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. 1994 May;112(1):299–303. doi: 10.1111/j.1476-5381.1994.tb13068.x

Dual effects of mastoparan on intracellular free Ca2+ concentrations in human astrocytoma cells.

N Nakahata 1, H Ishimoto 1, K Mizuno 1, Y Ohizumi 1, H Nakanishi 1
PMCID: PMC1910271  PMID: 8032654

Abstract

1. The effect of mastoparan, a wasp venom toxin, on intracellular free Ca2+ concentration ([Ca2+]i) was examined in human astrocytoma cells. Mastoparan inhibited [Ca2+]i induced by carbachol (100 microM) in a concentration-dependent manner in the absence of extracellular Ca2+, consistent with our previous results showing that mastoparan inhibits phosphoinositide hydrolysis in human astrocytoma cells. 2. In contrast, mastoparan itself increased [Ca2+]i and augmented carbachol-induced increase in the [Ca2+]i in the presence of extracellular Ca2+, suggesting that mastoparan elicited Ca2+ influx from the extracellular medium. The increase appeared to be maximum at extracellular Ca2+ concentrations of 0.1-0.2 mM. The higher concentrations of extracellular Ca2+ depressed the influx. 3. Pertussis toxin did not affect mastoparan-induced inhibition of [Ca2+]i in the absence of extracellular Ca2+, consistent with the previous results that pertussis toxin did not affect mastoparan-induced inhibition of phosphoinositide hydrolysis. 4. Pertussis toxin augmented mastoparan-induced increase in [Ca2+]i in the presence of extracellular Ca2+, suggesting that pertussis toxin substrate(s) seems to be inhibitory for Ca2+ influx induced by mastoparan. 5. Verapamil, nifedipine and diltiazem (each 10 microM), L-type Ca2+ antagonists, did not affect mastoparan-induced Ca2+ influx. However, verapamil (10 microM) slightly inhibited the increase in [Ca2+]i induced by carbachol in the presence of mastoparan. 6. The results obtained in the present study indicate that mastoparan has two opposite effects on [Ca2+]i in human astrocytoma cells and possibly has at least two sites of action.

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Selected References

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  1. Denker B. M., Tempst P., Neer E. J. Characterization of a mastoparan-stimulated nucleotidase from bovine brain. Biochem J. 1991 Sep 1;278(Pt 2):341–345. doi: 10.1042/bj2780341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gil J., Higgins T., Rozengurt E. Mastoparan, a novel mitogen for Swiss 3T3 cells, stimulates pertussis toxin-sensitive arachidonic acid release without inositol phosphate accumulation. J Cell Biol. 1991 May;113(4):943–950. doi: 10.1083/jcb.113.4.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gusovsky F., Soergel D. G., Daly J. W. Effects of mastoparan and related peptides on phosphoinositide breakdown in HL-60 cells and cell-free preparations. Eur J Pharmacol. 1991 Apr 25;206(4):309–314. doi: 10.1016/0922-4106(91)90115-x. [DOI] [PubMed] [Google Scholar]
  4. Hepler J. R., Hughes A. R., Harden T. K. Evidence that muscarinic cholinergic receptors selectively interact with either the cyclic AMP or the inositol phosphate second-messenger response systems. Biochem J. 1987 Nov 1;247(3):793–796. doi: 10.1042/bj2470793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Higashijima T., Burnier J., Ross E. M. Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity. J Biol Chem. 1990 Aug 25;265(24):14176–14186. [PubMed] [Google Scholar]
  6. Higashijima T., Ross E. M. Mapping of the mastoparan-binding site on G proteins. Cross-linking of [125I-Tyr3,Cys11]mastoparan to Go. J Biol Chem. 1991 Jul 5;266(19):12655–12661. [PubMed] [Google Scholar]
  7. Higashijima T., Uzu S., Nakajima T., Ross E. M. Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins (G proteins). J Biol Chem. 1988 May 15;263(14):6491–6494. [PubMed] [Google Scholar]
  8. Higashijima T., Wakamatsu K., Takemitsu M., Fujino M., Nakajima T., Miyazawa T. Conformational change of mastoparan from wasp venom on binding with phospholipid membrane. FEBS Lett. 1983 Feb 21;152(2):227–230. doi: 10.1016/0014-5793(83)80385-8. [DOI] [PubMed] [Google Scholar]
  9. Hirai Y., Yasuhara T., Yoshida H., Nakajima T., Fujino M., Kitada C. A new mast cell degranulating peptide "mastoparan" in the venom of Vespula lewisii. Chem Pharm Bull (Tokyo) 1979 Aug;27(8):1942–1944. doi: 10.1248/cpb.27.1942. [DOI] [PubMed] [Google Scholar]
  10. Kikkawa S., Takahashi K., Takahashi K., Shimada N., Ui M., Kimura N., Katada T. Activation of nucleoside diphosphate kinase by mastoparan, a peptide isolated from wasp venom. FEBS Lett. 1992 Jul 6;305(3):237–240. doi: 10.1016/0014-5793(92)80676-8. [DOI] [PubMed] [Google Scholar]
  11. Koch G., Mohr C., Just I., Aktories K. Posttranslational isoprenylation of rho protein is a prerequisite for its interaction with mastoparan and other amphiphilic agents. Biochem Biophys Res Commun. 1992 Jul 15;186(1):448–454. doi: 10.1016/s0006-291x(05)80828-9. [DOI] [PubMed] [Google Scholar]
  12. Masters S. B., Martin M. W., Harden T. K., Brown J. H. Pertussis toxin does not inhibit muscarinic-receptor-mediated phosphoinositide hydrolysis or calcium mobilization. Biochem J. 1985 May 1;227(3):933–937. doi: 10.1042/bj2270933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakahata N., Abe M. T., Matsuoka I., Nakanishi H. Mastoparan inhibits phosphoinositide hydrolysis via pertussis toxin-insensitive [corrected] G-protein in human astrocytoma cells. FEBS Lett. 1990 Jan 15;260(1):91–94. doi: 10.1016/0014-5793(90)80074-s. [DOI] [PubMed] [Google Scholar]
  14. Nakahata N., Martin M. W., Hughes A. R., Hepler J. R., Harden T. K. H1-histamine receptors on human astrocytoma cells. Mol Pharmacol. 1986 Feb;29(2):188–195. [PubMed] [Google Scholar]
  15. Nakahata N., Matsuoka I., Ono T., Nakanishi H. Thromboxane A2 activates phospholipase C in astrocytoma cells via pertussis toxin-insensitive G-protein. Eur J Pharmacol. 1989 Mar 29;162(3):407–417. doi: 10.1016/0014-2999(89)90331-2. [DOI] [PubMed] [Google Scholar]
  16. Norgauer J., Eberle M., Lemke H. D., Aktories K. Activation of human neutrophils by mastoparan. Reorganization of the cytoskeleton, formation of phosphatidylinositol 3,4,5-trisphosphate, secretion up-regulation of complement receptor type 3 and superoxide anion production are stimulated by mastoparan. Biochem J. 1992 Mar 1;282(Pt 2):393–397. doi: 10.1042/bj2820393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okano Y., Takagi H., Tohmatsu T., Nakashima S., Kuroda Y., Saito K., Nozawa Y. A wasp venom mastoparan-induced polyphosphoinositide breakdown in rat peritoneal mast cells. FEBS Lett. 1985 Sep 2;188(2):363–366. doi: 10.1016/0014-5793(85)80403-8. [DOI] [PubMed] [Google Scholar]
  18. Raynor R. L., Zheng B., Kuo J. F. Membrane interactions of amphiphilic polypeptides mastoparan, melittin, polymyxin B, and cardiotoxin. Differential inhibition of protein kinase C, Ca2+/calmodulin-dependent protein kinase II and synaptosomal membrane Na,K-ATPase, and Na+ pump and differentiation of HL60 cells. J Biol Chem. 1991 Feb 15;266(5):2753–2758. [PubMed] [Google Scholar]
  19. Tanimura A., Matsumoto Y., Tojyo Y. Mastoparan increases membrane permeability in rat parotid cells independently of action on G-proteins. Biochem Biophys Res Commun. 1991 Jun 14;177(2):802–808. doi: 10.1016/0006-291x(91)91860-f. [DOI] [PubMed] [Google Scholar]
  20. Tomita U., Inanobe A., Kobayashi I., Takahashi K., Ui M., Katada T. Direct interactions of mastoparan and compound 48/80 with GTP-binding proteins. J Biochem. 1991 Jan;109(1):184–189. doi: 10.1093/oxfordjournals.jbchem.a123342. [DOI] [PubMed] [Google Scholar]
  21. Wojcikiewicz R. J., Nahorski S. R. Phosphoinositide hydrolysis in permeabilized SH-SY5Y human neuroblastoma cells is inhibited by mastoparan. FEBS Lett. 1989 Apr 24;247(2):341–344. doi: 10.1016/0014-5793(89)81366-3. [DOI] [PubMed] [Google Scholar]
  22. Yanai K., Maeyama K., Nakahata N., Nakanishi H., Watanabe T. Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): comparison of histamine-induced calcium mobilization. Naunyn Schmiedebergs Arch Pharmacol. 1992 Jul;346(1):51–56. doi: 10.1007/BF00167570. [DOI] [PubMed] [Google Scholar]
  23. Yule D. I., Williams J. A. Mastoparan induces oscillations of cytosolic Ca2+ in rat pancreatic acinar cells. Biochem Biophys Res Commun. 1991 May 31;177(1):159–165. doi: 10.1016/0006-291x(91)91962-c. [DOI] [PubMed] [Google Scholar]

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