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. 1994 May;112(1):23–30. doi: 10.1111/j.1476-5381.1994.tb13023.x

Regulation of aromatic L-amino acid decarboxylase in rat striatal synaptosomes: effects of dopamine receptor agonists and antagonists.

M Y Zhu 1, A V Juorio 1, I A Paterson 1, A A Boulton 1
PMCID: PMC1910301  PMID: 7913379

Abstract

1. In this study we investigated the effects of dopamine receptor agonists and antagonists on rat striatal synaptosomal aromatic L-amino acid decarboxylase (AADC) activity. 2. The results show that 10(-5)-10(-7) M cis-flupenthixol increased the striatal synaptosomal AADC activity (by 25% to 57%) in a time-dependent manner. SCH 23390 and remoxipride alone had little or no effect on striatal synaptosomal AADC activity, but in combination they increased AADC activity by 20%, suggesting that the increases in striatal synaptosomal AADC activity occurred only after blockade of both dopamine D1 and D2 receptors. 3. Treatment with (+)-amphetamine and (+/-)-2-(N-phenylethyl-N-propyl)amino-5- hydroxytetralin hydrochloride ((+/-)-PPHT) produced a reduction of striatal synaptosomal AADC activity in a concentration- and time-dependent manner. SKF 38393 and (-)-quinpirole, however, exhibited no effect on striatal synaptosomal AADC activity, suggesting that only the mixed dopamine receptor agonists can reduce the AADC activity. Incubation with apomorphine at a concentration of 10(-4) M inhibited the AADC activity by 74% and this inhibition cannot be antagonized by SCH 23390, remoxipride or cis-flupenthixol, suggesting that apomorphine-induced inhibition of striatal synaptosomal AADC activity was not mediated by dopamine receptors. 4. cis-Flupenthixol can reverse the reduction of AADC activity induced by (+)-amphetamine and (+/-)-PPHT. The inhibition of AADC activity elicited by (+/-)-PPHT also can be reversed by SCH 23390 and remoxipride. 5. The inhibition of striatal synaptosomal AADC activity induced by (+/-)-PPHT is calcium-dependent and protein kinase C may play a role in the regulation of striatal AADC activity.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bitran M., Bustos G. On the mechanism of presynaptic autoreceptor-mediated inhibition of transmitter synthesis in dopaminergic nerve terminals. Biochem Pharmacol. 1982 Sep 15;31(18):2851–2860. doi: 10.1016/0006-2952(82)90254-4. [DOI] [PubMed] [Google Scholar]
  2. Bräutigam M., Dreesen R., Herken H. Dopa-release from mouse neuroblastoma clone N 1 E-115 into the culture medium. A test for tyrosine hydroxylase activity. Naunyn Schmiedebergs Arch Pharmacol. 1982 Aug;320(2):85–89. doi: 10.1007/BF00506305. [DOI] [PubMed] [Google Scholar]
  3. Buckland P. R., O'Donovan M. C., McGuffin P. Changes in dopa decarboxylase mRNA but not tyrosine hydroxylase mRNA levels in rat brain following antipsychotic treatment. Psychopharmacology (Berl) 1992;108(1-2):98–102. doi: 10.1007/BF02245292. [DOI] [PubMed] [Google Scholar]
  4. Buckland P. R., O'Donovan M. C., McGuffin P. Changes in dopamine D1, D2 and D3 receptor mRNA levels in rat brain following antipsychotic treatment. Psychopharmacology (Berl) 1992;106(4):479–483. doi: 10.1007/BF02244818. [DOI] [PubMed] [Google Scholar]
  5. Carlson J. H., Bergstrom D. A., Walters J. R. Stimulation of both D1 and D2 dopamine receptors appears necessary for full expression of postsynaptic effects of dopamine agonists: a neurophysiological study. Brain Res. 1987 Jan 6;400(2):205–218. doi: 10.1016/0006-8993(87)90619-6. [DOI] [PubMed] [Google Scholar]
  6. Castagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., Nishizuka Y. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982 Jul 10;257(13):7847–7851. [PubMed] [Google Scholar]
  7. Christiansen J., Squires R. F. Antagonistic effects of apomorphine and haloperidol on rat striatal synaptosomal tyrosine hydroxylase. J Pharm Pharmacol. 1974 May;26(5):367–369. doi: 10.1111/j.2042-7158.1974.tb09293.x. [DOI] [PubMed] [Google Scholar]
  8. Clark D., White F. J. D1 dopamine receptor--the search for a function: a critical evaluation of the D1/D2 dopamine receptor classification and its functional implications. Synapse. 1987;1(4):347–388. doi: 10.1002/syn.890010408. [DOI] [PubMed] [Google Scholar]
  9. Cotman C. W. Isolation of synaptosomal and synaptic plasma membrane fractions. Methods Enzymol. 1974;31:445–452. doi: 10.1016/0076-6879(74)31050-6. [DOI] [PubMed] [Google Scholar]
  10. Deffond D., Durif F., Tournilhac M. Apomorphine in treatment of Parkinson's disease: comparison between subcutaneous and sublingual routes. J Neurol Neurosurg Psychiatry. 1993 Jan;56(1):101–103. doi: 10.1136/jnnp.56.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Delanoy R. L., Dunn A. J. Effects of haloperidol and apomorphine on catecholamine metabolism in brain slices. Reserpine-like effects of haloperidol. Biochem Pharmacol. 1982 Oct 15;31(20):3297–3305. doi: 10.1016/0006-2952(82)90564-0. [DOI] [PubMed] [Google Scholar]
  12. Frankel J. P., Lees A. J., Kempster P. A., Stern G. M. Subcutaneous apomorphine in the treatment of Parkinson's disease. J Neurol Neurosurg Psychiatry. 1990 Feb;53(2):96–101. doi: 10.1136/jnnp.53.2.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gardner C. R., Richards M. H. Use of D,L-alpha-monofluoromethyldopa to distinguish subcellular pools of aromatic amino acid decarboxylase in mouse brain. Brain Res. 1981 Jul 20;216(2):291–298. doi: 10.1016/0006-8993(81)90131-1. [DOI] [PubMed] [Google Scholar]
  14. Gershanik O., Heikkila R. E., Duvoisin R. C. Behavioral correlations of dopamine receptor activation. Neurology. 1983 Nov;33(11):1489–1492. doi: 10.1212/wnl.33.11.1489. [DOI] [PubMed] [Google Scholar]
  15. Goldstein M., Freedman L. S., Backstrom T. The inhibition of catecholamine biosynthesis by apomorphine. J Pharm Pharmacol. 1970 Sep;22(9):715–717. doi: 10.1111/j.2042-7158.1970.tb12763.x. [DOI] [PubMed] [Google Scholar]
  16. Hadjiconstantinou M., Rossetti Z., Silvia C., Krajnc D., Neff N. H. Aromatic L-amino acid decarboxylase activity of the rat retina is modulated in vivo by environmental light. J Neurochem. 1988 Nov;51(5):1560–1564. doi: 10.1111/j.1471-4159.1988.tb01125.x. [DOI] [PubMed] [Google Scholar]
  17. Hadjiconstantinou M., Wemlinger T. A., Sylvia C. P., Hubble J. P., Neff N. H. Aromatic L-amino acid decarboxylase activity of mouse striatum is modulated via dopamine receptors. J Neurochem. 1993 Jun;60(6):2175–2180. doi: 10.1111/j.1471-4159.1993.tb03503.x. [DOI] [PubMed] [Google Scholar]
  18. Hidaka H., Inagaki M., Kawamoto S., Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984 Oct 9;23(21):5036–5041. doi: 10.1021/bi00316a032. [DOI] [PubMed] [Google Scholar]
  19. Joh T. H., Park D. H., Reis D. J. Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4744–4748. doi: 10.1073/pnas.75.10.4744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kehr W., Carlsson A., Lindqvist M., Magnusson T., Atack C. Evidence for a receptor-mediated feedback control of striatal tyrosine hydroxylase activity. J Pharm Pharmacol. 1972 Sep;24(9):744–747. doi: 10.1111/j.2042-7158.1972.tb09104.x. [DOI] [PubMed] [Google Scholar]
  21. Kurata K., Shibata R. Effects of D1 and D2 antagonists on the transient increase of dopamine release by dopamine agonists by means of brain dialysis. Neurosci Lett. 1991 Nov 25;133(1):77–80. doi: 10.1016/0304-3940(91)90061-w. [DOI] [PubMed] [Google Scholar]
  22. LOVENBERG W., WEISSBACH H., UDENFRIEND S. Aromatic L-amino acid decarboxylase. J Biol Chem. 1962 Jan;237:89–93. [PubMed] [Google Scholar]
  23. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  24. Lieberman A., Goldstein M., Neophytides A., Kupersmith M., Leibowitz M., Zasorin N., Walker R., Kleinberg D. Lisuride in Parkinson disease: efficacy of lisuride compared to levodopa. Neurology. 1981 Aug;31(8):961–965. doi: 10.1212/wnl.31.8.961. [DOI] [PubMed] [Google Scholar]
  25. Magoski N. S., Walz W. Ionic dependence of a P2-purinoceptor mediated depolarization of cultured astrocytes. J Neurosci Res. 1992 Aug;32(4):530–538. doi: 10.1002/jnr.490320408. [DOI] [PubMed] [Google Scholar]
  26. Nagatsu T., Yamamoto T., Kato T. A new and highly sensitive voltammetric assay for aromatic L-amino acid decarboxylase activity by high-performance liquid chromatography. Anal Biochem. 1979 Nov 15;100(1):160–165. doi: 10.1016/0003-2697(79)90126-x. [DOI] [PubMed] [Google Scholar]
  27. Nishizuka Y. Studies and perspectives of protein kinase C. Science. 1986 Jul 18;233(4761):305–312. doi: 10.1126/science.3014651. [DOI] [PubMed] [Google Scholar]
  28. Okuno S., Fujisawa H. Accurate assay of dopa decarboxylase by preventing nonenzymatic decarboxylation of dopa. Anal Biochem. 1983 Mar;129(2):412–415. doi: 10.1016/0003-2697(83)90570-5. [DOI] [PubMed] [Google Scholar]
  29. Piomelli D., Pilon C., Giros B., Sokoloff P., Martres M. P., Schwartz J. C. Dopamine activation of the arachidonic acid cascade as a basis for D1/D2 receptor synergism. Nature. 1991 Sep 12;353(6340):164–167. doi: 10.1038/353164a0. [DOI] [PubMed] [Google Scholar]
  30. Raese J. D., Edelman A. M., Makk G., Bruckwick E. A., Lovenberg W., Barchas J. D. Brain striatal tyrosine hydroxylase: activation of the enzyme by cyclic AMP-independent phosphorylation. Commun Psychopharmacol. 1979;3(5):295–301. [PubMed] [Google Scholar]
  31. Rosengarten H., Schweitzer J. W., Friedhoff A. J. Selective dopamine D2 receptor reduction enhances a D1-mediated oral dyskinesia in rats. Life Sci. 1986 Jul 7;39(1):29–35. doi: 10.1016/0024-3205(86)90434-0. [DOI] [PubMed] [Google Scholar]
  32. Rossetti Z. L., Silvia C. P., Krajnc D., Neff N. H., Hadjiconstantinou M. Aromatic L-amino acid decarboxylase is modulated by D1 dopamine receptors in rat retina. J Neurochem. 1990 Mar;54(3):787–791. doi: 10.1111/j.1471-4159.1990.tb02320.x. [DOI] [PubMed] [Google Scholar]
  33. Saavedra J. M. Enzymatic isotopic assay for and presence of beta-phenylethylamine in brain. J Neurochem. 1974 Feb;22(2):211–216. doi: 10.1111/j.1471-4159.1974.tb11581.x. [DOI] [PubMed] [Google Scholar]
  34. Seeman P., Watanabe M., Grigoriadis D., Tedesco J. L., George S. R., Svensson U., Nilsson J. L., Neumeyer J. L. Dopamine D2 receptor binding sites for agonists. A tetrahedral model. Mol Pharmacol. 1985 Nov;28(5):391–399. [PubMed] [Google Scholar]
  35. Seiler M. P., Stoll A. P., Closse A., Frick W., Jaton A., Vigouret J. M. Structure-activity relationships of dopaminergic 5-hydroxy-2-aminotetralin derivatives with functionalized N-alkyl substituents. J Med Chem. 1986 Jun;29(6):912–917. doi: 10.1021/jm00156a007. [DOI] [PubMed] [Google Scholar]
  36. Sims K. L., Davis G. A., Bloom F. E. Activities of 3,4-dihydroxy-L-phenylalanine and 5-hydroxy-L-tryptophan decarboxylases in rat brain: assay characteristics and distribution. J Neurochem. 1973 Feb;20(2):449–464. doi: 10.1111/j.1471-4159.1973.tb12144.x. [DOI] [PubMed] [Google Scholar]
  37. Stoof J. C., Kebabian J. W. Opposing roles for D-1 and D-2 dopamine receptors in efflux of cyclic AMP from rat neostriatum. Nature. 1981 Nov 26;294(5839):366–368. doi: 10.1038/294366a0. [DOI] [PubMed] [Google Scholar]
  38. Stoof J. C., Kebabian J. W. Two dopamine receptors: biochemistry, physiology and pharmacology. Life Sci. 1984 Dec 3;35(23):2281–2296. doi: 10.1016/0024-3205(84)90519-8. [DOI] [PubMed] [Google Scholar]
  39. Vulliet P. R., Woodgett J. R., Cohen P. Phosphorylation of tyrosine hydroxylase by calmodulin-dependent multiprotein kinase. J Biol Chem. 1984 Nov 25;259(22):13680–13683. [PubMed] [Google Scholar]
  40. Walters J. R., Bergstrom D. A., Carlson J. H., Chase T. N., Braun A. R. D1 dopamine receptor activation required for postsynaptic expression of D2 agonist effects. Science. 1987 May 8;236(4802):719–722. doi: 10.1126/science.2953072. [DOI] [PubMed] [Google Scholar]
  41. Walters J. R., Roth R. H. Dopaminergic neurons: an in vivo system for measuring drug interactions with presynaptic receptors. Naunyn Schmiedebergs Arch Pharmacol. 1976 Dec;296(1):5–14. doi: 10.1007/BF00498834. [DOI] [PubMed] [Google Scholar]
  42. Weick B. G., Walters J. R. Effects of D1 and D2 dopamine receptor stimulation on the activity of substantia nigra pars reticulata neurons in 6-hydroxydopamine lesioned rats: D1/D2 coactivation induces potentiated responses. Brain Res. 1987 Mar 10;405(2):234–246. doi: 10.1016/0006-8993(87)90293-9. [DOI] [PubMed] [Google Scholar]
  43. Westerink B. H., Horn A. S. Do neuroleptics prevent the penetration of dopamine agonists into the brain? Eur J Pharmacol. 1979 Sep 1;58(1):39–48. doi: 10.1016/0014-2999(79)90337-6. [DOI] [PubMed] [Google Scholar]
  44. White F. J., Wang R. Y. Electrophysiological evidence for the existence of both D-1 and D-2 dopamine receptors in the rat nucleus accumbens. J Neurosci. 1986 Jan;6(1):274–280. doi: 10.1523/JNEUROSCI.06-01-00274.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Wood J. D., Russell M. P., Kurylo E., Newstead J. D. Stability of synaptosomal GABA levels and their use in determining the in vivo effects of drugs: convulsant agents. J Neurochem. 1979 Jul;33(1):61–68. doi: 10.1111/j.1471-4159.1979.tb11706.x. [DOI] [PubMed] [Google Scholar]
  46. Young E. A., Neff N. H., Hadjiconstantinou M. Evidence for cyclic AMP-mediated increase of aromatic L-amino acid decarboxylase activity in the striatum and midbrain. J Neurochem. 1993 Jun;60(6):2331–2333. doi: 10.1111/j.1471-4159.1993.tb03525.x. [DOI] [PubMed] [Google Scholar]
  47. Zhu M. Y., Juorio A. V., Paterson I. A., Boulton A. A. Regulation of aromatic L-amino acid decarboxylase by dopamine receptors in the rat brain. J Neurochem. 1992 Feb;58(2):636–641. doi: 10.1111/j.1471-4159.1992.tb09765.x. [DOI] [PubMed] [Google Scholar]
  48. Zhu M. Y., Juorio A. V., Paterson I. A., Boulton A. A. Regulation of striatal aromatic L-amino acid decarboxylase: effects of blockade or activation of dopamine receptors. Eur J Pharmacol. 1993 Jul 20;238(2-3):157–164. doi: 10.1016/0014-2999(93)90843-7. [DOI] [PubMed] [Google Scholar]

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