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. 1996 Aug;118(8):2067–2072. doi: 10.1111/j.1476-5381.1996.tb15645.x

Sources of calcium and alpha 1-adrenoceptor-mediated contraction in rat tail artery.

A Tabernero 1, N M Vivas 1, E Vila 1
PMCID: PMC1909913  PMID: 8864544

Abstract

1. The relative importance of intracellular and extracellular Ca2+ on alpha 1-adrenoceptor-mediated contraction by noradrenaline and St-587 has been studied and correlated with the binding characteristics in intact tail artery from Sprague-Dawley rats. 2. Noradrenaline and St-587 behaved as full agonists inducing a concentration-dependent vasoconstriction. 3. Nifedipine (1 microM and 10 microM) blocked by 50% (P < 0.001) and 75% (P < 0.001) respectively, the maximum contraction (Emax) induced by St-587. Nevertheless, to reach 40% inhibition of Emax on noradrenaline responses (P < 0.01), 10 microM nifedipine was necessary. 4. Both agonists induced a concentration-dependent accumulation of inositol phosphates. Noradrenaline behaved as a full agonist and St-587 as a partial agonist for this response. 5. [3H]-prazosin binding to intact tail artery rings was saturable and of high affinity (KD = 4.44 +/- 0.46 nM; Bmax = 36.35 +/- 4.22 fmol mg-1 tissue). 6. Competition curves for inhibition of specific [3H]-prazosin binding by WB-4101 suggest that the rat tail artery contains two alpha 1-adrenoceptor subtypes in an approximate ratio of 60:40. 7. After irreversible alkylation of alpha 1B-adrenoceptors with 100 microM chloroethylclonidine (CEC), nifedipine (1 microM) influenced to a greater extent the St-587- than the noradrenaline-induced contraction. 8. Our results indicate that the degree of participation of intracellular and extracellular Ca2+ sources, on the alpha 1-adrenoceptor-mediated contraction, depends on the agonist used. The two alpha 1-adrenoceptor subtypes observed in binding experiments seem to be unrelated to the Ca2+ sources used for contraction.

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Selected References

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  1. Alexander P. B., Cheung D. W. Ca2+ mobilization by caffeine in single smooth muscle cells of the rat tail artery. Eur J Pharmacol. 1994 Dec 15;288(1):79–88. doi: 10.1016/0922-4106(94)90012-4. [DOI] [PubMed] [Google Scholar]
  2. Atkinson J., Trescases N., Benedek C., Boillat N., Fouda A. K., Krause F., Pitton M. C., Rafizadeh C., de Rivaz J. C., Sautel M. Alpha-1 and alpha-2 adrenoceptor agonists induce vasoconstriction of the normotensive rat caudal artery in vitro by stimulation of a heterogeneous population of alpha-1 adrenoceptors. Naunyn Schmiedebergs Arch Pharmacol. 1988 Nov;338(5):529–535. doi: 10.1007/BF00179325. [DOI] [PubMed] [Google Scholar]
  3. Badia A., Sallés J. Effects of St-587 on the alpha-adrenoceptors in the bisected rat vas deferens. J Pharm Pharmacol. 1989 Sep;41(9):612–616. doi: 10.1111/j.2042-7158.1989.tb06541.x. [DOI] [PubMed] [Google Scholar]
  4. Beckeringh J. J., Thoolen M. J., De Jonge A., Wilffert B., Timmermans P. B., Van Zwieten P. A. The contractions induced in rat and guinea-pig aortic strips by the alpha 2-adrenoceptor selective agonists B-HT 920 and UK 14,304 are mediated by alpha 1-adrenoceptors. Eur J Pharmacol. 1984 Sep 17;104(3-4):197–203. doi: 10.1016/0014-2999(84)90394-7. [DOI] [PubMed] [Google Scholar]
  5. Berridge M. J., Downes C. P., Hanley M. R. Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands. Biochem J. 1982 Sep 15;206(3):587–595. doi: 10.1042/bj2060587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cauvin C., Malik S. Induction of Ca++ influx and intracellular Ca++ release in isolated rat aorta and mesenteric resistance vessels by norepinephrine activation of alpha-1 receptors. J Pharmacol Exp Ther. 1984 Aug;230(2):413–418. [PubMed] [Google Scholar]
  7. Chiu A. T., Bozarth J. M., Timmermans P. B. Relationship between phosphatidylinositol turnover and Ca++ mobilization induced by alpha-1 adrenoceptor stimulation in the rat aorta. J Pharmacol Exp Ther. 1987 Jan;240(1):123–127. [PubMed] [Google Scholar]
  8. Chiu A. T., McCall D. E., Thoolen M. J., Timmermans P. B. Ca++ utilization in the constriction of rat aorta to full and partial alpha-1 adrenoceptor agonists. J Pharmacol Exp Ther. 1986 Jul;238(1):224–231. [PubMed] [Google Scholar]
  9. Fox A. W., Abel P. W., Minneman K. P. Activation of alpha 1-adrenoceptors increases [3H]inositol metabolism in rat vas deferens and caudal artery. Eur J Pharmacol. 1985 Oct 8;116(1-2):145–152. doi: 10.1016/0014-2999(85)90195-5. [DOI] [PubMed] [Google Scholar]
  10. Godfraind T. Actions of nifedipine on calcium fluxes and contraction in isolated rat arteries. J Pharmacol Exp Ther. 1983 Feb;224(2):443–450. [PubMed] [Google Scholar]
  11. Han C., Abel P. W., Minneman K. P. Alpha 1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle. Nature. 1987 Sep 24;329(6137):333–335. doi: 10.1038/329333a0. [DOI] [PubMed] [Google Scholar]
  12. Hieble J. P., Bylund D. B., Clarke D. E., Eikenburg D. C., Langer S. Z., Lefkowitz R. J., Minneman K. P., Ruffolo R. R., Jr International Union of Pharmacology. X. Recommendation for nomenclature of alpha 1-adrenoceptors: consensus update. Pharmacol Rev. 1995 Jun;47(2):267–270. [PubMed] [Google Scholar]
  13. Labelle E. F., Murray B. M. Differences in inositol phosphate production in rat tail artery and thoracic aorta. J Cell Physiol. 1990 Sep;144(3):391–400. doi: 10.1002/jcp.1041440305. [DOI] [PubMed] [Google Scholar]
  14. Lepretre N., Mironneau J., Arnaudeau S., Tanfin Z., Harbon S., Guillon G., Ibarrondo J. Activation of alpha-1A adrenoceptors mobilizes calcium from the intracellular stores in myocytes from rat portal vein. J Pharmacol Exp Ther. 1994 Jan;268(1):167–174. [PubMed] [Google Scholar]
  15. McPherson G. A. A practical computer-based approach to the analysis of radioligand binding experiments. Comput Programs Biomed. 1983 Aug-Oct;17(1-2):107–113. doi: 10.1016/0010-468x(83)90031-4. [DOI] [PubMed] [Google Scholar]
  16. Michel M. C., Hanft G., Gross G. Alpha 1 B- but not alpha 1 A-adrenoceptors mediate inositol phosphate generation. Naunyn Schmiedebergs Arch Pharmacol. 1990 Apr;341(4):385–387. doi: 10.1007/BF00180666. [DOI] [PubMed] [Google Scholar]
  17. Michel M. C., Kenny B., Schwinn D. A. Classification of alpha 1-adrenoceptor subtypes. Naunyn Schmiedebergs Arch Pharmacol. 1995 Jul;352(1):1–10. doi: 10.1007/BF00169183. [DOI] [PubMed] [Google Scholar]
  18. Minneman K. P., Esbenshade T. A. Alpha 1-adrenergic receptor subtypes. Annu Rev Pharmacol Toxicol. 1994;34:117–133. doi: 10.1146/annurev.pa.34.040194.001001. [DOI] [PubMed] [Google Scholar]
  19. Minneman K. P., Han C., Abel P. W. Comparison of alpha 1-adrenergic receptor subtypes distinguished by chlorethylclonidine and WB 4101. Mol Pharmacol. 1988 May;33(5):509–514. [PubMed] [Google Scholar]
  20. Morel N., Godfraind T. Characterization in rat aorta of the binding sites responsible for blockade of noradrenaline-evoked calcium entry by nisoldipine. Br J Pharmacol. 1991 Feb;102(2):467–477. doi: 10.1111/j.1476-5381.1991.tb12196.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morrow A. L., Creese I. Characterization of alpha 1-adrenergic receptor subtypes in rat brain: a reevaluation of [3H]WB4104 and [3H]prazosin binding. Mol Pharmacol. 1986 Apr;29(4):321–330. [PubMed] [Google Scholar]
  22. Nahorski S. R., Wilcox R. A., Mackrill J. J., Challiss R. A. Phosphoinositide-derived second messengers and the regulation of Ca2+ in vascular smooth muscle. J Hypertens Suppl. 1994 Dec;12(10):S133–S143. [PubMed] [Google Scholar]
  23. Pacaud P., Loirand G., Bolton T. B., Mironneau C., Mironneau J. Intracellular cations modulate noradrenaline-stimulated calcium entry into smooth muscle cells of rat portal vein. J Physiol. 1992 Oct;456:541–556. doi: 10.1113/jphysiol.1992.sp019352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Redfern W. S., MacLean M. R., Clague R. U., McGrath J. C. The role of alpha 2-adrenoceptors in the vasculature of the rat tail. Br J Pharmacol. 1995 Apr;114(8):1724–1730. doi: 10.1111/j.1476-5381.1995.tb14963.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ruffolo R. R., Jr, Hieble J. P. Alpha-adrenoceptors. Pharmacol Ther. 1994;61(1-2):1–64. doi: 10.1016/0163-7258(94)90058-2. [DOI] [PubMed] [Google Scholar]
  26. Sulpizio A., Hieble J. P. Lack of a pharmacological distinction between alpha-1 adrenoceptors mediating intracellular calcium-dependent and independent contractions to sympathetic nerve stimulation in the perfused rat caudal artery. J Pharmacol Exp Ther. 1991 Jun;257(3):1045–1052. [PubMed] [Google Scholar]
  27. Tabernero A., Giraldo J., Vila E. Effect of NG-nitro-L-arginine methylester (L-NAME) on functional and biochemical alpha 1-adrenoceptor-mediated responses in rat blood vessels. Br J Pharmacol. 1996 Feb;117(4):757–763. doi: 10.1111/j.1476-5381.1996.tb15255.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tsai H., Buchholz J., Duckles S. P. Postjunctional alpha 2-adrenoceptors in blood vessels: effect of age. Eur J Pharmacol. 1993 Jun 24;237(2-3):311–316. doi: 10.1016/0014-2999(93)90283-n. [DOI] [PubMed] [Google Scholar]
  29. Vila E., Tabernero A., Ivorra M. D. Inositol phosphate formation and contractile response linked to alpha 1-adrenoceptor in tail artery and aorta from spontaneously hypertensive and Wistar-Kyoto rats. J Cardiovasc Pharmacol. 1993 Aug;22(2):191–197. doi: 10.1097/00005344-199308000-00003. [DOI] [PubMed] [Google Scholar]
  30. Vila E., Thoolen M. J., Beckeringh J. J., Timmermans P. B., Van Zwieten P. A. Lack of effect of D600 on alpha 1-adrenoceptor-mediated contractions of rat isolated anococcygeus muscle. Eur J Pharmacol. 1984 Oct 30;106(1):97–105. doi: 10.1016/0014-2999(84)90682-4. [DOI] [PubMed] [Google Scholar]
  31. Weiss R. J., Webb R. C., Smith C. B. Alpha-2 adrenoreceptors on arterial smooth muscle: selective labeling by [3H]clonidine. J Pharmacol Exp Ther. 1983 Jun;225(3):599–605. [PubMed] [Google Scholar]

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