Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1997 Apr;71(4):3279–3284. doi: 10.1128/jvi.71.4.3279-3284.1997

Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7.

N van Loon 1, C Dykes 1, H Deng 1, G Dominguez 1, J Nicholas 1, S Dewhurst 1
PMCID: PMC191464  PMID: 9060695

Abstract

Human herpesvirus 7 (HHV-7) DNA sequences colinear with the HHV-6 lytic-phase origin of DNA replication (oriLyt) were amplified by PCR. Plasmid constructs containing these sequences were replicated in HHV-7-infected cord blood mononuclear cells but not in HHV-6-infected cells. In contrast, plasmids bearing HHV-6 oriLyt were replicated in both HHV-6- and HHV-7-infected cells. Finally, the minimal HHV-7 DNA element necessary for replicator activity was mapped to a 600-bp region which contains two sites with high homology to the consensus binding site for the HHV-6 origin binding protein. At least one of these binding sites was shown to be essential for replicator function of HHV-7 oriLyt.

Full Text

The Full Text of this article is available as a PDF (682.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders D. G., Punturieri S. M. Multicomponent origin of cytomegalovirus lytic-phase DNA replication. J Virol. 1991 Feb;65(2):931–937. doi: 10.1128/jvi.65.2.931-937.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asano Y., Suga S., Yoshikawa T., Yazaki T., Uchikawa T. Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection. Pediatrics. 1995 Feb;95(2):187–190. [PubMed] [Google Scholar]
  3. Cermelli C., Fabio G., Montorsi M., Sabbatini A. M., Portolani M. Prevalence of antibodies to human herpesviruses 6 and 7 in early infancy and age at primary infection. New Microbiol. 1996 Jan;19(1):1–8. [PubMed] [Google Scholar]
  4. Challoner P. B., Smith K. T., Parker J. D., MacLeod D. L., Coulter S. N., Rose T. M., Schultz E. R., Bennett J. L., Garber R. L., Chang M. Plaque-associated expression of human herpesvirus 6 in multiple sclerosis. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7440–7444. doi: 10.1073/pnas.92.16.7440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cone R. W., Huang M. L., Hackman R. C. Human herpesvirus 6 and pneumonia. Leuk Lymphoma. 1994 Oct;15(3-4):235–241. doi: 10.3109/10428199409049719. [DOI] [PubMed] [Google Scholar]
  6. Crowley R. W., Secchiero P., Zella D., Cara A., Gallo R. C., Lusso P. Interference between human herpesvirus 7 and HIV-1 in mononuclear phagocytes. J Immunol. 1996 Mar 1;156(5):2004–2008. [PubMed] [Google Scholar]
  7. Dabrowski C. E., Carmillo P. J., Schaffer P. A. Cellular protein interactions with herpes simplex virus type 1 oriS. Mol Cell Biol. 1994 Apr;14(4):2545–2555. doi: 10.1128/mcb.14.4.2545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dewhurst S., Dollard S. C., Pellett P. E., Dambaugh T. R. Identification of a lytic-phase origin of DNA replication in human herpesvirus 6B strain Z29. J Virol. 1993 Dec;67(12):7680–7683. doi: 10.1128/jvi.67.12.7680-7683.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dewhurst S., Krenitsky D. M., Dykes C. Human herpesvirus 6B origin: sequence diversity, requirement for two binding sites for origin-binding protein, and enhanced replication from origin multimers. J Virol. 1994 Oct;68(10):6799–6803. doi: 10.1128/jvi.68.10.6799-6803.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Drobyski W. R., Dunne W. M., Burd E. M., Knox K. K., Ash R. C., Horowitz M. M., Flomenberg N., Carrigan D. R. Human herpesvirus-6 (HHV-6) infection in allogeneic bone marrow transplant recipients: evidence of a marrow-suppressive role for HHV-6 in vivo. J Infect Dis. 1993 Mar;167(3):735–739. doi: 10.1093/infdis/167.3.735. [DOI] [PubMed] [Google Scholar]
  11. Drobyski W. R., Knox K. K., Majewski D., Carrigan D. R. Brief report: fatal encephalitis due to variant B human herpesvirus-6 infection in a bone marrow-transplant recipient. N Engl J Med. 1994 May 12;330(19):1356–1360. doi: 10.1056/NEJM199405123301905. [DOI] [PubMed] [Google Scholar]
  12. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. Isolation of a new herpesvirus from human CD4+ T cells. Proc Natl Acad Sci U S A. 1990 Jan;87(2):748–752. doi: 10.1073/pnas.87.2.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Frenkel N., Wyatt L. S. HHV-6 and HHV-7 as exogenous agents in human lymphocytes. Dev Biol Stand. 1992;76:259–265. [PubMed] [Google Scholar]
  14. Geller A. I., Breakefield X. O. A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons. Science. 1988 Sep 23;241(4873):1667–1669. doi: 10.1126/science.241.4873.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hall C. B., Long C. E., Schnabel K. C., Caserta M. T., McIntyre K. M., Costanzo M. A., Knott A., Dewhurst S., Insel R. A., Epstein L. G. Human herpesvirus-6 infection in children. A prospective study of complications and reactivation. N Engl J Med. 1994 Aug 18;331(7):432–438. doi: 10.1056/NEJM199408183310703. [DOI] [PubMed] [Google Scholar]
  16. Hamzeh F. M., Lietman P. S., Gibson W., Hayward G. S. Identification of the lytic origin of DNA replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination. J Virol. 1990 Dec;64(12):6184–6195. doi: 10.1128/jvi.64.12.6184-6195.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hidaka Y., Okada K., Kusuhara K., Miyazaki C., Tokugawa K., Ueda K. Exanthem subitum and human herpesvirus 7 infection. Pediatr Infect Dis J. 1994 Nov;13(11):1010–1011. [PubMed] [Google Scholar]
  18. Inoue N., Dambaugh T. R., Rapp J. C., Pellett P. E. Alphaherpesvirus origin-binding protein homolog encoded by human herpesvirus 6B, a betaherpesvirus, binds to nucleotide sequences that are similar to ori regions of alphaherpesviruses. J Virol. 1994 Jul;68(7):4126–4136. doi: 10.1128/jvi.68.7.4126-4136.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Inoue N., Pellett P. E. Human herpesvirus 6B origin-binding protein: DNA-binding domain and consensus binding sequence. J Virol. 1995 Aug;69(8):4619–4627. doi: 10.1128/jvi.69.8.4619-4627.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jones C. M., Dunn H. G., Thomas E. E., Cone R. W., Weber J. M. Acute encephalopathy and status epilepticus associated with human herpes virus 6 infection. Dev Med Child Neurol. 1994 Jul;36(7):646–650. doi: 10.1111/j.1469-8749.1994.tb11903.x. [DOI] [PubMed] [Google Scholar]
  21. Katsafanas G. C., Schirmer E. C., Wyatt L. S., Frenkel N. In vitro activation of human herpesviruses 6 and 7 from latency. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9788–9792. doi: 10.1073/pnas.93.18.9788. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kilwinski J., Baack M., Heiland S., Knippers R. Transcription factor Oct1 binds to the AT-rich segment of the simian virus 40 replication origin. J Virol. 1995 Jan;69(1):575–578. doi: 10.1128/jvi.69.1.575-578.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Knox K. K., Harrington D. P., Carrigan D. R. Fulminant human herpesvirus six encephalitis in a human immunodeficiency virus-infected infant. J Med Virol. 1995 Mar;45(3):288–292. doi: 10.1002/jmv.1890450309. [DOI] [PubMed] [Google Scholar]
  24. Lusso P., De Maria A., Malnati M., Lori F., DeRocco S. E., Baseler M., Gallo R. C. Induction of CD4 and susceptibility to HIV-1 infection in human CD8+ T lymphocytes by human herpesvirus 6. Nature. 1991 Feb 7;349(6309):533–535. doi: 10.1038/349533a0. [DOI] [PubMed] [Google Scholar]
  25. Lusso P., Gallo R. C. Human herpesvirus 6 in AIDS. Immunol Today. 1995 Feb;16(2):67–71. doi: 10.1016/0167-5699(95)80090-5. [DOI] [PubMed] [Google Scholar]
  26. Lusso P., Garzino-Demo A., Crowley R. W., Malnati M. S. Infection of gamma/delta T lymphocytes by human herpesvirus 6: transcriptional induction of CD4 and susceptibility to HIV infection. J Exp Med. 1995 Apr 1;181(4):1303–1310. doi: 10.1084/jem.181.4.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Lusso P., Malnati M. S., Garzino-Demo A., Crowley R. W., Long E. O., Gallo R. C. Infection of natural killer cells by human herpesvirus 6. Nature. 1993 Apr 1;362(6419):458–462. doi: 10.1038/362458a0. [DOI] [PubMed] [Google Scholar]
  28. Lusso P., Secchiero P., Crowley R. W., Garzino-Demo A., Berneman Z. N., Gallo R. C. CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3872–3876. doi: 10.1073/pnas.91.9.3872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. McCullers J. A., Lakeman F. D., Whitley R. J. Human herpesvirus 6 is associated with focal encephalitis. Clin Infect Dis. 1995 Sep;21(3):571–576. doi: 10.1093/clinids/21.3.571. [DOI] [PubMed] [Google Scholar]
  30. Natale D. A., Schubert A. E., Kowalski D. DNA helical stability accounts for mutational defects in a yeast replication origin. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2654–2658. doi: 10.1073/pnas.89.7.2654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Nicholas J. Determination and analysis of the complete nucleotide sequence of human herpesvirus. J Virol. 1996 Sep;70(9):5975–5989. doi: 10.1128/jvi.70.9.5975-5989.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Portolani M., Cermelli C., Mirandola P., Di Luca D. Isolation of human herpesvirus 7 from an infant with febrile syndrome. J Med Virol. 1995 Mar;45(3):282–283. doi: 10.1002/jmv.1890450307. [DOI] [PubMed] [Google Scholar]
  33. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986 Oct 31;234(4776):596–601. doi: 10.1126/science.2876520. [DOI] [PubMed] [Google Scholar]
  34. Secchiero P., Nicholas J., Deng H., Xiaopeng T., van Loon N., Ruvolo V. R., Berneman Z. N., Reitz M. S., Jr, Dewhurst S. Identification of human telomeric repeat motifs at the genome termini of human herpesvirus 7: structural analysis and heterogeneity. J Virol. 1995 Dec;69(12):8041–8045. doi: 10.1128/jvi.69.12.8041-8045.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Spaete R. R., Frenkel N. The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector. Cell. 1982 Aug;30(1):295–304. doi: 10.1016/0092-8674(82)90035-6. [DOI] [PubMed] [Google Scholar]
  36. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet. 1988 May 14;1(8594):1065–1067. doi: 10.1016/s0140-6736(88)91893-4. [DOI] [PubMed] [Google Scholar]
  37. Yasukawa M., Inoue Y., Sada E., Yakushijin Y., Furukawa M., Fujita S. CD4 down-modulation by ganglioside and phorbol ester inhibits human herpesvirus 7 infection. J Gen Virol. 1995 Sep;76(Pt 9):2381–2385. doi: 10.1099/0022-1317-76-9-2381. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES