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. 1997 Mar;71(3):2478–2481. doi: 10.1128/jvi.71.3.2478-2481.1997

Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient.

J Koide 1, K Takada 1, M Sugiura 1, H Sekine 1, T Ito 1, K Saito 1, S Mori 1, T Takeuchi 1, S Uchida 1, T Abe 1
PMCID: PMC191359  PMID: 9032386

Abstract

An Epstein-Barr virus (EBV)-infected fibroblast line, designated DSEK, was spontaneously established from synovial tissue of a patient with rheumatoid arthritis (RA). DSEK cells expressed EBV nuclear antigens EBNA-1 and EBNA-2 and latent membrane protein LMP-1. Cell surface markers of DSEK cells were similar to those of EBV-negative fibroblast clones derived from synoviocytes and were negative for lymphocyte and macrophage markers. DSEK cells expressed CD44, CD58, and HLA-DR antigens and spontaneously produced interleukin-10 basic fibroblast growth factor and transforming growth factor beta1. These results indicate that rheumatoid synoviocytes can be a target for EBV infection and suggest that EBV may play a role in the pathogenesis of RA.

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Selected References

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