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. 1992 Jul;36(7):1478–1482. doi: 10.1128/aac.36.7.1478

In vitro activity of nonoxynol 9 on HeLa 229 cells and primary monkey cervical epithelial cells infected with Chlamydia trachomatis.

D L Patton 1, S K Wang 1, C C Kuo 1
PMCID: PMC191607  PMID: 1324646

Abstract

Nonoxynol 9 (non-9) is the active ingredient in a wide variety of vaginal contraceptive preparations. The manufacturer recommendation for optimal contraceptive practice is repeated application every 6 h. We studied the in vitro activity of non-9 against Chlamydia trachomatis (E/UW-5/Cx) and its toxicity against HeLa 229 cells and monkey cervical epithelial cells. With a contact time of 6 h, non-9 was toxic to HeLa cells at concentrations of 50 micrograms/ml or greater and to monkey cervical cells at 100 micrograms/ml or greater. Inhibitory effects of non-9 on extracellular chlamydiae were observed at concentrations of 50 micrograms/ml or greater. Inhibition of intracellular growth of chlamydiae in monkey cervical cells was observed at a nontoxic concentration of 50 micrograms/ml. Our studies show that non-9 has antichlamydial activity. However, owing to its toxicity to cervical cells in vitro, the effects of prolonged use of non-9 in vivo should be further studied.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amortegui A. J., Melder R. J., Meyer M. P., Singh B. The effect of chemical intravaginal contraceptives and Betadine on Ureaplasma urealyticum. Contraception. 1984 Aug;30(2):135–141. doi: 10.1016/0010-7824(84)90097-0. [DOI] [PubMed] [Google Scholar]
  2. Asculai S. S., Weis M. T., Rancourt M. W., Kupferberg A. B. Inactivation of herpes simplex viruses by nonionic surfactants. Antimicrob Agents Chemother. 1978 Apr;13(4):686–690. doi: 10.1128/aac.13.4.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benes S., McCormack W. M. Inhibition of growth of Chlamydia trachomatis by nonoxynol-9 in vitro. Antimicrob Agents Chemother. 1985 May;27(5):724–726. doi: 10.1128/aac.27.5.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolch O. H., Jr, Warren J. C. In vitro effects of Emko on Neisseria gonorrhoeae and Trichomonas vaginalis. Am J Obstet Gynecol. 1973 Apr 15;115(8):1145–1148. doi: 10.1016/0002-9378(73)90566-8. [DOI] [PubMed] [Google Scholar]
  5. Chan K. Y., Patton D. L., Cosgrove Y. T. Time-lapse videomicroscopic study of in vitro wound closure in rabbit corneal cells. Invest Ophthalmol Vis Sci. 1989 Dec;30(12):2488–2498. [PubMed] [Google Scholar]
  6. Chan K. Y. Release of plasminogen activator by cultured corneal epithelial cells during differentiation and wound closure. Exp Eye Res. 1986 May;42(5):417–431. doi: 10.1016/0014-4835(86)90002-3. [DOI] [PubMed] [Google Scholar]
  7. Kelly J. P., Reynolds R. B., Stagno S., Louv W. C., Alexander W. J. In vitro activity of the spermicide nonoxynol-9 against Chlamydia trachomatis. Antimicrob Agents Chemother. 1985 May;27(5):760–762. doi: 10.1128/aac.27.5.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knight S. T., Lee S. H., Davis C. H., Moorman D. R., Hodinka R. L., Wyrick P. B. In vitro activity of nonoxynol-9 on McCoy cells infected with Chlamydia trachomatis. Sex Transm Dis. 1987 Jul-Sep;14(3):165–173. doi: 10.1097/00007435-198707000-00009. [DOI] [PubMed] [Google Scholar]
  9. Kuo C. C., Wang S. P., Holmes K. K., Grayston J. T. Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington. Infect Immun. 1983 Aug;41(2):865–868. doi: 10.1128/iai.41.2.865-868.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Singh B., Cutler J. C., Utidjian H. M. Studies on development of a vaginal preparation providing both prophylaxis against venereal disease, other genital infections and contraception. 3. In vitro effect of vaginal contraceptive and selected vaginal preparations of Candida albicans and Trichomonas vaginalis. Contraception. 1972 May;5(5):401–411. doi: 10.1016/0010-7824(72)90032-7. [DOI] [PubMed] [Google Scholar]
  11. Singh B., Cutler J. C., Utidjian H. M. Studies on the development of a vaginal preparation providing both prophylaxis against venereal disease and other genital infections and contraception. II. Effect in vitro of vaginal contraceptive and non-contraceptive preparations on Treponema pallidum and Neisseria gonorrhoeae. Br J Vener Dis. 1972 Feb;48(1):57–64. doi: 10.1136/sti.48.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Singh B., Posti B., Cutler J. C. Virucidal effect of certain chemical contraceptives on Type 2 herpesvirus. Am J Obstet Gynecol. 1976 Oct 15;126(4):422–425. doi: 10.1016/0002-9378(76)90630-x. [DOI] [PubMed] [Google Scholar]

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