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. 1992 Feb;105(2):257–258. doi: 10.1111/j.1476-5381.1992.tb14241.x

Low dihydropyridine receptor density in vasa deferentia of castrated rats.

C J Castillo 1, S Lafayette 1, A Caricati-Neto 1, M Sette 1, N H Jurkiewicz 1, A G García 1, A Jurkiewicz 1
PMCID: PMC1908652  PMID: 1313721

Abstract

Radioligand binding studies in crude membrane preparations of vasa deferentia of normal rats, with the 1,4-dihydropyridine (+)-[3H]-PN200-110 (isradipine) showed typical saturation isotherms. The binding exhibited a KD of 259 +/- 60 pM and Bmax of 144 +/- 20 fmol mg-1 protein. The low KD and the stereoselective displacement of (+)-[3H]-PN200-110 binding by (+)- and (-)-PN200-110 and by nifedipine suggests that these tissues contain dihydropyridine receptors probably coupled to voltage-sensitive, L-type calcium channels. In membrane preparations from vasa deferentia from rats castrated 30 days previously the maximum specific binding was 25 +/- 10 fmol mg-1 protein, representing only 11% of total binding; thus, the calculation of reliable KD values was not feasible. These findings suggest that a testicular hormone, possibly testosterone, plays an important role in the regulation of dihydropyridine-sensitive, voltage-dependent calcium channels in the rat vas deferens.

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Selected References

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