Abstract
Foamy viruses, or spumaviruses, are distinct members of the Retroviridae. Here we have characterized the long terminal repeat of the feline, or cat, foamy virus by determining the locations of the transcriptional start site and the poly(A) addition site. The splice donor and splice acceptor sites of the subgenomic mRNA responsible for Pro-Pol protein expression were identified by nucleotide sequencing of the corresponding cDNAs. The leader exon of the feline foamy virus is 57 nucleotides long. The splice acceptor of the subgenomic pol mRNA was found to be located in gag. The location of the splice acceptor of the human foamy virus pol mRNA was confirmed to map in gag. The pol splice acceptor site in gag of the cat foamy virus is located further downstream than that of human foamy virus.
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Selected References
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- Enssle J., Jordan I., Mauer B., Rethwilm A. Foamy virus reverse transcriptase is expressed independently from the Gag protein. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4137–4141. doi: 10.1073/pnas.93.9.4137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flower R. L., Wilcox G. E., Cook R. D., Ellis T. M. Detection and prevalence of serotypes of feline syncytial spumaviruses. Arch Virol. 1985;83(1-2):53–63. doi: 10.1007/BF01310964. [DOI] [PubMed] [Google Scholar]
- Löchelt M., Flügel R. M. The human foamy virus pol gene is expressed as a Pro-Pol polyprotein and not as a Gag-Pol fusion protein. J Virol. 1996 Feb;70(2):1033–1040. doi: 10.1128/jvi.70.2.1033-1040.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Löchelt M., Muranyi W., Flügel R. M. Human foamy virus genome possesses an internal, Bel-1-dependent and functional promoter. Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7317–7321. doi: 10.1073/pnas.90.15.7317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Löchelt M., Zentgraf H., Flügel R. M. Construction of an infectious DNA clone of the full-length human spumaretrovirus genome and mutagenesis of the bel 1 gene. Virology. 1991 Sep;184(1):43–54. doi: 10.1016/0042-6822(91)90820-2. [DOI] [PubMed] [Google Scholar]
- Mergia A., Shaw K. E., Pratt-Lowe E., Barry P. A., Luciw P. A. Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator. J Virol. 1990 Aug;64(8):3598–3604. doi: 10.1128/jvi.64.8.3598-3604.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nosaka T., Ariumi Y., Sakurai M., Takeuchi R., Hatanaka M. Novel internal promoter/enhancer of HTLV-I for Tax expression. Nucleic Acids Res. 1993 Nov 11;21(22):5124–5129. doi: 10.1093/nar/21.22.5124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Renne R., Friedl E., Schweizer M., Fleps U., Turek R., Neumann-Haefelin D. Genomic organization and expression of simian foamy virus type 3 (SFV-3). Virology. 1992 Feb;186(2):597–608. doi: 10.1016/0042-6822(92)90026-l. [DOI] [PubMed] [Google Scholar]
- Yu S. F., Baldwin D. N., Gwynn S. R., Yendapalli S., Linial M. L. Human foamy virus replication: a pathway distinct from that of retroviruses and hepadnaviruses. Science. 1996 Mar 15;271(5255):1579–1582. doi: 10.1126/science.271.5255.1579. [DOI] [PubMed] [Google Scholar]