Skip to main content
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1994 May;112(1):93–96. doi: 10.1111/j.1476-5381.1994.tb13035.x

Comparative analysis of the vagal stimulation of gastric acid secretion in rodent isolated stomach preparations.

N J Welsh 1, N P Shankley 1, J W Black 1
PMCID: PMC1910289  PMID: 7913382

Abstract

1. Electrical field stimulation produced a tetrodotoxin-sensitive, frequency-dependent, release of acid from isolated, lumen-perfused, stomach preparations from mouse, immature rat and guinea-pig. 2. In the guinea-pig and mouse preparations, the frequency-dependent response was abolished by hexamethonium, acetylcholine (ACh) muscarinic (M) and histamine H2-receptor blockade, consistent with the hypothesis that the vagal ACh acts indirectly by stimulating the release of endogenous histamine. 3. In contrast, in the rat preparation the frequency-dependent response was partially refractory to all of these inhibitors. However, a combination of H2- and ACh M-receptor blockade did abolish the effect. 4. We conclude that vagal-stimulated acid secretion in the rat, unlike the other two species, behaves as though there is a direct innervation of the oxyntic cells by either cholinergic or noncholinergic neurones.

Full text

PDF
93

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angus J. A., Black J. W. The interaction of choline esters, vagal stimulation and H2-receptor blockade on acid secretion in vitro. Eur J Pharmacol. 1982 May 21;80(2-3):217–224. doi: 10.1016/0014-2999(82)90057-7. [DOI] [PubMed] [Google Scholar]
  2. Baird A. W., Main I. H. Characterisation of acid secretory responses of the rat isolated gastric mucosa to electrical field stimulation [proceedings]. Br J Pharmacol. 1978 Nov;64(3):445P–446P. [PMC free article] [PubMed] [Google Scholar]
  3. Black J. W., Shankley N. P. Pharmacological analysis of muscarinic receptors coupled to oxyntic cell secretion in the mouse stomach. Br J Pharmacol. 1985 Nov;86(3):601–607. doi: 10.1111/j.1476-5381.1985.tb08936.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Black J. W., Shankley N. P. Pharmacological analysis of the inhibition by pirenzepine and atropine of vagal-stimulated acid secretion in the isolated stomach of the mouse. Br J Pharmacol. 1986 May;88(1):291–297. doi: 10.1111/j.1476-5381.1986.tb09498.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Black J. W., Shankley N. P. The isolated stomach preparation of the mouse: a physiological unit for pharmacological analysis. Br J Pharmacol. 1985 Nov;86(3):571–579. doi: 10.1111/j.1476-5381.1985.tb08933.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grossman M. I., Konturek S. J. Inhibition of acid secretion in dog by metiamide, a histamine antagonist acting on H2 receptors. Gastroenterology. 1974 Apr;66(4):517–521. [PubMed] [Google Scholar]
  7. Horwell D. C., Hughes J., Hunter J. C., Pritchard M. C., Richardson R. S., Roberts E., Woodruff G. N. Rationally designed "dipeptoid" analogues of CCK. alpha-Methyltryptophan derivatives as highly selective and orally active gastrin and CCK-B antagonists with potent anxiolytic properties. J Med Chem. 1991 Jan;34(1):404–414. doi: 10.1021/jm00105a062. [DOI] [PubMed] [Google Scholar]
  8. Newson B., Dahlström A., Enerbäck L., Ahlman H. Suggestive evidence for a direct innervation of mucosal mast cells. Neuroscience. 1983 Oct;10(2):565–570. doi: 10.1016/0306-4522(83)90153-7. [DOI] [PubMed] [Google Scholar]
  9. OLBE L. EFFECT OF RESECTION OF GASTRIN RELEASING REGIONS ON ACID RESPONSE TO SHAM FEEDING AND INSULIN HYPOGLYCEMIA IN PAVLOV POUCH DOGS. Acta Physiol Scand. 1964 Sep-Oct;62:169–175. doi: 10.1111/j.1748-1716.1964.tb03965.x. [DOI] [PubMed] [Google Scholar]
  10. Patel M., Spraggs C. F. Functional comparisons of gastrin/cholecystokinin receptors in isolated preparations of gastric mucosa and ileum. Br J Pharmacol. 1992 Jun;106(2):275–282. doi: 10.1111/j.1476-5381.1992.tb14328.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Uvnäs-Wallensten K., Rehfeld J. F., Larsson L. I., Uvnäs B. Heptadecapeptide gastrin in the vagal nerve. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5707–5710. doi: 10.1073/pnas.74.12.5707. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES