Skip to main content
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1986 Feb;87(2):379–385. doi: 10.1111/j.1476-5381.1986.tb10827.x

Receptor-responses in fresh human ciliary muscle.

M D Lograno, A Reibaldi
PMCID: PMC1916554  PMID: 3006859

Abstract

The physiological and pharmacological properties of ciliary muscle isolated from fresh human eyes were investigated. The muscle exhibited no spontaneous activity. Concentration-dependent contractions in response to carbachol were competitively antagonized by atropine (pA2 = 8.95). The muscle, precontracted by carbachol (2.7 X 10(-4)M), responded to the application of isoprenaline by concentration-dependent relaxation blocked by propranolol (3.5 X 10(-9)M to 3.5 X 10(-8)M; pA2 = 9.15). Angiotensin-evoked contractions were antagonized by 8-Ala-angiotensin II (4.5 X 10(-8)M) in a competitive manner, but were not inhibited by phentolamine or propranolol. Contractions generated by electrical stimulation of the muscle (30 ms, 20 Hz, 60 pulses) were antagonized by atropine (10(-7) M) and tetrodotoxin (6.3 X 10(-7) M). Phentolamine and propranolol did not influence these responses. An increase of the external potassium concentration ([K+]o) from 5.4 to 158.8 mM produced a mechanical response, antagonized by atropine, but not influenced by tetrodotoxin, phentolamine or propranolol. The human ciliary muscle appears to carry muscarinic and angiotensin receptors and beta 2-adrenoceptors. The estimate of Katropine for muscarinic receptors mediating carbachol-induced contractions agrees with estimates of Katropine reported for human and rabbit iris.

Full text

PDF
379

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARUNLAKSHANA O., SCHILD H. O. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother. 1959 Mar;14(1):48–58. doi: 10.1111/j.1476-5381.1959.tb00928.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kaumann A. J., Hennekes R. The affinity of atropine for muscarine receptors in human sphincter pupillae. Naunyn Schmiedebergs Arch Pharmacol. 1979 Apr;306(3):209–211. doi: 10.1007/BF00507105. [DOI] [PubMed] [Google Scholar]
  3. Kuriyama H., Osa T., Toida N. Membrane properties of the smooth muscle of guinea-pig ureter. J Physiol. 1967 Jul;191(2):225–238. doi: 10.1113/jphysiol.1967.sp008247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nathanson J. A. Adrenergic regulation of intraocular pressure: identification of beta 2-adrenergic-stimulated adenylate cyclase in ciliary process epithelium. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7420–7424. doi: 10.1073/pnas.77.12.7420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. PATON W. D., RANG H. P. THE UPTAKE OF ATROPINE AND RELATED DRUGS BY INTESTINAL SMOOTH MUSCLE OF THE GUINEA-PIG IN RELATION TO ACETYLCHOLINE RECEPTORS. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:1–44. doi: 10.1098/rspb.1965.0058. [DOI] [PubMed] [Google Scholar]
  6. Reibaldi A., Lograno M. D., Avitabile T. Iris receptors of fresh human eyes. Ann Ophthalmol. 1984 Aug;16(8):746–748. [PubMed] [Google Scholar]
  7. Rowland J. M., Potter D. E. Effects of adrenergic drugs on aqueous cAMP and cGMP and intraocular pressure. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1979;212(2):65–75. doi: 10.1007/BF00587598. [DOI] [PubMed] [Google Scholar]
  8. Smith S. A. Factors determining the potency of mydriatic drugs in man. Br J Clin Pharmacol. 1976 Jun;3(3):503–507. doi: 10.1111/j.1365-2125.1976.tb00628.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Suzuki R. Neuronal influence on the mechanical activity of the ciliary muscle. Br J Pharmacol. 1983 Mar;78(3):591–597. doi: 10.1111/j.1476-5381.1983.tb08819.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thron C. D., Waud D. R. The rate of action of atropine. J Pharmacol Exp Ther. 1968 Mar;160(1):91–105. [PubMed] [Google Scholar]
  11. Turker R. K., Yamamoto M. Y., Khairallah P. A., Bumpus F. M. Competative antagonism of 8-ala-angiotensin II to angiotensins I and II on isolated rabbit aorta and rat colon. Eur J Pharmacol. 1971;15(3):285–291. doi: 10.1016/0014-2999(71)90094-x. [DOI] [PubMed] [Google Scholar]
  12. van Alphen G. W. The adrenergic receptors of the intraocular muscles of the human eye. Invest Ophthalmol. 1976 Jun;15(6):502–505. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES