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LH1 gene copy number in SAOS-2 cells and human liver cells
A 6% polyacrylamide gel showing multiplex-PCR products of the LH1 gene (PLOD1) and type X collagen gene. The relative abundance of PLOD1 and type X collagen PCR products was similar in SAOS-2 DNA and in normal fetal liver DNA, indicating a normal LH1 copy number in SAOS-2 cells. Thus, the high levels of LH1 mRNA are due to elevated expression and/or stability of these products in SAOS-2 cells.
