Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1994 Aug;112(4):1167–1173. doi: 10.1111/j.1476-5381.1994.tb13206.x

Heterogeneity of alpha 1-adrenoceptor subtypes involved in adrenergic contractions of dog blood vessels.

Y Kohno 1, H Saito 1, M Takita 1, S Kigoshi 1, I Muramatsu 1
PMCID: PMC1910263  PMID: 7952878

Abstract

1. We determined the alpha 1-adrenoceptor subtypes involved in adrenergic contractions of eight different blood vessels isolated from the dog. 2. Noradrenaline produced concentration-dependent contractions in all the blood vessels tested, which were competitively inhibited by prazosin, WB4101, HV723 and 5-methylurapidil. However, there was considerable difference between the vessels with regard to the pKB values for all the antagonists. The alpha 1-adrenoceptors of dog vertebral and carotid arteries had high affinity for prazosin (pKB > 9.0) but low affinity for WB4101 (< 8.5), 5-methylurapidil (< 7.5) and HV723 (< or = 8.5). By contrast, HV723 had higher affinity (> 9.0) than prazosin (< 8.3), WB4101 (< 8.7) and 5-methylurapidil (< 8.2) in the portal vein, mesenteric artery and vein, and renal artery. In the femoral artery and vein, however, the four antagonists showed pKB values in the range 8.0-8.7. 3. Chloroethylclonidine (10 microM) produced a remarkable reduction of the contractile responses to noradrenaline in the vertebral and carotid arteries as compared with those in the other vessels. Nifedipine inhibited the responses to noradrenaline in all the tissues tested, and had marked effects in the portal vein. 4. Sympathetic adrenergic contractions induced by transmural electrical stimulation were also inhibited by prazosin and HV723 at different potencies among tissues. The relative potencies of both the antagonists paralleled the relationship in inhibiting the responses to exogenous noradrenaline in each vessel.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARUNLAKSHANA O., SCHILD H. O. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother. 1959 Mar;14(1):48–58. doi: 10.1111/j.1476-5381.1959.tb00928.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bevan J. A., Oriowo M. A., Bevan R. D. Physiological variation in alpha-adrenoceptor-mediated arterial sensitivity: relation to agonist affinity. Science. 1986 Oct 10;234(4773):196–197. doi: 10.1126/science.3018932. [DOI] [PubMed] [Google Scholar]
  3. Bevan J. A., Su C. Sympathetic mechanisms in blood vessels: nerve and muscle relationships. Annu Rev Pharmacol. 1973;13:269–285. doi: 10.1146/annurev.pa.13.040173.001413. [DOI] [PubMed] [Google Scholar]
  4. Burnstock G. Sympathetic purinergic transmission in small blood vessels. Trends Pharmacol Sci. 1988 Apr;9(4):116–117. doi: 10.1016/0165-6147(88)90185-x. [DOI] [PubMed] [Google Scholar]
  5. Cotecchia S., Schwinn D. A., Randall R. R., Lefkowitz R. J., Caron M. G., Kobilka B. K. Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7159–7163. doi: 10.1073/pnas.85.19.7159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Docherty J. R. The pharmacology of alpha 1- and alpha 2-adrenoceptors: evidence for and against a further subdivision. Pharmacol Ther. 1989;44(2):241–284. doi: 10.1016/0163-7258(89)90067-3. [DOI] [PubMed] [Google Scholar]
  7. Drew G. M. What do antagonists tell us about alpha-adrenoceptors? Clin Sci (Lond) 1985;68 (Suppl 10):15s–19s. doi: 10.1042/cs068s015. [DOI] [PubMed] [Google Scholar]
  8. Han C., Abel P. W., Minneman K. P. Alpha 1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle. Nature. 1987 Sep 24;329(6137):333–335. doi: 10.1038/329333a0. [DOI] [PubMed] [Google Scholar]
  9. Ichikawa T., Ishikawa N., Shigei T. Innervation of the canine inferior vena cava. Distribution of adrenergic and cholinergic excitatory fibers among the embryologically distinct segments. Blood Vessels. 1979;16(3):155–162. [PubMed] [Google Scholar]
  10. Lomasney J. W., Cotecchia S., Lefkowitz R. J., Caron M. G. Molecular biology of alpha-adrenergic receptors: implications for receptor classification and for structure-function relationships. Biochim Biophys Acta. 1991 Oct 26;1095(2):127–139. doi: 10.1016/0167-4889(91)90075-9. [DOI] [PubMed] [Google Scholar]
  11. Lundberg J. M., Anggård A., Theodorsson-Norheim E., Pernow J. Guanethidine-sensitive release of neuropeptide Y-like immunoreactivity in the cat spleen by sympathetic nerve stimulation. Neurosci Lett. 1984 Nov 23;52(1-2):175–180. doi: 10.1016/0304-3940(84)90370-7. [DOI] [PubMed] [Google Scholar]
  12. Machaly M., Dalziel H. H., Sneddon P. Evidence for ATP as a cotransmitter in dog mesenteric artery. Eur J Pharmacol. 1988 Feb 16;147(1):83–91. doi: 10.1016/0014-2999(88)90636-x. [DOI] [PubMed] [Google Scholar]
  13. McGrath J. C. Evidence for more than one type of post-junctional alpha-adrenoceptor. Biochem Pharmacol. 1982 Feb 15;31(4):467–484. doi: 10.1016/0006-2952(82)90147-2. [DOI] [PubMed] [Google Scholar]
  14. Minneman K. P. Alpha 1-adrenergic receptor subtypes, inositol phosphates, and sources of cell Ca2+. Pharmacol Rev. 1988 Jun;40(2):87–119. [PubMed] [Google Scholar]
  15. Morrow A. L., Creese I. Characterization of alpha 1-adrenergic receptor subtypes in rat brain: a reevaluation of [3H]WB4104 and [3H]prazosin binding. Mol Pharmacol. 1986 Apr;29(4):321–330. [PubMed] [Google Scholar]
  16. Muramatsu I., Kigoshi S., Ohmura T. Subtypes of alpha 1-adrenoceptors involved in noradrenaline-induced contractions of rat thoracic aorta and dog carotid artery. Jpn J Pharmacol. 1991 Dec;57(4):535–544. doi: 10.1254/jjp.57.535. [DOI] [PubMed] [Google Scholar]
  17. Muramatsu I., Kigoshi S., Oshita M. Nonadrenergic nature of prazosin-resistant, sympathetic contraction in the dog mesenteric artery. J Pharmacol Exp Ther. 1984 May;229(2):532–538. [PubMed] [Google Scholar]
  18. Muramatsu I., Ohmura T., Kigoshi S., Hashimoto S., Oshita M. Pharmacological subclassification of alpha 1-adrenoceptors in vascular smooth muscle. Br J Pharmacol. 1990 Jan;99(1):197–201. doi: 10.1111/j.1476-5381.1990.tb14678.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Muramatsu I., Ohmura T., Oshita M. Comparison between sympathetic adrenergic and purinergic transmission in the dog mesenteric artery. J Physiol. 1989 Apr;411:227–243. doi: 10.1113/jphysiol.1989.sp017570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Muramatsu I. Relation between adrenergic neurogenic contraction and alpha 1-adrenoceptor subtypes in dog mesenteric and carotid arteries and rabbit carotid arteries. Br J Pharmacol. 1991 Jan;102(1):210–214. doi: 10.1111/j.1476-5381.1991.tb12155.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ohmura T., Oshita M., Kigoshi S., Muramatsu I. Identification of alpha 1-adrenoceptor subtypes in the rat vas deferens: binding and functional studies. Br J Pharmacol. 1992 Nov;107(3):697–704. doi: 10.1111/j.1476-5381.1992.tb14509.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Oriowo M. A., Bevan R. D., Bevan J. A. Vascular alpha-adrenoceptor affinity variation is not due to varying populations of subtypes distinguished by WB 4101 and chlorethylclonidine. Eur J Pharmacol. 1992 Jun 17;216(3):415–420. doi: 10.1016/0014-2999(92)90439-b. [DOI] [PubMed] [Google Scholar]
  23. Oriowo M. A., Ruffolo R. R., Jr Heterogeneity of postjunctional alpha 1-adrenoceptors in mammalian aortae: subclassification based on chlorethylclonidine, WB 4101 and nifedipine. J Vasc Res. 1992 Jan-Feb;29(1):33–40. doi: 10.1159/000158929. [DOI] [PubMed] [Google Scholar]
  24. Oshita M., Kigoshi S., Muramatsu I. Pharmacological characterization of two distinct alpha 1-adrenoceptor subtypes in rabbit thoracic aorta. Br J Pharmacol. 1993 Apr;108(4):1071–1076. doi: 10.1111/j.1476-5381.1993.tb13507.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Oshita M., Kigoshi S., Muramatsu I. Three distinct binding sites for [3H]-prazosin in the rat cerebral cortex. Br J Pharmacol. 1991 Dec;104(4):961–965. doi: 10.1111/j.1476-5381.1991.tb12533.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Perez D. M., Piascik M. T., Graham R. M. Solution-phase library screening for the identification of rare clones: isolation of an alpha 1D-adrenergic receptor cDNA. Mol Pharmacol. 1991 Dec;40(6):876–883. [PubMed] [Google Scholar]
  27. Sayet I., Neuilly G., Rakotoarisoa L., Mironneau J., Mironneau C. Rat vena cava alpha 1B-adrenoceptors: characterization by [3H]prazosin binding and contraction experiments. Eur J Pharmacol. 1993 Aug 15;246(3):275–281. doi: 10.1016/0922-4106(93)90042-8. [DOI] [PubMed] [Google Scholar]
  28. Schwinn D. A., Lomasney J. W., Lorenz W., Szklut P. J., Fremeau R. T., Jr, Yang-Feng T. L., Caron M. G., Lefkowitz R. J., Cotecchia S. Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem. 1990 May 15;265(14):8183–8189. [PubMed] [Google Scholar]
  29. Schwinn D. A., Lomasney J. W. Pharmacologic characterization of cloned alpha 1-adrenoceptor subtypes: selective antagonists suggest the existence of a fourth subtype. Eur J Pharmacol. 1992 Dec 1;227(4):433–436. doi: 10.1016/0922-4106(92)90162-o. [DOI] [PubMed] [Google Scholar]
  30. Takayanagi I., Koike K., Kawano K., Onozuka S. A regional difference in alpha 1-adrenoceptors in canine arteries. Arch Int Pharmacodyn Ther. 1988 Jul-Aug;294:175–184. [PubMed] [Google Scholar]
  31. Voigt M. M., Kispert J., Chin H. M. Sequence of a rat brain cDNA encoding an alpha-1B adrenergic receptor. Nucleic Acids Res. 1990 Feb 25;18(4):1053–1053. doi: 10.1093/nar/18.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES