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. 1997 Jan;71(1):738–740. doi: 10.1128/jvi.71.1.738-740.1997

Fusion of bovine leukemia virus with target cells monitored by R18 fluorescence and PCR assays.

S Zarkik 1, F Defrise-Quertain 1, D Portetelle 1, A Burny 1, J M Ruysschaert 1
PMCID: PMC191109  PMID: 8985408

Abstract

PCR and R18 fluorescence dequenching assays have been combined to monitor the kinetics of fusion of bovine leukemia virus with target cells (CC81, OVK, or Raji). Antibodies raised against gp51 allow us to demonstrate that not only the hydrophobic N-terminal domain of the transmembrane glycoprotein gp30 but also specific domains of gp51 (amino acids 39 to 103) are involved in bovine leukemia virus-cell fusion.

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Selected References

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