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. 1992 Feb;105(2):263–270. doi: 10.1111/j.1476-5381.1992.tb14244.x

Evidence for the absence of a functional role for muscarinic M2 inhibitory receptors in cat trachea in vivo: contrast with in vitro results.

C R Killingsworth 1, M F Yu 1, N E Robinson 1
PMCID: PMC1908663  PMID: 1559124

Abstract

1. The effect of the selective muscarinic M2 receptor antagonist, gallamine and the selective M2 receptor agonist, pilocarpine, on airway constriction induced by vagal stimulation was studied in anaesthetized cats. In addition, the effect of gallamine on contraction of cat isolated tracheal and bronchi preparations induced by electrical field stimulation was also investigated. 2. In in vivo experiments, extrathoracic airway constriction was measured with an electromechanical caliper that was attached to the outer surface of tracheal ring 4. Intrathoracic airway constriction was determined by measuring the changes in total lung resistance and dynamic compliance during vagal stimulation. 3. Intravenous gallamine (0.1, 1, and 10 mg kg-1) augmented the rise in total lung resistance induced by vagal stimulation in a dose- and frequency-dependent manner. At stimulation frequencies of 8 and 12 Hz the fall in dynamic compliance provoked by vagal stimulation was also significantly increased by gallamine (10 mg kg-1). Gallamine was without effect on airway constriction induced by acetylcholine. 4. Vagal stimulation at 4 Hz produced significant tracheal constriction, but the amount of constriction did not change following injection of increasing doses of gallamine. Similarly, there was no difference in tracheal constriction at any frequency of stimulation (0.5-16 Hz) when frequency-response curves before and after gallamine injection (10 mg kg-1) were compared. 5. Pilocarpine (0.01-10 micrograms kg-1, i.v.) diminished changes in total lung resistance and dynamic compliance induced by vagal stimulation, an effect that was reversed by gallamine (10 mg kg-1, i.v.).(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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