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. 1974 Feb;74(2):275–286.

Separation of Cells Having Histochemically Demonstrable Glucose-6-Phosphatase from Suspensions of Hamster Kidney Cells in an Isokinetic Density Gradient of Ficoll in Tissue Culture Medium

Thomas G Pretlow II, Judith Jones, Sherron Dow
PMCID: PMC1910781  PMID: 4359733

Abstract

Suspensions of enzymatically disaggregated hamster kidney cells were separated primarily according to differences in diameter using velocity sedimentation in a previously described isokinetic density gradient and according to differences in density in an isopycnic density gradient. Cells which contained histochemically demonstrable glucose-6-phosphatase were thought to be cells from proximal tubules and constituted 46.5 ± 14.1% of the cells in the starting sample suspension of disaggregated kidney cells. The purest gradient fractions from experiments using velocity sedimentation contained 98.0 ± 0.6% cells which demonstrated glucose-6-phosphatase activity. More than 99.0% of these cells excluded trypan blue. Isopycnic sedimentation was not an effective means of purifying proximal tubule cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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