Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1996 Dec;70(12):9008–9012. doi: 10.1128/jvi.70.12.9008-9012.1996

Inhibition of generation of authentic genomic termini of herpes simplex virus type 1 DNA in temperature-sensitive mutant BHK-21 cells with a mutated CCG1/TAF(II)250 gene.

K Umene 1, T Nishimoto 1
PMCID: PMC191001  PMID: 8971033

Abstract

A temperature-sensitive (ts) mutant from the BHK-21 hamster cell line, tsBN462, has a defect in progression of the G1 phase at the nonpermissive temperature of 39.5 degrees C. The ts mutation in tsBN462 is located in the CCG1 gene, encoding the general transcription factor TAF(II)250. In tsBN462 at 39.5 degrees C, infectious progeny of herpes simplex virus type 1 (HSV-1) was not produced and generation of authentic genomic termini of HSV-1 was inhibited. HSV-1 concatemers containing L components in two possible orientations were produced in tsBN462 at 39.5 degrees C; hence, the generation of authentic genomic termini seemed to be dispensable for inversion of the L component. As production of mRNAs of HSV-1 genes of three kinetic classes in the tsBN462 at 39.5 degrees C was comparable to findings under permissive conditions, the sequential and regulated manner in which HSV-1 gene expression is processed is likely to be maintained in the nonpermissive condition.

Full Text

The Full Text of this article is available as a PDF (265.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Addison C., Rixon F. J., Preston V. G. Herpes simplex virus type 1 UL28 gene product is important for the formation of mature capsids. J Gen Virol. 1990 Oct;71(Pt 10):2377–2384. doi: 10.1099/0022-1317-71-10-2377. [DOI] [PubMed] [Google Scholar]
  2. Baines J. D., Poon A. P., Rovnak J., Roizman B. The herpes simplex virus 1 UL15 gene encodes two proteins and is required for cleavage of genomic viral DNA. J Virol. 1994 Dec;68(12):8118–8124. doi: 10.1128/jvi.68.12.8118-8124.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bataille D., Epstein A. Herpes simplex virus replicative concatemers contain L components in inverted orientation. Virology. 1994 Sep;203(2):384–388. doi: 10.1006/viro.1994.1498. [DOI] [PubMed] [Google Scholar]
  4. Bzik D. J., Fox B. A., DeLuca N. A., Person S. Nucleotide sequence specifying the glycoprotein gene, gB, of herpes simplex virus type 1. Virology. 1984 Mar;133(2):301–314. doi: 10.1016/0042-6822(84)90397-0. [DOI] [PubMed] [Google Scholar]
  5. Costa R. H., Draper K. G., Kelly T. J., Wagner E. K. An unusual spliced herpes simplex virus type 1 transcript with sequence homology to Epstein-Barr virus DNA. J Virol. 1985 May;54(2):317–328. doi: 10.1128/jvi.54.2.317-328.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cunningham C., Davison A. J. A cosmid-based system for constructing mutants of herpes simplex virus type 1. Virology. 1993 Nov;197(1):116–124. doi: 10.1006/viro.1993.1572. [DOI] [PubMed] [Google Scholar]
  7. Desai P., DeLuca N. A., Glorioso J. C., Person S. Mutations in herpes simplex virus type 1 genes encoding VP5 and VP23 abrogate capsid formation and cleavage of replicated DNA. J Virol. 1993 Mar;67(3):1357–1364. doi: 10.1128/jvi.67.3.1357-1364.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayashida T., Sekiguchi T., Noguchi E., Sunamoto H., Ohba T., Nishimoto T. The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster. Gene. 1994 Apr 20;141(2):267–270. doi: 10.1016/0378-1119(94)90583-5. [DOI] [PubMed] [Google Scholar]
  9. Hisatake K., Hasegawa S., Takada R., Nakatani Y., Horikoshi M., Roeder R. G. The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1. Nature. 1993 Mar 11;362(6416):179–181. doi: 10.1038/362179a0. [DOI] [PubMed] [Google Scholar]
  10. Holland L. E., Sandri-Goldin R. M., Goldin A. L., Glorioso J. C., Levine M. Transcriptional and genetic analyses of the herpes simplex virus type 1 genome: coordinates 0.29 to 0.45. J Virol. 1984 Mar;49(3):947–959. doi: 10.1128/jvi.49.3.947-959.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Locker H., Frenkel N. BamI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. J Virol. 1979 Nov;32(2):429–441. doi: 10.1128/jvi.32.2.429-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Longnecker R., Chatterjee S., Whitley R. J., Roizman B. Identification of a herpes simplex virus 1 glycoprotein gene within a gene cluster dispensable for growth in cell culture. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4303–4307. doi: 10.1073/pnas.84.12.4303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C. W., Linn S., Reinberg D. A human RNA polymerase II complex associated with SRB and DNA-repair proteins. Nature. 1996 May 2;381(6577):86–89. doi: 10.1038/381086a0. [DOI] [PubMed] [Google Scholar]
  14. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J Gen Virol. 1988 Jul;69(Pt 7):1531–1574. doi: 10.1099/0022-1317-69-7-1531. [DOI] [PubMed] [Google Scholar]
  15. McGeoch D. J., Dolan A., Donald S., Brauer D. H. Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1. Nucleic Acids Res. 1986 Feb 25;14(4):1727–1745. doi: 10.1093/nar/14.4.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McGeoch D. J., Dolan A., Donald S., Rixon F. J. Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. J Mol Biol. 1985 Jan 5;181(1):1–13. doi: 10.1016/0022-2836(85)90320-1. [DOI] [PubMed] [Google Scholar]
  17. Mellon I., Rajpal D. K., Koi M., Boland C. R., Champe G. N. Transcription-coupled repair deficiency and mutations in human mismatch repair genes. Science. 1996 Apr 26;272(5261):557–560. doi: 10.1126/science.272.5261.557. [DOI] [PubMed] [Google Scholar]
  18. Mocarski E. S., Roizman B. Structure and role of the herpes simplex virus DNA termini in inversion, circularization and generation of virion DNA. Cell. 1982 Nov;31(1):89–97. doi: 10.1016/0092-8674(82)90408-1. [DOI] [PubMed] [Google Scholar]
  19. Newcomb W. W., Homa F. L., Thomsen D. R., Ye Z., Brown J. C. Cell-free assembly of the herpes simplex virus capsid. J Virol. 1994 Sep;68(9):6059–6063. doi: 10.1128/jvi.68.9.6059-6063.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Patel A. H., Rixon F. J., Cunningham C., Davison A. J. Isolation and characterization of herpes simplex virus type 1 mutants defective in the UL6 gene. Virology. 1996 Mar 1;217(1):111–123. doi: 10.1006/viro.1996.0098. [DOI] [PubMed] [Google Scholar]
  21. Quinn J. P., McGeoch D. J. DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein. Nucleic Acids Res. 1985 Nov 25;13(22):8143–8163. doi: 10.1093/nar/13.22.8143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roizman B. The structure and isomerization of herpes simplex virus genomes. Cell. 1979 Mar;16(3):481–494. doi: 10.1016/0092-8674(79)90023-0. [DOI] [PubMed] [Google Scholar]
  23. Ruppert S., Wang E. H., Tjian R. Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature. 1993 Mar 11;362(6416):175–179. doi: 10.1038/362175a0. [DOI] [PubMed] [Google Scholar]
  24. Sarisky R. T., Weber P. C. Requirement for double-strand breaks but not for specific DNA sequences in herpes simplex virus type 1 genome isomerization events. J Virol. 1994 Jan;68(1):34–47. doi: 10.1128/jvi.68.1.34-47.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sekiguchi T., Nohiro Y., Nakamura Y., Hisamoto N., Nishimoto T. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol. 1991 Jun;11(6):3317–3325. doi: 10.1128/mcb.11.6.3317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sekiguchi T., Yoshida M. C., Sekiguchi M., Nishimoto T. Isolation of a human X chromosome-linked gene essential for progression from G1 to S phase of the cell cycle. Exp Cell Res. 1987 Apr;169(2):395–407. doi: 10.1016/0014-4827(87)90200-x. [DOI] [PubMed] [Google Scholar]
  27. Severini A., Morgan A. R., Tovell D. R., Tyrrell D. L. Study of the structure of replicative intermediates of HSV-1 DNA by pulsed-field gel electrophoresis. Virology. 1994 May 1;200(2):428–435. doi: 10.1006/viro.1994.1206. [DOI] [PubMed] [Google Scholar]
  28. Skare J., Summers W. C. Structure and function of herpesvirus genomes. II. EcoRl, Sbal, and HindIII endonuclease cleavage sites on herpes simplex virus. Virology. 1977 Feb;76(2):581–595. doi: 10.1016/0042-6822(77)90240-9. [DOI] [PubMed] [Google Scholar]
  29. Smiley J. R., Duncan J., Howes M. Sequence requirements for DNA rearrangements induced by the terminal repeat of herpes simplex virus type 1 KOS DNA. J Virol. 1990 Oct;64(10):5036–5050. doi: 10.1128/jvi.64.10.5036-5050.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Szostak J. W., Orr-Weaver T. L., Rothstein R. J., Stahl F. W. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. doi: 10.1016/0092-8674(83)90331-8. [DOI] [PubMed] [Google Scholar]
  31. Tengelsen L. A., Pederson N. E., Shaver P. R., Wathen M. W., Homa F. L. Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants. J Virol. 1993 Jun;67(6):3470–3480. doi: 10.1128/jvi.67.6.3470-3480.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Umene K., Enquist L. W. A deletion analysis of hybrid phage carrying the US region of Herpes simplex virus type 1 (Patton). I. Isolation of deletion derivatives and identification of chi-likes sequences. Gene. 1981 Apr;13(3):251–268. doi: 10.1016/0378-1119(81)90030-5. [DOI] [PubMed] [Google Scholar]
  33. Umene K. Excision of DNA fragments corresponding to the unit-length a sequence of herpes simplex virus type 1 and terminus variation predominate on one side of the excised fragment. J Virol. 1994 Jul;68(7):4377–4383. doi: 10.1128/jvi.68.7.4377-4383.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Umene K. Herpes simplex virus type 1 variant a sequence generated by recombination and breakage of the a sequence in defined regions, including the one involved in recombination. J Virol. 1993 Sep;67(9):5685–5691. doi: 10.1128/jvi.67.9.5685-5691.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Umene K. Intermolecular recombination of the herpes simplex virus type 1 genome analysed using two strains differing in restriction enzyme cleavage sites. J Gen Virol. 1985 Dec;66(Pt 12):2659–2670. doi: 10.1099/0022-1317-66-12-2659. [DOI] [PubMed] [Google Scholar]
  36. Umene K. Recombination of the internal direct repeat element DR2 responsible for the fluidity of the a sequence of herpes simplex virus type 1. J Virol. 1991 Oct;65(10):5410–5416. doi: 10.1128/jvi.65.10.5410-5416.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Umene K. Variability of the region of the herpes simplex virus type 1 genome yielding defective DNA: SmaI fragment polymorphism. Intervirology. 1985;23(3):131–139. doi: 10.1159/000149596. [DOI] [PubMed] [Google Scholar]
  38. Umene K., Yoshida M. Genomic characterization of two predominant genotypes of herpes simplex virus type 1. Arch Virol. 1993;131(1-2):29–46. doi: 10.1007/BF01379078. [DOI] [PubMed] [Google Scholar]
  39. Wang E. H., Tjian R. Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250. Science. 1994 Feb 11;263(5148):811–814. doi: 10.1126/science.8303298. [DOI] [PubMed] [Google Scholar]
  40. Watson R. J., Colberg-Poley A. M., Marcus-Sekura C. J., Carter B. J., Enquist L. W. Characterization of the herpes simplex virus type 1 glycoprotein D mRNA and expression of this protein in Xenopus oocytes. Nucleic Acids Res. 1983 Mar 11;11(5):1507–1522. doi: 10.1093/nar/11.5.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Wilson M. R., Coussens P. M. Purification and characterization of infectious Marek's disease virus genomes using pulsed field electrophoresis. Virology. 1991 Dec;185(2):673–680. doi: 10.1016/0042-6822(91)90538-m. [DOI] [PubMed] [Google Scholar]
  42. Zhang X., Efstathiou S., Simmons A. Identification of novel herpes simplex virus replicative intermediates by field inversion gel electrophoresis: implications for viral DNA amplification strategies. Virology. 1994 Aug 1;202(2):530–539. doi: 10.1006/viro.1994.1375. [DOI] [PubMed] [Google Scholar]
  43. al-Kobaisi M. F., Rixon F. J., McDougall I., Preston V. G. The herpes simplex virus UL33 gene product is required for the assembly of full capsids. Virology. 1991 Jan;180(1):380–388. doi: 10.1016/0042-6822(91)90043-b. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES