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. 1997 Mar;71(3):2562–2566. doi: 10.1128/jvi.71.3.2562-2566.1997

Mapping and characterization of the origin of DNA replication of porcine circovirus.

A Mankertz 1, F Persson 1, J Mankertz 1, G Blaess 1, H J Buhk 1
PMCID: PMC191374  PMID: 9032401

Abstract

The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.

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Selected References

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  1. Boevink P., Chu P. W., Keese P. Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology. 1995 Mar 10;207(2):354–361. doi: 10.1006/viro.1995.1094. [DOI] [PubMed] [Google Scholar]
  2. Claessens J. A., Schrier C. C., Mockett A. P., Jagt E. H., Sondermeijer P. J. Molecular cloning and sequence analysis of the genome of chicken anaemia agent. J Gen Virol. 1991 Aug;72(Pt 8):2003–2006. doi: 10.1099/0022-1317-72-8-2003. [DOI] [PubMed] [Google Scholar]
  3. DeLange A. M., McFadden G. Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells. Proc Natl Acad Sci U S A. 1986 Feb;83(3):614–618. doi: 10.1073/pnas.83.3.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dulac G. C., Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can J Vet Res. 1989 Oct;53(4):431–433. [PMC free article] [PubMed] [Google Scholar]
  5. Fontes E. P., Eagle P. A., Sipe P. S., Luckow V. A., Hanley-Bowdoin L. Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. J Biol Chem. 1994 Mar 18;269(11):8459–8465. [PubMed] [Google Scholar]
  6. Hanson S. F., Hoogstraten R. A., Ahlquist P., Gilbertson R. L., Russell D. R., Maxwell D. P. Mutational analysis of a putative NTP-binding domain in the replication-associated protein (AC1) of bean golden mosaic geminivirus. Virology. 1995 Aug 1;211(1):1–9. doi: 10.1006/viro.1995.1373. [DOI] [PubMed] [Google Scholar]
  7. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. J Gen Virol. 1993 Mar;74(Pt 3):323–328. doi: 10.1099/0022-1317-74-3-323. [DOI] [PubMed] [Google Scholar]
  8. Heidekamp F., Baas P. D., Jansz H. S. Nucleotide sequences at the phi X gene A protein cleavage site in replicative form I DNAs of bacteriophages U3, G14, and alpha 3. J Virol. 1982 Apr;42(1):91–99. doi: 10.1128/jvi.42.1.91-99.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heyraud-Nitschke F., Schumacher S., Laufs J., Schaefer S., Schell J., Gronenborn B. Determination of the origin cleavage and joining domain of geminivirus Rep proteins. Nucleic Acids Res. 1995 Mar 25;23(6):910–916. doi: 10.1093/nar/23.6.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heyraud F., Matzeit V., Kammann M., Schaefer S., Schell J., Gronenborn B. Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO J. 1993 Nov;12(11):4445–4452. doi: 10.1002/j.1460-2075.1993.tb06130.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  12. Koonin E. V., Ilyina T. V. Computer-assisted dissection of rolling circle DNA replication. Biosystems. 1993;30(1-3):241–268. doi: 10.1016/0303-2647(93)90074-m. [DOI] [PubMed] [Google Scholar]
  13. Laufs J., Traut W., Heyraud F., Matzeit V., Rogers S. G., Schell J., Gronenborn B. In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3879–3883. doi: 10.1073/pnas.92.9.3879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lazarowitz S. G., Wu L. C., Rogers S. G., Elmer J. S. Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin. Plant Cell. 1992 Jul;4(7):799–809. doi: 10.1105/tpc.4.7.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Michel B., Ehrlich S. D. Illegitimate recombination occurs between the replication origin of the plasmid pC194 and a progressing replication fork. EMBO J. 1986 Dec 20;5(13):3691–3696. doi: 10.1002/j.1460-2075.1986.tb04701.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mullineaux P. M., Donson J., Morris-Krsinich B. A., Boulton M. I., Davies J. W. The nucleotide sequence of maize streak virus DNA. EMBO J. 1984 Dec 20;3(13):3063–3068. doi: 10.1002/j.1460-2075.1984.tb02258.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Orozco B. M., Hanley-Bowdoin L. A DNA structure is required for geminivirus replication origin function. J Virol. 1996 Jan;70(1):148–158. doi: 10.1128/jvi.70.1.148-158.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Revington G. N., Sunter G., Bisaro D. M. DNA sequences essential for replication of the B genome component of tomato golden mosaic virus. Plant Cell. 1989 Oct;1(10):985–992. doi: 10.1105/tpc.1.10.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ritchie B. W., Niagro F. D., Lukert P. D., Steffens W. L., 3rd, Latimer K. S. Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology. 1989 Jul;171(1):83–88. doi: 10.1016/0042-6822(89)90513-8. [DOI] [PubMed] [Google Scholar]
  20. Rohde W., Randles J. W., Langridge P., Hanold D. Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology. 1990 Jun;176(2):648–651. doi: 10.1016/0042-6822(90)90038-s. [DOI] [PubMed] [Google Scholar]
  21. Stanley J. Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle DNA replication. Virology. 1995 Jan 10;206(1):707–712. doi: 10.1016/s0042-6822(95)80093-x. [DOI] [PubMed] [Google Scholar]
  22. Stenger D. C., Revington G. N., Stevenson M. C., Bisaro D. M. Replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling-circle replication of a plant viral DNA. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8029–8033. doi: 10.1073/pnas.88.18.8029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Studdert M. J. Circoviridae: new viruses of pigs, parrots and chickens. Aust Vet J. 1993 Apr;70(4):121–122. doi: 10.1111/j.1751-0813.1993.tb06100.x. [DOI] [PubMed] [Google Scholar]
  24. Tischer I., Bode L., Peters D., Pociuli S., Germann B. Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Arch Virol. 1995;140(4):737–743. doi: 10.1007/BF01309961. [DOI] [PubMed] [Google Scholar]
  25. Tischer I., Buhk H. J. Viral DNA from cells infected with porcine circovirus. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;270(1-2):280–287. doi: 10.1016/s0176-6724(88)80164-0. [DOI] [PubMed] [Google Scholar]
  26. Tischer I., Gelderblom H., Vettermann W., Koch M. A. A very small porcine virus with circular single-stranded DNA. Nature. 1982 Jan 7;295(5844):64–66. doi: 10.1038/295064a0. [DOI] [PubMed] [Google Scholar]
  27. Tischer I., Mields W., Wolff D., Vagt M., Griem W. Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol. 1986;91(3-4):271–276. doi: 10.1007/BF01314286. [DOI] [PubMed] [Google Scholar]
  28. Tischer I., Peters D., Rasch R., Pociuli S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol. 1987;96(1-2):39–57. doi: 10.1007/BF01310989. [DOI] [PubMed] [Google Scholar]
  29. Tischer I., Rasch R., Tochtermann G. Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Orig A. 1974 Feb;226(2):153–167. [PubMed] [Google Scholar]

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