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. 1996 Nov;70(11):8195–8198. doi: 10.1128/jvi.70.11.8195-8198.1996

The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.

J E Wellington 1, G P Allen 1, A A Gooley 1, D N Love 1, N H Packer 1, J X Yan 1, J M Whalley 1
PMCID: PMC190901  PMID: 8892952

Abstract

There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS-PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

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Selected References

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