1232-1238.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Elliott R., Clark C., Jaquish D., Spector D. H. Transcription analysis and sequence of the putative murine cytomegalovirus DNA polymerase gene. Virology. 1991 Nov;185(1):169–186. doi: 10.1016/0042-6822(91)90765-4. [DOI] [PubMed] [Google Scholar]
  12. Feng D. F., Doolittle R. F. Progressive alignment and phylogenetic tree construction of protein sequences. Methods Enzymol. 1990;183:375–387. doi: 10.1016/0076-6879(90)83025-5. [DOI] [PubMed] [Google Scholar]
  13. Feng D. F., Doolittle R. F. Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J Mol Evol. 1987;25(4):351–360. doi: 10.1007/BF02603120. [DOI] [PubMed] [Google Scholar]
  14. Fourney R. M., Dietrich K. D., Aubin R. A., Paterson M. C. HindIII polymorphism in the human c-sis proto-oncogene. Nucleic Acids Res. 1988 Aug 25;16(16):8197–8197. doi: 10.1093/nar/16.16.8197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Geballe A. P., Leach F. S., Mocarski E. S. Regulation of cytomegalovirus late gene expression: gamma genes are controlled by posttranscriptional events. J Virol. 1986 Mar;57(3):864–874. doi: 10.1128/jvi.57.3.864-874.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gilbert M. J., Riddell S. R., Li C. R., Greenberg P. D. Selective interference with class I major histocompatibility complex presentation of the major immediate-early protein following infection with human cytomegalovirus. J Virol. 1993 Jun;67(6):3461–3469. doi: 10.1128/jvi.67.6.3461-3469.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Giugni T. D., Churchill M. A., Pande H., Campo K., Guha M., Zaia J. A. Expression in insect cells and immune reactivity of a 28K tegument protein of human cytomegalovirus. J Gen Virol. 1992 Sep;73(Pt 9):2367–2374. doi: 10.1099/0022-1317-73-9-2367. [DOI] [PubMed] [Google Scholar]
  18. Gompels U. A., Nicholas J., Lawrence G., Jones M., Thomson B. J., Martin M. E., Efstathiou S., Craxton M., Macaulay H. A. The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution. Virology. 1995 May 10;209(1):29–51. doi: 10.1006/viro.1995.1228. [DOI] [PubMed] [Google Scholar]
  19. Greenberg P. D., Reusser P., Goodrich J. M., Riddell S. R. Development of a treatment regimen for human cytomegalovirus (CMV) infection in bone marrow transplantation recipients by adoptive transfer of donor-derived CMV-specific T cell clones expanded in vitro. Ann N Y Acad Sci. 1991 Dec 30;636:184–195. doi: 10.1111/j.1749-6632.1991.tb33450.x. [DOI] [PubMed] [Google Scholar]
  20. Guan K. L., Dixon J. E. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem. 1991 Feb 1;192(2):262–267. doi: 10.1016/0003-2697(91)90534-z. [DOI] [PubMed] [Google Scholar]
  21. He Y. S., Xu L., Huang E. S. Characterization of human cytomegalovirus UL84 early gene and identification of its putative protein product. J Virol. 1992 Feb;66(2):1098–1108. doi: 10.1128/jvi.66.2.1098-1108.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Iskenderian A. C., Huang L., Reilly A., Stenberg R. M., Anders D. G. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J Virol. 1996 Jan;70(1):383–392. doi: 10.1128/jvi.70.1.383-392.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Jahn G., Kouzarides T., Mach M., Scholl B. C., Plachter B., Traupe B., Preddie E., Satchwell S. C., Fleckenstein B., Barrell B. G. Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. J Virol. 1987 May;61(5):1358–1367. doi: 10.1128/jvi.61.5.1358-1367.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Jahn G., Scholl B. C., Traupe B., Fleckenstein B. The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. J Gen Virol. 1987 May;68(Pt 5):1327–1337. doi: 10.1099/0022-1317-68-5-1327. [DOI] [PubMed] [Google Scholar]
  25. Jonjić S., del Val M., Keil G. M., Reddehase M. J., Koszinowski U. H. A nonstructural viral protein expressed by a recombinant vaccinia virus protects against lethal cytomegalovirus infection. J Virol. 1988 May;62(5):1653–1658. doi: 10.1128/jvi.62.5.1653-1658.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Koszinowski U. H., Keil G. M., Schwarz H., Schickedanz J., Reddehase M. J. A nonstructural polypeptide encoded by immediate-early transcription unit 1 of murine cytomegalovirus is recognized by cytolytic T lymphocytes. J Exp Med. 1987 Jul 1;166(1):289–294. doi: 10.1084/jem.166.1.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Koszinowski U. H., Reddehase M. J., Keil G. M., Schickedanz J. Host immune response to cytomegalovirus: products of transfected viral immediate-early genes are recognized by cloned cytolytic T lymphocytes. J Virol. 1987 Jun;61(6):2054–2058. doi: 10.1128/jvi.61.6.2054-2058.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Koszinowski U. H., Reddehase M. J., Keil G. M., Volkmer H., Jonjic S., Messerle M., del Val M., Mutter W., Münch K., Bühler B. Molecular analysis of herpesviral gene products recognized by protective cytolytic T lymphocytes. Immunol Lett. 1987 Dec;16(3-4):185–192. doi: 10.1016/0165-2478(87)90146-5. [DOI] [PubMed] [Google Scholar]
  29. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  30. Landini M. P., Michelson S. Human cytomegalovirus proteins. Prog Med Virol. 1988;35:152–185. [PubMed] [Google Scholar]
  31. Landini M. P., Re M. C., Mirolo G., Baldassarri B., La Placa M. Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol. 1985 Dec;17(4):303–311. doi: 10.1002/jmv.1890170403. [DOI] [PubMed] [Google Scholar]
  32. Landini M. P., Severi B., Furlini G., Badiali De Giorgi L. Human cytomegalovirus structural components: intracellular and intraviral localization of p28 and p65-69 by immunoelectron microscopy. Virus Res. 1987 Jul;8(1):15–23. doi: 10.1016/0168-1702(87)90036-0. [DOI] [PubMed] [Google Scholar]
  33. Lawrence G. L., Chee M., Craxton M. A., Gompels U. A., Honess R. W., Barrell B. G. Human herpesvirus 6 is closely related to human cytomegalovirus. J Virol. 1990 Jan;64(1):287–299. doi: 10.1128/jvi.64.1.287-299.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Leader D. P., Katan M. Viral aspects of protein phosphorylation. J Gen Virol. 1988 Jul;69(Pt 7):1441–1464. doi: 10.1099/0022-1317-69-7-1441. [DOI] [PubMed] [Google Scholar]
  35. McLaughlin-Taylor E., Pande H., Forman S. J., Tanamachi B., Li C. R., Zaia J. A., Greenberg P. D., Riddell S. R. Identification of the major late human cytomegalovirus matrix protein pp65 as a target antigen for CD8+ virus-specific cytotoxic T lymphocytes. J Med Virol. 1994 May;43(1):103–110. doi: 10.1002/jmv.1890430119. [DOI] [PubMed] [Google Scholar]
  36. Mercer J. A., Marks J. R., Spector D. H. Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain). Virology. 1983 Aug;129(1):94–106. doi: 10.1016/0042-6822(83)90398-7. [DOI] [PubMed] [Google Scholar]
  37. Meyer H., Bankier A. T., Landini M. P., Brown C. M., Barrell B. G., Rüger B., Mach M. Identification and procaryotic expression of the gene coding for the highly immunogenic 28-kilodalton structural phosphoprotein (pp28) of human cytomegalovirus. J Virol. 1988 Jul;62(7):2243–2250. doi: 10.1128/jvi.62.7.2243-2250.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Moss B. Regulation of vaccinia virus transcription. Annu Rev Biochem. 1990;59:661–688. doi: 10.1146/annurev.bi.59.070190.003305. [DOI] [PubMed] [Google Scholar]
  39. Moss B. Vaccinia virus: a tool for research and vaccine development. Science. 1991 Jun 21;252(5013):1662–1667. doi: 10.1126/science.2047875. [DOI] [PubMed] [Google Scholar]
  40. Nowak B., Gmeiner A., Sarnow P., Levine A. J., Fleckenstein B. Physical mapping of human cytomegalovirus genes: identification of DNA sequences coding for a virion phosphoprotein of 71 kDa and a viral 65-kDa polypeptide. Virology. 1984 Apr 15;134(1):91–102. doi: 10.1016/0042-6822(84)90275-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Pande H., Campo K., Tanamachi B., Zaia J. A. Human cytomegalovirus strain Towne pp28 gene: sequence comparison to pp28 of HCMV AD169 and stable expression in Chinese hamster ovary cells. Virology. 1991 Oct;184(2):762–767. doi: 10.1016/0042-6822(91)90448-k. [DOI] [PubMed] [Google Scholar]
  42. Pande H., Campo K., Tanamachi B., Zaia J. A. Human cytomegalovirus strain Towne pp65 gene: nucleotide sequence and expression in Escherichia coli. Virology. 1991 May;182(1):220–228. doi: 10.1016/0042-6822(91)90665-x. [DOI] [PubMed] [Google Scholar]
  43. Pande H., Lee T. D., Churchill M. A., Zaia J. A. Structural analysis of a 64-kDa major structural protein of human cytomegalovirus (Towne): identification of a phosphorylation site and comparison to pp65 of HCMV (AD169). Virology. 1990 Sep;178(1):6–14. doi: 10.1016/0042-6822(90)90374-z. [DOI] [PubMed] [Google Scholar]
  44. Pari G. S., Anders D. G. Eleven loci encoding trans-acting factors are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA replication. J Virol. 1993 Dec;67(12):6979–6988. doi: 10.1128/jvi.67.12.6979-6988.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Pearson W. R. Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 1990;183:63–98. doi: 10.1016/0076-6879(90)83007-v. [DOI] [PubMed] [Google Scholar]
  47. Pereira L., Hoffman M., Cremer N. Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera. Infect Immun. 1982 Jun;36(3):933–942. doi: 10.1128/iai.36.3.933-942.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Reddehase M. J., Jonjić S., Weiland F., Mutter W., Koszinowski U. H. Adoptive immunotherapy of murine cytomegalovirus adrenalitis in the immunocompromised host: CD4-helper-independent antiviral function of CD8-positive memory T lymphocytes derived from latently infected donors. J Virol. 1988 Mar;62(3):1061–1065. doi: 10.1128/jvi.62.3.1061-1065.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Reddehase M. J., Keil G. M., Koszinowski U. H. The cytolytic T lymphocyte response to the murine cytomegalovirus. II. Detection of virus replication stage-specific antigens by separate populations of in vivo active cytolytic T lymphocyte precursors. Eur J Immunol. 1984 Jan;14(1):56–61. doi: 10.1002/eji.1830140111. [DOI] [PubMed] [Google Scholar]
  50. Reddehase M. J., Koszinowski U. H. Significance of herpesvirus immediate early gene expression in cellular immunity to cytomegalovirus infection. Nature. 1984 Nov 22;312(5992):369–371. doi: 10.1038/312369a0. [DOI] [PubMed] [Google Scholar]
  51. Reddehase M. J., Mutter W., Koszinowski U. H. In vivo application of recombinant interleukin 2 in the immunotherapy of established cytomegalovirus infection. J Exp Med. 1987 Mar 1;165(3):650–656. doi: 10.1084/jem.165.3.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. Reddehase M. J., Mutter W., Münch K., Bühring H. J., Koszinowski U. H. CD8-positive T lymphocytes specific for murine cytomegalovirus immediate-early antigens mediate protective immunity. J Virol. 1987 Oct;61(10):3102–3108. doi: 10.1128/jvi.61.10.3102-3108.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Reddehase M. J., Weiland F., Münch K., Jonjic S., Lüske A., Koszinowski U. H. Interstitial murine cytomegalovirus pneumonia after irradiation: characterization of cells that limit viral replication during established infection of the lungs. J Virol. 1985 Aug;55(2):264–273. doi: 10.1128/jvi.55.2.264-273.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Riddell S. R., Rabin M., Geballe A. P., Britt W. J., Greenberg P. D. Class I MHC-restricted cytotoxic T lymphocyte recognition of cells infected with human cytomegalovirus does not require endogenous viral gene expression. J Immunol. 1991 Apr 15;146(8):2795–2804. [PubMed] [Google Scholar]
  55. Riddell S. R., Watanabe K. S., Goodrich J. M., Li C. R., Agha M. E., Greenberg P. D. Restoration of viral immunity in immunodeficient humans by the adoptive transfer of T cell clones. Science. 1992 Jul 10;257(5067):238–241. doi: 10.1126/science.1352912. [DOI] [PubMed] [Google Scholar]
  56. Ripalti A., Ruan Q., Boccuni M. C., Campanini F., Bergamini G., Landini M. P. Construction of polyepitope fusion antigens of human cytomegalovirus ppUL32: reactivity with human antibodies. J Clin Microbiol. 1994 Feb;32(2):358–363. doi: 10.1128/jcm.32.2.358-363.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Roby C., Gibson W. Characterization of phosphoproteins and protein kinase activity of virions, noninfectious enveloped particles, and dense bodies of human cytomegalovirus. J Virol. 1986 Sep;59(3):714–727. doi: 10.1128/jvi.59.3.714-727.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Rüger B., Klages S., Walla B., Albrecht J., Fleckenstein B., Tomlinson P., Barrell B. Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus. J Virol. 1987 Feb;61(2):446–453. doi: 10.1128/jvi.61.2.446-453.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  59. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Spector D. H., Hock L., Tamashiro J. C. Cleavage maps for human cytomegalovirus DNA strain AD169 for restriction endonucleases EcoRI, BglII, and HindIII. J Virol. 1982 May;42(2):558–582. doi: 10.1128/jvi.42.2.558-582.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Spector D. J., Tevethia M. J. Protein-protein interactions between human cytomegalovirus IE2-580aa and pUL84 in lytically infected cells. J Virol. 1994 Nov;68(11):7549–7553. doi: 10.1128/jvi.68.11.7549-7553.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  62. Staczek J. Animal cytomegaloviruses. Microbiol Rev. 1990 Sep;54(3):247–265. doi: 10.1128/mr.54.3.247-265.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Tsutsui Y. Developmental disorders of the mouse brain induced by murine cytomegalovirus: animal models for congenital cytomegalovirus infection. Pathol Int. 1995 Feb;45(2):91–102. doi: 10.1111/j.1440-1827.1995.tb03428.x. [DOI] [PubMed] [Google Scholar]
  64. Volkmer H., Bertholet C., Jonjić S., Wittek R., Koszinowski U. H. Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus. J Exp Med. 1987 Sep 1;166(3):668–677. doi: 10.1084/jem.166.3.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  65. Walter E. A., Greenberg P. D., Gilbert M. J., Finch R. J., Watanabe K. S., Thomas E. D., Riddell S. R. Reconstitution of cellular immunity against cytomegalovirus in recipients of allogeneic bone marrow by transfer of T-cell clones from the donor. N Engl J Med. 1995 Oct 19;333(16):1038–1044. doi: 10.1056/NEJM199510193331603. [DOI] [PubMed] [Google Scholar]
  66. Wright D. A., Staprans S. I., Spector D. H. Four phosphoproteins with common amino termini are encoded by human cytomegalovirus AD169. J Virol. 1988 Jan;62(1):331–340. doi: 10.1128/jvi.62.1.331-340.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Zaia J. A., Forman S. J., Ting Y. P., Vanderwal-Urbina E., Blume K. G. Polypeptide-specific antibody response to human cytomegalovirus after infection in bone marrow transplant recipients. J Infect Dis. 1986 Apr;153(4):780–787. doi: 10.1093/infdis/153.4.780. [DOI] [PubMed] [Google Scholar]

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