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. 1995 Dec;116(7):2983–2988. doi: 10.1111/j.1476-5381.1995.tb15953.x

Effects of chronic vitamin E deficiency on vascular function--a study of sympathetic nerves, smooth muscle and endothelium of the mesenteric arterial bed of the rat.

V Ralevic 1, P J Milla 1, G Burnstock 1
PMCID: PMC1909240  PMID: 8680733

Abstract

1. Male rats were deprived as weanlings of dietary vitamin E for 2, 4, 6, 10 and 12 months. Mesenteric arterial beds from these rats and from age-matched controls were isolated and perfused with Krebs solution at a constant flow rate (5 ml min-1). The function of perivascular sympathetic nerves, smooth muscle and endothelium was assessed. 2. At 12 months vitamin E deficient rats exhibited the characteristic symptoms of vitamin E deficiency, namely poor coat condition, muscle wasting, kyphoscoliosis and impaired gait. In the isolated mesenteric arterial bed electrical field stimulation (EFS) of perivascular nerves (4-32 Hz, 90 V, 1 ms, for 30 s) elicited frequency-dependent vasoconstrictor responses which were unaffected by vitamin E deficiency except at 12 months, at which age responses were significantly greater than those of the controls at 24 and 32 Hz (P < 0.01). 3. Exogenous noradrenaline (NA; 0.15-500 nmol) elicited dose-dependent vasoconstriction which was similar in vitamin E-deficient and control preparations at all ages. Potassium chloride (0.15 mmol) also produced similar vasoconstrictor responses in vitamin E-deficient and control preparations at each age. 4. Tone of the preparations was raised by continuous perfusion with methoxamine (4-70 microM), producing similar increases in perfusion pressure in vitamin E-deficient and control preparations at each age. Endothelium-dependent dose-dependent vasodilatation to adenosine 5'-triphosphate was significantly impaired in mesenteric arterial beds from 12 month-old vitamin E-deficient rats compared with the controls (P < 0.05). Relaxation to acetylcholine was not significantly different at any age. 5. Endothelium-independent vasodilatation to sodium nitroprusside was similar in vitamin E-deficient rats and age-matched controls. 6. These results suggest that long term (12 months) deprivation of dietary vitamin E may impair endothelial function in mesenteric arteries of the rat. Sympathetic perivascular nerve constrictor function was increased at 12 months. There were no functionally expressed changes in the vascular smooth muscle, which appears to be more resilient to the effects of oxidative stress in vitamin E deficiency.

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Selected References

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  1. Bieri J. G. Kinetics of tissue -tocopherol depletion and repletion. Ann N Y Acad Sci. 1972 Dec 18;203:181–191. doi: 10.1111/j.1749-6632.1972.tb27874.x. [DOI] [PubMed] [Google Scholar]
  2. Burnstock G. The fifth Heymans memorial lecture-Ghent, February 17, 1990. Co-transmission. Arch Int Pharmacodyn Ther. 1990 Mar-Apr;304:7–33. [PubMed] [Google Scholar]
  3. Burton G. W., Joyce A., Ingold K. U. Is vitamin E the only lipid-soluble, chain-breaking antioxidant in human blood plasma and erythrocyte membranes? Arch Biochem Biophys. 1983 Feb 15;221(1):281–290. doi: 10.1016/0003-9861(83)90145-5. [DOI] [PubMed] [Google Scholar]
  4. Davidge S. T., Hubel C. A., McLaughlin M. K. Cyclooxygenase-dependent vasoconstrictor alters vascular function in the vitamin E-deprived rat. Circ Res. 1993 Jul;73(1):79–88. doi: 10.1161/01.res.73.1.79. [DOI] [PubMed] [Google Scholar]
  5. Esterbauer H., Dieber-Rotheneder M., Waeg G., Striegl G., Jürgens G. Biochemical, structural, and functional properties of oxidized low-density lipoprotein. Chem Res Toxicol. 1990 Mar-Apr;3(2):77–92. doi: 10.1021/tx00014a001. [DOI] [PubMed] [Google Scholar]
  6. Gey K. F., Puska P., Jordan P., Moser U. K. Inverse correlation between plasma vitamin E and mortality from ischemic heart disease in cross-cultural epidemiology. Am J Clin Nutr. 1991 Jan;53(1 Suppl):326S–334S. doi: 10.1093/ajcn/53.1.326S. [DOI] [PubMed] [Google Scholar]
  7. Goss-Sampson M. A., Kriss A., Muller D. P. A longitudinal study of somatosensory, brainstem auditory and peripheral sensory-motor conduction during vitamin E deficiency in the rat. J Neurol Sci. 1990 Dec;100(1-2):79–84. doi: 10.1016/0022-510x(90)90016-g. [DOI] [PubMed] [Google Scholar]
  8. Goss-Sampson M. A., MacEvilly C. J., Muller D. P. Longitudinal studies of the neurobiology of vitamin E and other antioxidant systems, and neurological function in the vitamin E deficient rat. J Neurol Sci. 1988 Oct;87(1):25–35. doi: 10.1016/0022-510x(88)90051-2. [DOI] [PubMed] [Google Scholar]
  9. Gryglewski R. J., Palmer R. M., Moncada S. Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature. 1986 Apr 3;320(6061):454–456. doi: 10.1038/320454a0. [DOI] [PubMed] [Google Scholar]
  10. Halliwell B., Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr. 1993 May;57(5 Suppl):715S–725S. doi: 10.1093/ajcn/57.5.715S. [DOI] [PubMed] [Google Scholar]
  11. Harding A. E. Vitamin E and the nervous system. Crit Rev Neurobiol. 1987;3(1):89–103. [PubMed] [Google Scholar]
  12. Hoyle C. H., Ralevic V., Lincoln J., Knight G. E., Goss-Sampson M. A., Milla P. J., Burnstock G. Effects of vitamin E deficiency on autonomic neuroeffector mechanisms in the rat caecum, vas deferens and urinary bladder. J Physiol. 1995 Sep 15;487(Pt 3):773–786. doi: 10.1113/jphysiol.1995.sp020917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hubel C. A., Griggs K. C., McLaughlin M. K. Lipid peroxidation and altered vascular function in vitamin E-deficient rats. Am J Physiol. 1989 Jun;256(6 Pt 2):H1539–H1545. doi: 10.1152/ajpheart.1989.256.6.H1539. [DOI] [PubMed] [Google Scholar]
  14. Jacobson E. D. Physiology of the mesenteric circulation. Physiologist. 1982 Oct;25(5):439–443. [PubMed] [Google Scholar]
  15. Kaneko T., Nakano S., Matsuo M. Protective effect of vitamin E on linoleic acid hydroperoxide-induced injury to human endothelial cells. Lipids. 1991 May;26(5):345–348. doi: 10.1007/BF02537196. [DOI] [PubMed] [Google Scholar]
  16. MCGREGOR D. D. THE EFFECT OF SYMPATHETIC NERVE STIMULATION OF VASOCONSTRICTOR RESPONSES IN PERFUSED MESENTERIC BLOOD VESSELS OF THE RAT. J Physiol. 1965 Mar;177:21–30. doi: 10.1113/jphysiol.1965.sp007572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Muller D. P., Goss-Sampson M. A. Neurochemical, neurophysiological, and neuropathological studies in vitamin E deficiency. Crit Rev Neurobiol. 1990;5(3):239–263. [PubMed] [Google Scholar]
  18. Olson J. A., Kobayashi S. Antioxidants in health and disease: overview. Proc Soc Exp Biol Med. 1992 Jun;200(2):245–247. doi: 10.3181/00379727-200-43428. [DOI] [PubMed] [Google Scholar]
  19. Packer L. Interactions among antioxidants in health and disease: vitamin E and its redox cycle. Proc Soc Exp Biol Med. 1992 Jun;200(2):271–276. doi: 10.3181/00379727-200-43433. [DOI] [PubMed] [Google Scholar]
  20. Ralevic V., Rubino A., Burnstock G. Prejunctional modulation of sensory-motor nerve-mediated vasodilatation of the rat mesenteric arterial bed by opioid peptides. J Pharmacol Exp Ther. 1994 Feb;268(2):772–778. [PubMed] [Google Scholar]
  21. Rice-Evans C., Burdon R. Free radical-lipid interactions and their pathological consequences. Prog Lipid Res. 1993;32(1):71–110. doi: 10.1016/0163-7827(93)90006-i. [DOI] [PubMed] [Google Scholar]
  22. Rubanyi G. M., Vanhoutte P. M. Superoxide anions and hyperoxia inactivate endothelium-derived relaxing factor. Am J Physiol. 1986 May;250(5 Pt 2):H822–H827. doi: 10.1152/ajpheart.1986.250.5.H822. [DOI] [PubMed] [Google Scholar]
  23. Rubino A., Burnstock G. Recovery after dietary vitamin E supplementation of impaired endothelial function in vitamin E-deficient rats. Br J Pharmacol. 1994 Jun;112(2):515–518. doi: 10.1111/j.1476-5381.1994.tb13103.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schmidt R. E., Coleman B. D., Nelson J. S. Differential effect of chronic vitamin E deficiency on the development of neuroaxonal dystrophy in rat gracile/cuneate nuclei and prevertebral sympathetic ganglia. Neurosci Lett. 1991 Feb 11;123(1):102–106. doi: 10.1016/0304-3940(91)90168-s. [DOI] [PubMed] [Google Scholar]
  25. Simonoff M., Sergeant C., Garnier N., Moretto P., Llabador Y., Simonoff G., Conri C. Antioxidant status (selenium, vitamins A and E) and aging. EXS. 1992;62:368–397. doi: 10.1007/978-3-0348-7460-1_37. [DOI] [PubMed] [Google Scholar]
  26. Sokol R. J. Vitamin E and neurologic deficits. Adv Pediatr. 1990;37:119–148. [PubMed] [Google Scholar]
  27. Towfighi J. Effects of chronic vitamin E deficiency on the nervous system of the rat. Acta Neuropathol. 1981;54(4):261–267. doi: 10.1007/BF00696998. [DOI] [PubMed] [Google Scholar]
  28. van Acker S. A., Koymans L. M., Bast A. Molecular pharmacology of vitamin E: structural aspects of antioxidant activity. Free Radic Biol Med. 1993 Sep;15(3):311–328. doi: 10.1016/0891-5849(93)90078-9. [DOI] [PubMed] [Google Scholar]

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