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. 1974 May;75(2):329–348.

Pancreatic Beta Cell Replication Induced by Glucocorticoids in Subhuman Primates

A A Like, W L Chick
PMCID: PMC1910838  PMID: 4207270

Abstract

Pancreatic islets were studied by means of light microsocpy, autoradiography and electron microscopy in untreated Macaca cyclopis monkeys and after the administration of large quantities of adrenal glucocorticoids. Mild hyperglycemia and profound elevations of serum immunoreactive insulin were induced by glucocorticoid injections of 1 to 3 weeks duration, with a gradual return to pretreatment levels within 2 months after cessation of treatment. Morphologic alterations included degranulation and hyperplasia of pancreatic beta cells. These were noted in association with increased numbers of labeled islet cells after the administration of 3H-thymidine and beta cells undergoing mitotic division, and could be correlated directly with the magnitude of serum insulin elevation. Evidence of acinar-islet or duct-islet cell transformation was absent. Beta cell regranulation and the twofold increase in extractable pancreatic insulin which followed the cessation of injections demonstrated the survival and functional integrity of the newly formed beta cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABELOVE W. A., PASCHKIS K. E. Comparison of the diabetogenic action of cortisone and growth hormone in different species. Endocrinology. 1954 Nov;55(5):637–654. doi: 10.1210/endo-55-5-637. [DOI] [PubMed] [Google Scholar]
  2. BLEISCH V. R., MAYER J., DICKIE M. M. Familial diabetes mellitus in mice, associated with insulin resistance, obesity, and hyperplasia of the islands of langerhans. Am J Pathol. 1952 May-Jun;28(3):369–385. [PMC free article] [PubMed] [Google Scholar]
  3. CAVALLERO C., MOSCA L. Mitotic activity in the pancreatic islets of the rat under pituitary growth hormone and adrenocorticotropic hormone treatment. J Pathol Bacteriol. 1953 Jul;66(1):147–150. doi: 10.1002/path.1700660118. [DOI] [PubMed] [Google Scholar]
  4. Carpenter A. M., Gerritsen G. C., Dulin W. E., Lazarow A. Islet and beta cell volumes in diabetic Chinese hamsters and their non-diabetic siblings. Diabetologia. 1967 Apr;3(2):92–96. doi: 10.1007/BF01222184. [DOI] [PubMed] [Google Scholar]
  5. Carpenter A. M., Gerritsen G. C., Dulin W. E., Lazarow A. Islet and beta cell volumes in offspring of severely diabetic (ketotic) chinese hamsters. Diabetologia. 1970 Jun;6(3):168–176. doi: 10.1007/BF01212225. [DOI] [PubMed] [Google Scholar]
  6. Chick W. L., Like A. A. Studies in the diabetic mutant mouse. 3. Physiological factors associated with alterations in beta cell proliferation. Diabetologia. 1970 Jun;6(3):243–251. doi: 10.1007/BF01212233. [DOI] [PubMed] [Google Scholar]
  7. Coleman D. L., Hummel K. P. Studies with the mutation, diabetes, in the mouse. Diabetologia. 1967 Apr;3(2):238–248. doi: 10.1007/BF01222201. [DOI] [PubMed] [Google Scholar]
  8. Coleman D. L., Hummel K. P. The influence of genetic background on the expression of the obese (Ob) gene in the mouse. Diabetologia. 1973 Aug;9(4):287–293. doi: 10.1007/BF01221856. [DOI] [PubMed] [Google Scholar]
  9. DAVOREN P. R. The isolation of insulin from a single cat pancreas. Biochim Biophys Acta. 1962 Sep 10;63:150–153. doi: 10.1016/0006-3002(62)90347-5. [DOI] [PubMed] [Google Scholar]
  10. FRANCKSON J. R., GEPTS W., BASTENIE P. A., CONARD V., CORDIER N., KOVACS L. Observations sur le diabete steroide experimental du rat. Acta Endocrinol (Copenh) 1953 Oct;14(2):153–169. [PubMed] [Google Scholar]
  11. Gepts W. Pathologic anatomy of the pancreas in juvenile diabetes mellitus. Diabetes. 1965 Oct;14(10):619–633. doi: 10.2337/diab.14.10.619. [DOI] [PubMed] [Google Scholar]
  12. Gleason R. E., Lauris V., Soeldner J. S. Studies on experimental diabetes in the Wellesley hybrid mouse. 3. Dietary effects and similar changes in a commercial Swiss-Hauschka strain. Diabetologia. 1967 Apr;3(2):175–178. doi: 10.1007/BF01222195. [DOI] [PubMed] [Google Scholar]
  13. HAUSBERGER F. X., RAMSAY A. J. Islet hypertrophy in obesity of mice bearing ACTH-secreting tumors. Endocrinology. 1959 Aug;65(2):165–171. doi: 10.1210/endo-65-2-165. [DOI] [PubMed] [Google Scholar]
  14. HAUSBERGER F. X., RAMSAY A. J. Steroid diabetes in guinea pigs; effects of cortisone administration on blood- and urinary glucose, nitrogen excretion, fat deposition, and the islets of Langerhans. Endocrinology. 1953 Oct;53(4):423–435. doi: 10.1210/endo-53-4-423. [DOI] [PubMed] [Google Scholar]
  15. Hummel K. P., Dickie M. M., Coleman D. L. Diabetes, a new mutation in the mouse. Science. 1966 Sep 2;153(3740):1127–1128. doi: 10.1126/science.153.3740.1127. [DOI] [PubMed] [Google Scholar]
  16. Kern H., Logothetopoulos J. Steroid diabetes in the guinea pig. Studies on islet-cell ultrastructure and regeneration. Diabetes. 1970 Mar;19(3):145–154. doi: 10.2337/diab.19.3.145. [DOI] [PubMed] [Google Scholar]
  17. LAZARUS S. S., BENCOSME S. A. Alterations of pancreas during cortisone diabetes in rabbits. Proc Soc Exp Biol Med. 1955 May;89(1):114–118. doi: 10.3181/00379727-89-21731. [DOI] [PubMed] [Google Scholar]
  18. Like A. A., Chick W. L. Mitotic division in pancreatic beta cells. Science. 1969 Feb 28;163(3870):941–943. doi: 10.1126/science.163.3870.941. [DOI] [PubMed] [Google Scholar]
  19. Like A. A., Chick W. L. Studies in the diabetic mutant mouse. I. Light microscopy and radioautography of pancreatic islets. Diabetologia. 1970 Jun;6(3):207–215. doi: 10.1007/BF01212231. [DOI] [PubMed] [Google Scholar]
  20. Like A. A., Chick W. L. Studies in the diabetic mutant mouse. II. Electron microscopy of pancreatic islets. Diabetologia. 1970 Jun;6(3):216–242. doi: 10.1007/BF01212232. [DOI] [PubMed] [Google Scholar]
  21. Like A. A., Jones E. E. Studies on experimental diabetes in the Wellesley hybrid mouse. IV. Morphologic changes in islet tissue. Diabetologia. 1967 Apr;3(2):179–187. doi: 10.1007/BF01222196. [DOI] [PubMed] [Google Scholar]
  22. Like A. A., Miki E. Diabetic syndrome in sand rats. IV. Morphologic changes in islet tissue. Diabetologia. 1967 Apr;3(2):143–166. doi: 10.1007/BF01222192. [DOI] [PubMed] [Google Scholar]
  23. Like A. A. The ultrastructure of the secretory cells of the islets of Langerhans in man. Lab Invest. 1967 Jun;16(6):937–951. [PubMed] [Google Scholar]
  24. Logothetopoulos J., Bell E. G. Histological and autoradiographic studies of the islets of mice injected with insulin antibody. Diabetes. 1966 Mar;15(3):205–211. doi: 10.2337/diab.15.3.205. [DOI] [PubMed] [Google Scholar]
  25. MEIER H., YERGANIAN G. A. Spontaneous hereditary diabetes mellitus in Chinese hamster (Cricetulus griseus). 1. Pathological findings. Proc Soc Exp Biol Med. 1959 Apr;100(4):810–815. doi: 10.3181/00379727-100-24786. [DOI] [PubMed] [Google Scholar]
  26. PAGET G. E. Aldehyde-thionin: a stain having similar properties to aldehydefuchsin. Stain Technol. 1959 Jul;34(4):223–226. doi: 10.3109/10520295909114679. [DOI] [PubMed] [Google Scholar]
  27. RIVIERE M., COMBESCOT C. Sur le conditionnement de certaines transformations langerhansiennes chez le singe. C R Seances Soc Biol Fil. 1954 Jan;148(1-2):93–95. [PubMed] [Google Scholar]
  28. SCHMIDT-NIELSEN K., HAINES H. B., HACKEL D. B. DIABETES MELLITUS IN THE SAND RAT INDUCED BY STANDARD LABORATORY DIETS. Science. 1964 Feb 14;143(3607):689–690. doi: 10.1126/science.143.3607.689. [DOI] [PubMed] [Google Scholar]
  29. Scott D. A., Fisher A. M. THE INSULIN AND THE ZINC CONTENT OF NORMAL AND DIABETIC PANCREAS. J Clin Invest. 1938 Nov;17(6):725–728. doi: 10.1172/JCI101000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Soeldner J. S., Slone D. Critical variables in the radioimmunoassay of serum insulin using the double antibody technic. Diabetes. 1965 Dec;14(12):771–779. doi: 10.2337/diab.14.12.771. [DOI] [PubMed] [Google Scholar]
  31. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. WRENSHALL G. A., BOGOCH A., RITCHIE R. C. Extractable insulin of pancreas; correlation with pathological and clinical findings in diabetic and nondiabetic cases. Diabetes. 1952 Mar-Apr;1(2):87–107. doi: 10.2337/diab.1.2.87. [DOI] [PubMed] [Google Scholar]

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