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. 1996 Aug;118(8):1987–1994. doi: 10.1111/j.1476-5381.1996.tb15634.x

Development of cholinergic and inhibitory non-adrenergic non-cholinergic responses in the rat gastric funds.

G J Smits 1, R A Lefebvre 1
PMCID: PMC1909889  PMID: 8864533

Abstract

1. Cholinergic contractions and inhibitory non-adrenergic non-cholinergic (NANC) relaxations were studied in longitudinal muscle strips of the gastric funds of 2, 4 and 8 week old rats. 2. Contractions induced by electrical stimulation of the cholinergic neurones and by administration of acetylcholine decreased during development. The potentiating effect of physostigmine was similar in the 3 age groups. 3. Short train stimulation in NANC conditions induced fast relaxations, which were more pronounced in 4 and 8 week than in 2 week old rats. These relaxations were almost completely inhibited by NG-nitro-L-arginine methyl ester (L-NAME) in the 3 age groups. The nitric oxide-induced relaxations did not change during development. 4. Sustained electrical stimulation in NANC conditions induced an initial relaxation, which was almost totally blocked by L-NAME, followed by an almost complete recovery of tone at lower frequencies of stimulation. At higher frequencies of stimulation, the recovery of tone was incomplete or absent. This sustained relaxation was only partially reduced by L-NAME and almost abolished by L-NAME plus alpha-chymotrypsin. The initial relaxations increased during development, while the sustained relaxations remained similar during this period. Vasoactive intestinal polypeptide-induced relaxations were also similar in the 3 age groups. 5. These results show that the sensitivity of the gastric fundus to acetylcholine decreases from 2 weeks to 8 weeks postnatally, while the importance of the nitrergic innervation increases during this period.

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1987

Selected References

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  1. Belai A., Cooper S., Burnstock G. Effect of age on NADPH-diaphorase-containing myenteric neurones of rat ileum and proximal colon. Cell Tissue Res. 1995 Feb;279(2):379–383. doi: 10.1007/BF00318495. [DOI] [PubMed] [Google Scholar]
  2. Boeckxstaens G. E., Pelckmans P. A., Bogers J. J., Bult H., De Man J. G., Oosterbosch L., Herman A. G., Van Maercke Y. M. Release of nitric oxide upon stimulation of nonadrenergic noncholinergic nerves in the rat gastric fundus. J Pharmacol Exp Ther. 1991 Feb;256(2):441–447. [PubMed] [Google Scholar]
  3. Boeckxstaens G. E., Pelckmans P. A., De Man J. G., Bult H., Herman A. G., Van Maercke Y. M. Evidence for a differential release of nitric oxide and vasoactive intestinal polypeptide by nonadrenergic noncholinergic nerves in the rat gastric fundus. Arch Int Pharmacodyn Ther. 1992 Jul-Aug;318:107–115. [PubMed] [Google Scholar]
  4. D'Amato M., Currò D., Montuschi P., Ciabattoni G., Ragazzoni E., Lefebvre R. A. Release of vasoactive intestinal polypeptide from the rat gastric fundus. Br J Pharmacol. 1992 Mar;105(3):691–695. doi: 10.1111/j.1476-5381.1992.tb09040.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. D'Amato M., Currò D., Montuschi P. Evidence for dual components in the non-adrenergic non-cholinergic relaxation in the rat gastric fundus: role of endogenous nitric oxide and vasoactive intestinal polypeptide. J Auton Nerv Syst. 1992 Mar;37(3):175–186. doi: 10.1016/0165-1838(92)90039-j. [DOI] [PubMed] [Google Scholar]
  6. De Beurme F. A., Lefebvre R. A. Influence of alpha-chymotrypsin and trypsin on the non-adrenergic non-cholinergic relaxation in the rat gastric fundus. Br J Pharmacol. 1987 May;91(1):171–177. doi: 10.1111/j.1476-5381.1987.tb08996.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furukawa K., Nomoto T. Postnatal changes in response to adenosine and adenine nucleotides in rat duodenum. Br J Pharmacol. 1989 Aug;97(4):1111–1118. doi: 10.1111/j.1476-5381.1989.tb12568.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henning S. J. Postnatal development: coordination of feeding, digestion, and metabolism. Am J Physiol. 1981 Sep;241(3):G199–G214. doi: 10.1152/ajpgi.1981.241.3.G199. [DOI] [PubMed] [Google Scholar]
  9. Herlihy J. T., Murphy R. A. Length-tension relationship of smooth muscle of the hog carotid artery. Circ Res. 1973 Sep;33(3):275–283. doi: 10.1161/01.res.33.3.275. [DOI] [PubMed] [Google Scholar]
  10. Hyland L., Warnock P., Docherty J. R. Age-related alterations in alpha 1- and beta-adrenoceptors mediated responsiveness of rat aorta. Naunyn Schmiedebergs Arch Pharmacol. 1987 Jan;335(1):50–53. doi: 10.1007/BF00165035. [DOI] [PubMed] [Google Scholar]
  11. Irie K., Furukawa K., Nomoto T., Fujii E., Muraki T. Developmental changes in the response of rat isolated duodenum to nicotine. Eur J Pharmacol. 1994 Jan 4;251(1):75–81. doi: 10.1016/0014-2999(94)90445-6. [DOI] [PubMed] [Google Scholar]
  12. Ito S., Kimura A., Ohga A. Development of non-cholinergic, non-adrenergic excitatory and inhibitory responses to intramural nerve stimulation in rat stomach. Br J Pharmacol. 1988 Mar;93(3):684–692. doi: 10.1111/j.1476-5381.1988.tb10327.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelm M., Schrader J. Control of coronary vascular tone by nitric oxide. Circ Res. 1990 Jun;66(6):1561–1575. doi: 10.1161/01.res.66.6.1561. [DOI] [PubMed] [Google Scholar]
  14. Kobashi Y. L., Breuing E. P., Markus R. P. Age-related changes in the reactivity of the rat jejunum to cholinoceptor agonists. Eur J Pharmacol. 1985 Sep 24;115(2-3):133–138. doi: 10.1016/0014-2999(85)90683-1. [DOI] [PubMed] [Google Scholar]
  15. Kojima M., Ishima T., Taniguchi N., Kimura K., Sada H., Sperelakis N. Developmental changes in beta-adrenoceptors, muscarinic cholinoceptors and Ca2+ channels in rat ventricular muscles. Br J Pharmacol. 1990 Feb;99(2):334–339. doi: 10.1111/j.1476-5381.1990.tb14704.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lefebvre R. A., Blancquaert J. P., Willems J. L., Bogaert M. G. In vitro study of the inhibitory effects of dopamine on the rat gastric fundus. Naunyn Schmiedebergs Arch Pharmacol. 1983 Apr;322(3):228–236. doi: 10.1007/BF00500770. [DOI] [PubMed] [Google Scholar]
  17. Lefebvre R. A., De Vriese A., Smits G. J. Influence of vasoactive intestinal polypeptide and NG-nitro-L-arginine methyl ester on cholinergic neurotransmission in the rat gastric fundus. Eur J Pharmacol. 1992 Oct 20;221(2-3):235–242. doi: 10.1016/0014-2999(92)90707-b. [DOI] [PubMed] [Google Scholar]
  18. Lefebvre R. A. Non-adrenergic non-cholinergic neurotransmission in the proximal stomach. Gen Pharmacol. 1993 Mar;24(2):257–266. doi: 10.1016/0306-3623(93)90301-d. [DOI] [PubMed] [Google Scholar]
  19. Lefebvre R. A. Study on the possible neurotransmitter of the non-adrenergic non-cholinergic innervation of the rat gastric fundus. Arch Int Pharmacodyn Ther. 1986 Apr;280(2 Suppl):110–136. [PubMed] [Google Scholar]
  20. Li C. G., Rand M. J. Nitric oxide and vasoactive intestinal polypeptide mediate non-adrenergic, non-cholinergic inhibitory transmission to smooth muscle of the rat gastric fundus. Eur J Pharmacol. 1990 Dec 4;191(3):303–309. doi: 10.1016/0014-2999(90)94162-q. [DOI] [PubMed] [Google Scholar]
  21. Ludbrook J. On making multiple comparisons in clinical and experimental pharmacology and physiology. Clin Exp Pharmacol Physiol. 1991 Jun;18(6):379–392. doi: 10.1111/j.1440-1681.1991.tb01468.x. [DOI] [PubMed] [Google Scholar]
  22. Miyazaki H., Taneike T., Ohga A. Development of cholinergic nerve transmission in the chick oesophagus. Br J Pharmacol. 1989 Mar;96(3):623–630. doi: 10.1111/j.1476-5381.1989.tb11861.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mulhern M., Docherty J. R. Effects of experimental diabetes on the responsiveness of rat aorta. Br J Pharmacol. 1989 Aug;97(4):1007–1012. doi: 10.1111/j.1476-5381.1989.tb12555.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Paul D. A., Ierardi J. A., Parkman H. P., Ryan J. P. Developmental changes in gastric fundus smooth muscle contractility and involvement of extracellular calcium in fetal and adult guinea pigs. Pediatr Res. 1994 Nov;36(5):642–646. doi: 10.1203/00006450-199411000-00019. [DOI] [PubMed] [Google Scholar]
  25. Pulerà N., Bernard P., Carrara M., Bencini C., Pacifici G. M. Muscarinic cholinergic receptors in lung of developing rats. Dev Pharmacol Ther. 1988;11(3):142–146. doi: 10.1159/000457681. [DOI] [PubMed] [Google Scholar]
  26. Smits G. J., Lefebvre R. A. Influence of age on responsiveness of rat gastric fundus to agonists and to stimulation of intrinsic nerves. Eur J Pharmacol. 1992 Nov 13;223(1):97–102. doi: 10.1016/0014-2999(92)90823-m. [DOI] [PubMed] [Google Scholar]
  27. Smits G. J., Lefebvre R. A. Influence of age on the signal transduction pathway of non-adrenergic non-cholinergic neurotransmitters in the rat gastric fundus. Br J Pharmacol. 1995 Feb;114(3):640–647. doi: 10.1111/j.1476-5381.1995.tb17187.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tomomasa T., Hyman P. E., Hsu C. T., Jing J., Snape W. J., Jr Development of the muscarinic receptor in rabbit gastric smooth muscle. Am J Physiol. 1988 May;254(5 Pt 1):G680–G686. doi: 10.1152/ajpgi.1988.254.5.G680. [DOI] [PubMed] [Google Scholar]
  29. VANE J. R. A sensitive method for the assay of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1957 Sep;12(3):344–349. doi: 10.1111/j.1476-5381.1957.tb00146.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zitterman J., Ryan J. P. Development of gastric antral smooth muscle contractility in newborn rabbits. Am J Physiol. 1990 Apr;258(4 Pt 1):G571–G575. doi: 10.1152/ajpgi.1990.258.4.G571. [DOI] [PubMed] [Google Scholar]

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