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. 1982 Jul;108(1):50–59.

Interactions between macrophagelike cells and Leishmania braziliensis in vitro.

M Aikawa, L D Hendricks, Y Ito, M Jagusiak
PMCID: PMC1916017  PMID: 7091302

Abstract

The interaction between macrophagelike cells (P388D murine tumor cells) and Leishmania braziliensis panamensis promastigotes was studied in vitro under various conditions by light and electron microscopy. When the macrophagelike cells and L braziliensis promastigate were incubated together for 2 minutes, 90% of the promastigotes were attached to the macrophagelike cells by the tip of the flagella. The macrophagelike cells did not form pseudopods or aggregated microfilaments around the inserted flagellum. After a 5-minute incubation period, the parasites attached to the macrophagelike cells did not show preferred orientation. At this time, numerous pseudopods and aggregated microfilaments of the macrophagelike cells were seen around the invading parasites. When the relationship between the cytochalasin B-treated promastigotes and the macrophagelike cells were examined, no interaction of the promastigote flagella with macrophagelike cells was observed at 2 minutes. After a 5-minute incubation period, 50% of the attached parasites adhered to a macrophagelike cell without any particular orientation. When the interaction between the promastigotes and cytochalasin B-treated macrophagelike cells were examined, the cytochalasin B-treated cells showed fewer pseudopods than the untreated cells, and the number of parasites attached to them was reduced considerably after a 5-minute incubation period. This data demonstrated, for the first time, that the mode of entry by Leishmania promastigotes into macrophagelike cells is dependent on the activation of the macrophagelike cells following the attachment of Leishmania promastigotes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama H. J., Haight R. D. Interaction of Leishmania donovani and hamster peritoneal macrophages. A phase-contrast microscopical study. Am J Trop Med Hyg. 1971 Jul;20(4):539–545. doi: 10.4269/ajtmh.1971.20.539. [DOI] [PubMed] [Google Scholar]
  2. Alexander J. Effect of the antiphagocytic agent cytochalasin B on macrophage invasion by Leishmania mexicana promastigotes and Trypanosoma cruzi epimastigotes. J Protozool. 1975 May;22(2):237–240. doi: 10.1111/j.1550-7408.1975.tb05858.x. [DOI] [PubMed] [Google Scholar]
  3. Ardehali S. M., Khoubyar K., Rezai H. R. Studies on the effect of the anti-phagocytic agent cytochalasin B on Leishmania-macrophage interaction. Acta Trop. 1979 Mar;36(1):15–21. [PubMed] [Google Scholar]
  4. Berens R. L., Marr J. J. Growth of Leishmania donovani amastigotes in a continuous macrophage-like cell culture. J Protozool. 1979 Aug;26(3):453–456. doi: 10.1111/j.1550-7408.1979.tb04652.x. [DOI] [PubMed] [Google Scholar]
  5. Chang K. P., Dwyer D. M. Leishmania donovani. Hamster macrophage interactions in vitro: cell entry, intracellular survival, and multiplication of amastigotes. J Exp Med. 1978 Feb 1;147(2):515–530. doi: 10.1084/jem.147.2.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chang K. P. Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay. Mol Biochem Parasitol. 1981 Nov;4(1-2):67–76. doi: 10.1016/0166-6851(81)90030-x. [DOI] [PubMed] [Google Scholar]
  7. Chang K. P. Leishmania donovani: promastigote--macrophage surface interactions in vitro. Exp Parasitol. 1979 Oct;48(2):175–189. doi: 10.1016/0014-4894(79)90097-3. [DOI] [PubMed] [Google Scholar]
  8. Cohn Z. A. Activation of mononuclear phagocytes: fact, fancy, and future. J Immunol. 1978 Sep;121(3):813–816. [PubMed] [Google Scholar]
  9. Hendricks L. D., Wood D. E., Hajduk M. E. Haemoflagellates: commercially available liquid media for rapid cultivation. Parasitology. 1978 Jun;76(3):309–316. doi: 10.1017/s0031182000048186. [DOI] [PubMed] [Google Scholar]
  10. Lewis D. H. Infection of tissue culture cells of low phagocytic ability by Leishmania mexicana mexicana. Ann Trop Med Parasitol. 1974 Sep;68(3):327–336. doi: 10.1080/00034983.1974.11686955. [DOI] [PubMed] [Google Scholar]
  11. Mazur M. T., Williamson J. R. Macrophage deformability and phagocytosis. J Cell Biol. 1977 Oct;75(1):185–199. doi: 10.1083/jcb.75.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McKinney L. A., Hendricks L. D. Experimental infection of Mystromys albicaudatus with Leishmania braziliensis: pathology. Am J Trop Med Hyg. 1980 Sep;29(5):753–760. doi: 10.4269/ajtmh.1980.29.753. [DOI] [PubMed] [Google Scholar]
  13. Merino F., Ajjam E., Hernández A., Dawidowicz K., Merino E. J. In vitro infection of murine macrophage by Leishmania brasiliensis. Mechanism of penetration. Int Arch Allergy Appl Immunol. 1977;55(1-6):487–495. doi: 10.1159/000231962. [DOI] [PubMed] [Google Scholar]
  14. Miller H. C., Twohy D. W. Infection of macrophages in culture by leptomonads of Leishmania donovani. J Protozool. 1967 Nov;14(4):781–789. doi: 10.1111/j.1550-7408.1967.tb02078.x. [DOI] [PubMed] [Google Scholar]
  15. Miller L. H., Aikawa M., Johnson J. G., Shiroishi T. Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation. J Exp Med. 1979 Jan 1;149(1):172–184. doi: 10.1084/jem.149.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PULVERTAFT R. J., HOYLE G. F. Stages in the life-cycle of Leishmania donovani. Trans R Soc Trop Med Hyg. 1960 Mar;54:191–196. doi: 10.1016/0035-9203(60)90057-2. [DOI] [PubMed] [Google Scholar]
  17. Pearson R. D., Romito R., Symes P. H., Harcus J. L. Interaction of Leishmania donovani promastigotes with human monocyte-derived macrophages: parasite entry, intracellular survival, and multiplication. Infect Immun. 1981 Jun;32(3):1249–1253. doi: 10.1128/iai.32.3.1249-1253.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reaven E. P., Axline S. G. Subplasmalemmal microfilaments and microtubules in resting and phagocytizing cultivated macrophages. J Cell Biol. 1973 Oct;59(1):12–27. doi: 10.1083/jcb.59.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zenian A., Rowles P., Gingell D. Scanning electron-microscopic study of the uptake of Leishmania parasites by macrophages. J Cell Sci. 1979 Oct;39:187–199. doi: 10.1242/jcs.39.1.187. [DOI] [PubMed] [Google Scholar]

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