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. 1974 Jan;74(1):125–136.

Radiation-Induced Cardiomyopathy

II. An Electron Microscopic Study of Myocardial Microvasculature

M Yusuf Khan, M Ohanian
PMCID: PMC1910718  PMID: 19971051

Abstract

Sequential ultrastructural lesions of rabbit myocardial microvasculature after a single dose (1008 or 1300 rads) of local x-irradiation are described. Vascular permeability status was assessed through use of ferritin and colloidal carbon. Endothelial cell swelling and increased vascular permeability were the most conspicuous lesions during the first week following irradiation. Increased vascular permeability, as indicated by the observed ferritin and carbon distribution, appears to be a result of altered pinocytotic transport and widening of endothelial junctional gaps. These lesions, on day 14 and later, were followed by basement membrane thickening, endothelial cell extrusions and bleb formation, platelet sequestration, abnormal endothelial cell phagocytosis and appearance of myelin-like figures within the endothelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashford T. P., Frieiman D. G. The role of the endothelium in the initial phases of thrombosis. An electron microscopic study. Am J Pathol. 1967 Feb;50(2):257–273. [PMC free article] [PubMed] [Google Scholar]
  2. BARI W. A., SORENSON G. D. ULTRASTRUCTURAL ALTERATIONS IN X-IRRADIATED SPLEEN. Pathol Microbiol (Basel) 1964;27:257–275. doi: 10.1159/000161473. [DOI] [PubMed] [Google Scholar]
  3. BENACERRAF B., McCLUSKEY R. T., PATRAS D. Localization of colloidal substances in vascular endothelium: a mechanism of tissue damage. I. Factors causing the pathologic deposition of colloidal carbon. Am J Pathol. 1959 Jan-Feb;35(1):75–91. [PMC free article] [PubMed] [Google Scholar]
  4. Bruns R. R., Palade G. E. Studies on blood capillaries. I. General organization of blood capillaries in muscle. J Cell Biol. 1968 May;37(2):244–276. doi: 10.1083/jcb.37.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bruns R. R., Palade G. E. Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries. J Cell Biol. 1968 May;37(2):277–299. doi: 10.1083/jcb.37.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COTRAN R. S. ENDOTHELIAL PHAGOCYTOSIS: AN ELECTRON-MICROSPOPIC STUDY. Exp Mol Pathol. 1965 Apr;28:217–231. doi: 10.1016/0014-4800(65)90034-1. [DOI] [PubMed] [Google Scholar]
  7. Cotran R. S., Suter E. R., Majno G. The use of colloidal carbon as a tracer for vascular injury. A review. Vasc Dis. 1967 Apr;4(2):107–127. [PubMed] [Google Scholar]
  8. Hoff H. F., Gottlob R. A fine structure study of injury to the endothelial cells of the rabbit abdominal aorta by various stimuli. Angiology. 1967 Jul;18(7):440–451. doi: 10.1177/000331976701800704. [DOI] [PubMed] [Google Scholar]
  9. Jorgensen L., Rowsell H. C., Hovig T., Glynn M. F., Mustard J. F. Adenosine diphosphate-induced platelet aggregation and myocardial infarction in swine. Lab Invest. 1967 Dec;17(6):616–644. [PubMed] [Google Scholar]
  10. Keyeux A., Dunjic A., Royer E., Jovanovic D., Van de Merckt J. Late functional and circulatory changes in rats after local irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1971;20(1):7–25. doi: 10.1080/09553007114550831. [DOI] [PubMed] [Google Scholar]
  11. Khan M. Y. Radiation-induced cardiomyopathy. I. An electron microscopic study of cardiac muscle cells. Am J Pathol. 1973 Oct;73(1):131–146. [PMC free article] [PubMed] [Google Scholar]
  12. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MAJNO G., PALADE G. E. Studies on inflammation. 1. The effect of histamine and serotonin on vascular permeability: an electron microscopic study. J Biophys Biochem Cytol. 1961 Dec;11:571–605. doi: 10.1083/jcb.11.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARCHESI V. T., BARRNETT R. J. The demonstration of enzymatic activity in pinocytic vesicles of blood capillaries with the electron microscope. J Cell Biol. 1963 Jun;17:547–556. doi: 10.1083/jcb.17.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marchesi V. T. The role of pinocytic vesicles in the transport of materials across the walls of small blood vessels. Invest Ophthalmol. 1965 Dec;4(6):1111–1121. [PubMed] [Google Scholar]
  16. McDonald L. W., Hayes T. L. The role of capillaries in the pathogenesis of delayed radionecrosis of brain. Am J Pathol. 1967 May;50(5):745–764. [PMC free article] [PubMed] [Google Scholar]
  17. Phillips S. J., Macken D. L., Rugh R. Pathologic sequelae of acute cardiac irradiation in monkeys. Am Heart J. 1971 Apr;81(4):528–542. doi: 10.1016/0002-8703(71)90370-x. [DOI] [PubMed] [Google Scholar]
  18. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SHIMAMOTO T., ISHIOKA T. Release of a thromboplastic substance from arterial walls by epinephrine. Circ Res. 1963 Feb;12:138–144. doi: 10.1161/01.res.12.2.138. [DOI] [PubMed] [Google Scholar]
  20. Stearner S. P., Sanderson M. H. Early vascular injury in the x-irradiated chick embryo: an electron-microscope study. J Pathol. 1969 Nov;99(3):213–218. doi: 10.1002/path.1710990305. [DOI] [PubMed] [Google Scholar]
  21. Willms-Kretschmer K., Majno G. Ischemia of the skin. Electron microscopic study of vascular injury. Am J Pathol. 1969 Mar;54(3):327–353. [PMC free article] [PubMed] [Google Scholar]

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