Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1991 Dec;104(4):776–778. doi: 10.1111/j.1476-5381.1991.tb12505.x

Neuropeptide Y, peptide YY and C-terminal fragments release histamine from rat peritoneal mast cells.

L Grundemar 1, R Håkanson 1
PMCID: PMC1908835  PMID: 1725763

Abstract

1. Neuropeptide Y (NPY) and peptide YY (PYY) seem to act on at least two receptor subtypes, Y1 and Y2. The Y1-receptor requires the whole C-terminally amidated NPY/PYY molecule whereas the Y2-receptor in addition recognizes C-terminal fragments of the two peptides. The present study was designed to elucidate whether NPY and related peptides were able to release histamine from isolated peritoneal mast cells of the rat. 2. NPY, NPY 15-36, NPY 22-36, NPY 26-36 and desamido-NPY evoked a concentration-dependent release of mast-cell histamine. The pEC15 values for NPY 15-36 and NPY 22-36 were higher, while the pEC15 value for NPY 26-36 was lower than that for NPY. At the highest concentration tested (0.1 mM), NPY and its C-terminal fragments released between 30 and 40% of the total histamine content. At the same concentration desamido-NPY released about 20%. 3. PYY and PYY 15-36 also evoked a concentration-dependent release of mast-cell histamine. PYY was more effective than PYY 15-36 since, at 0.1 mM, PYY released about 33%, while PYY 15-36 released about 15% of the total histamine content. Pancreatic polypeptide (PP) and the Y1-receptor-selective agonist [Pro34]NPY were virtually inactive. 4. The effect profile of the NPY/PYY-related peptides suggests that they act on the mast cells by a mechanism that does not involve either of the receptor subtypes hitherto described.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
776

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boublik J. H., Scott N. A., Brown M. R., Rivier J. E. Synthesis and hypertensive activity of neuropeptide Y fragments and analogues with modified N- or C-termini or D-substitutions. J Med Chem. 1989 Mar;32(3):597–601. doi: 10.1021/jm00123a014. [DOI] [PubMed] [Google Scholar]
  2. Boublik J., Scott N., Taulane J., Goodman M., Brown M., Rivier J. Neuropeptide Y and neuropeptide Y18-36. Structural and biological characterization. Int J Pept Protein Res. 1989 Jan;33(1):11–15. doi: 10.1111/j.1399-3011.1989.tb00677.x. [DOI] [PubMed] [Google Scholar]
  3. Böttcher G., Sjölund K., Ekblad E., Håkanson R., Schwartz T. W., Sundler F. Coexistence of peptide YY and glicentin immunoreactivity in endocrine cells of the gut. Regul Pept. 1984 Jul;8(4):261–266. doi: 10.1016/0167-0115(84)90034-x. [DOI] [PubMed] [Google Scholar]
  4. Ekblad E., Edvinsson L., Wahlestedt C., Uddman R., Håkanson R., Sundler F. Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Regul Pept. 1984 Apr;8(3):225–235. doi: 10.1016/0167-0115(84)90064-8. [DOI] [PubMed] [Google Scholar]
  5. Grundemar L., Håkanson R. Effects of various neuropeptide Y/peptide YY fragments on electrically-evoked contractions of the rat vas deferens. Br J Pharmacol. 1990 May;100(1):190–192. doi: 10.1111/j.1476-5381.1990.tb12075.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grundemar L., Wahlestedt C., Shen G. H., Zukowska-Grojec Z., Håkanson R. Biphasic blood pressure response to neuropeptide Y in anesthetized rats. Eur J Pharmacol. 1990 Apr 10;179(1-2):83–87. doi: 10.1016/0014-2999(90)90404-t. [DOI] [PubMed] [Google Scholar]
  7. Håkanson R., Rönnberg A. L. Improved fluorometric assay of histamine: condensation with O-phthalaldehyde at -20 degrees C. Anal Biochem. 1974 Aug;60(2):560–567. doi: 10.1016/0003-2697(74)90267-x. [DOI] [PubMed] [Google Scholar]
  8. Krstenansky J. L., Owen T. J., Payne M. H., Shatzer S. A., Buck S. H. C-terminal modifications of neuropeptide Y and its analogs leading to selectivity for the mouse brain receptor over the porcine spleen receptor. Neuropeptides. 1990 Nov;17(3):117–120. doi: 10.1016/0143-4179(90)90073-8. [DOI] [PubMed] [Google Scholar]
  9. Kurose M., Saeki K. Histamine release induced by neurotensin from rat peritoneal mast cells. Eur J Pharmacol. 1981 Dec 3;76(2-3):129–136. doi: 10.1016/0014-2999(81)90494-5. [DOI] [PubMed] [Google Scholar]
  10. Lundberg J. M., Tatemoto K., Terenius L., Hellström P. M., Mutt V., Hökfelt T., Hamberger B. Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4471–4475. doi: 10.1073/pnas.79.14.4471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lundberg J. M., Terenius L., Hökfelt T., Martling C. R., Tatemoto K., Mutt V., Polak J., Bloom S., Goldstein M. Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand. 1982 Dec;116(4):477–480. doi: 10.1111/j.1748-1716.1982.tb07171.x. [DOI] [PubMed] [Google Scholar]
  12. Mousli M., Bronner C., Bueb J. L., Tschirhart E., Gies J. P., Landry Y. Activation of rat peritoneal mast cells by substance P and mastoparan. J Pharmacol Exp Ther. 1989 Jul;250(1):329–335. [PubMed] [Google Scholar]
  13. Mousli M., Bueb J. L., Bronner C., Rouot B., Landry Y. G protein activation: a receptor-independent mode of action for cationic amphiphilic neuropeptides and venom peptides. Trends Pharmacol Sci. 1990 Sep;11(9):358–362. doi: 10.1016/0165-6147(90)90179-c. [DOI] [PubMed] [Google Scholar]
  14. Nilsson G., Alving K., Ahlstedt S., Hökfelt T., Lundberg J. M. Peptidergic innervation of rat lymphoid tissue and lung: relation to mast cells and sensitivity to capsaicin and immunization. Cell Tissue Res. 1990 Oct;262(1):125–133. doi: 10.1007/BF00327753. [DOI] [PubMed] [Google Scholar]
  15. Rioux F., Bachelard H., Martel J. C., St-Pierre S. The vasoconstrictor effect of neuropeptide Y and related peptides in the guinea pig isolated heart. Peptides. 1986 Jan-Feb;7(1):27–31. doi: 10.1016/0196-9781(86)90056-2. [DOI] [PubMed] [Google Scholar]
  16. Scott N. A., Michel M. C., Boublik J. H., Rivier J. E., Motomura S., Crum R. L., Landon M., Brown M. R. Distinction of NPY receptors in vitro and in vivo. II. Differential effects of NPY and NPY-(18-36). Am J Physiol. 1990 Jul;259(1 Pt 2):H174–H180. doi: 10.1152/ajpheart.1990.259.1.H174. [DOI] [PubMed] [Google Scholar]
  17. Stead R. H., Dixon M. F., Bramwell N. H., Riddell R. H., Bienenstock J. Mast cells are closely apposed to nerves in the human gastrointestinal mucosa. Gastroenterology. 1989 Sep;97(3):575–585. doi: 10.1016/0016-5085(89)90627-6. [DOI] [PubMed] [Google Scholar]
  18. Uddman R., Ekblad E., Edvinsson L., Håkanson R., Sundler F. Neuropeptide Y-like immunoreactivity in perivascular nerve fibres of the guinea-pig. Regul Pept. 1985 Mar;10(2-3):243–257. doi: 10.1016/0167-0115(85)90019-9. [DOI] [PubMed] [Google Scholar]
  19. Wahlestedt C., Grundemar L., Håkanson R., Heilig M., Shen G. H., Zukowska-Grojec Z., Reis D. J. Neuropeptide Y receptor subtypes, Y1 and Y2. Ann N Y Acad Sci. 1990;611:7–26. doi: 10.1111/j.1749-6632.1990.tb48918.x. [DOI] [PubMed] [Google Scholar]
  20. Wallenstein S., Zucker C. L., Fleiss J. L. Some statistical methods useful in circulation research. Circ Res. 1980 Jul;47(1):1–9. doi: 10.1161/01.res.47.1.1. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES