Abstract
The effects of BRL 38227 and glibenclamide on intracellular calcium stores were investigated in permeabilized cultured airway smooth muscle cells of the rabbit using 45Ca effluxes. BRL 38227 (10 microM) reduced loading of the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular store by 26.5% +/- 1.0; this effect was antagonized by 1 microM glibenclamide. BRL 38227 itself did not release calcium and had no effect on guanosine 5'-0-(3-thiotriphosphate)-induced calcium release. Glibenclamide (greater than or equal to 5 microM) also reduced calcium loading of the intracellular store, and enhanced calcium release. These results suggest that BRL 38227 has a direct effect on intracellular calcium handling.
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