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. 1974 Apr;75(1):139–160.

Experimental Interactions of Components of Hemodialysis Units with Human Blood

W H Zucker, B A Shinoda, R G Mason
PMCID: PMC1910818  PMID: 4825611

Abstract

An in vitro model test system for estimation of the blood compatibility of hemodialysis membranes and tubing is described. The model test system consists of a modified hemodialysis unit and blood pump through which fresh citrated human blood is circulated. The effects of the use of different pump and tubing types upon hematologic and blood coagulation parameters are described. Preexposure of test surfaces to albumin appeared to enhance blood compatibility characteristics of the model test system, whereas preexposure to a high density lipoprotein preparation or a proteinpolysaccharide preparation was without appreciable benefit. Use of blood from subjects receiving aspirin resulted in enhanced blood compatibility in the test system as did use of heparin. Use of Warfarin or dextran did not appear to enhance blood compatibility of test surfaces under the conditions of this test system. Dialysis membranes and tubing which formed parts of the test system were examined by scanning and transmission electron microscopy in control tests and in tests for effects of proteins and antithrombotic agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahearn D. J., Marshall J. W., Nothum R. J., Esterly J. A., Nolph K. D., Maher J. F. Morphologic studies of dialysis membranes adherence of blood components to air rinsed coils. Trans Am Soc Artif Intern Organs. 1973;19:435–439. doi: 10.1097/00002480-197301900-00075. [DOI] [PubMed] [Google Scholar]
  2. Breen F. A., Jr, Tullis J. L. Ethanol gelation: a rapid screening test for intravascular coagulation. Ann Intern Med. 1968 Dec;69(6):1197–1206. doi: 10.7326/0003-4819-69-6-1197. [DOI] [PubMed] [Google Scholar]
  3. Dhall D. P., Harper D. R., McKenzie F. N., Matheson N. A. Aggregation of human platelets by dextrans. Nature. 1966 May 14;210(5037):745–746. doi: 10.1038/210745b0. [DOI] [PubMed] [Google Scholar]
  4. Fisher B. S., Higley W. S., Cantor P. A., Stone W., Jr Modified polycarbonate membranes for hemodialysis. Trans Am Soc Artif Intern Organs. 1973;19:429–434. doi: 10.1097/00002480-197301900-00074. [DOI] [PubMed] [Google Scholar]
  5. Gotch F., Lipps B., Weaver J., Jr, Brandes J., Rosin J., Sargent J., Oja P. Chronic hemodialysis with the hollow fiber artificial kidney (HFAK). Trans Am Soc Artif Intern Organs. 1969;15:87–96. [PubMed] [Google Scholar]
  6. Gurewich V., Hutchinson E. Detection of intravascular coagulation by a serial-dilution protamine sulfate test. Ann Intern Med. 1971 Dec;75(6):895–902. doi: 10.7326/0003-4819-75-6-895. [DOI] [PubMed] [Google Scholar]
  7. Hawiger J., Niewiarowski S., Gurewich V., Thomas D. P. Measurement of fibrinogen and fibrin degradation products in serum by staphylococcal clumping test. J Lab Clin Med. 1970 Jan;75(1):93–108. [PubMed] [Google Scholar]
  8. Hody G. L., Breeden J. A. Improving the accuracy of a rapid technique of plasma hemoglobin measurement. Am J Med Technol. 1965 Sep-Oct;31(5):367–371. [PubMed] [Google Scholar]
  9. Hutt E. D., Kingdon H. S. Use of heparinase to eliminate heparin inhibition in routine coagulation assays. J Lab Clin Med. 1972 Jun;79(6):1027–1034. [PubMed] [Google Scholar]
  10. Jenkins C. S., Packham M. A., Guccione M. A., Mustard J. F. Modification of platelet adherence to protein-coated surfaces. J Lab Clin Med. 1973 Feb;81(2):280–290. [PubMed] [Google Scholar]
  11. Jensen D. P., Brubaker L. H., Nolph K. D., Johnson C. A., Nothum R. J. Hemodialysis coil-induced transient neutropenia and overshoot neutrophilia in normal man. Blood. 1973 Mar;41(3):399–408. [PubMed] [Google Scholar]
  12. Kalab M., Martin W. G. Electrophoresis of pig serum lipoproteins in agarose gel. Anal Biochem. 1968 Aug;24(2):218–225. doi: 10.1016/0003-2697(68)90174-7. [DOI] [PubMed] [Google Scholar]
  13. LANGDELL R. D., WAGNER R. H., BRINKHOUS K. M. Effect of antihemophilic factor on one-stage clotting tests; a presumptive test for hemophilia and a simple one-stage antihemophilic factor assy procedure. J Lab Clin Med. 1953 Apr;41(4):637–647. [PubMed] [Google Scholar]
  14. Lindsay R. M., Ferguson D., Prentice C. R., Burton J. A., McNicol G. P. Reduction of thrombus formation on dialyser membranes by aspirin and RA 233. Lancet. 1972 Dec 16;2(7790):1287–1290. doi: 10.1016/s0140-6736(72)92656-6. [DOI] [PubMed] [Google Scholar]
  15. Lindsay R. M., Prentice C. R., Davidson J. F., Burton J. A., McNicol G. P. Haemostatic changes during dialysis associated with thrombus formation on dialysis membranes. Br Med J. 1972 Nov 25;4(5838):454–458. doi: 10.1136/bmj.4.5838.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindsay R. M., Prentice C. R., Ferguson D., Muir W. M., McNicol G. P. A method for the measurement of platelet adhesiveness by use of dialysis membranes in a test-cell. Br J Haematol. 1973 Mar;24(3):377–389. doi: 10.1111/j.1365-2141.1973.tb01661.x. [DOI] [PubMed] [Google Scholar]
  17. Lyman B., Rosenberg L., Karpatkin S. Biochemical and biophysical aspects of human platelet adhesion to collagen fibers. J Clin Invest. 1971 Sep;50(9):1854–1863. doi: 10.1172/JCI106677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mason R. G. Blood compatibility of biomaterials: evaluation of a simple screening test. Biomater Med Devices Artif Organs. 1973;1(1):131–139. doi: 10.3109/10731197309118867. [DOI] [PubMed] [Google Scholar]
  19. Mason R. G., Shermer R. W., Rodman N. F. Reactions of blood with nonbiologic surfaces. Ultrastructural and clotting studies with normal and coagulation factor deficient bloods. Am J Pathol. 1972 Nov;69(2):271–288. [PMC free article] [PubMed] [Google Scholar]
  20. Mason R. G., Shermer R. W., Zucker W. H. Effects of certain purified plasma proteins on the compatibility of glass with blood. Am J Pathol. 1973 Oct;73(1):183–200. [PMC free article] [PubMed] [Google Scholar]
  21. Mason R. G. Some methods for in vitro estimation of the blood compatibility of biomaterials. Bull N Y Acad Med. 1972 Feb;48(2):407–424. [PMC free article] [PubMed] [Google Scholar]
  22. Mason R. G. The interaction of blood hemostitic elements with aritificial surfaces. Prog Hemost Thromb. 1972;1:141–164. [PubMed] [Google Scholar]
  23. Mustard J. F., Packham M. A. Factors influencing platelet function: adhesion, release, and aggregation. Pharmacol Rev. 1970 Jun;22(2):97–187. [PubMed] [Google Scholar]
  24. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rosenberg L., Pal S., Beale R., Schubert M. A comparison of proteinpolysaccharides of bovine nasal cartilage isolated and fractionated by different methods. J Biol Chem. 1970 Aug 25;245(16):4112–4122. [PubMed] [Google Scholar]
  26. Schiffman S., Rapaport S. I., Chong M. M. Platelets and initiation of intrinsic clotting. Br J Haematol. 1973 May;24(5):633–642. doi: 10.1111/j.1365-2141.1973.tb01689.x. [DOI] [PubMed] [Google Scholar]
  27. Walsh P. N., Biggs R. The role of platelets in intrinsic factor-Xa formation. Br J Haematol. 1972 Jun;22(6):743–760. doi: 10.1111/j.1365-2141.1972.tb05719.x. [DOI] [PubMed] [Google Scholar]

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