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. 1985 Jun;85(2):557–563. doi: 10.1111/j.1476-5381.1985.tb08893.x

Adenosine-induced coronary release of prostacyclin at normal and low pH in isolated heart of rabbit.

G Ciabattoni, A Wennmalm
PMCID: PMC1916599  PMID: 3928011

Abstract

Rabbit hearts were perfused by the Langendorff method with drug-free perfusion medium or with a medium containing adenosine (10(-7) M-10(-4)M) and the coronary and transmyocardial efflux rates of 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) were measured. Perfusion was performed both at pH 7.4 and 6.9. In other experiments the hearts were pre-labelled with [14C]-arachidonic acid and the coronary efflux of radioactivity and of labelled lipids and 6-keto-PGF1 alpha were determined. The basal coronary flow was elevated by almost 70% during tissue acidosis, in comparison to control. Adenosine induced a dose-dependent increase in the coronary flow, amounting to about 75% at normal pH and a drug concentration of 10(-5)M. The adenosine-induced increase in coronary flow was not facilitated by low pH. The base coronary efflux of 6-keto-PGF1 alpha from the hearts was 2.5--3.6 ng min-1. Adenosine (10(-6)-10(-5)M) significantly facilitated this efflux, up to 6.5 ng min-1. The efflux of 6-keto-PGF1 alpha was not changed by perfusion with acidic medium, either in the basal state or during perfusion with adenosine. The basal interstitial efflux of 6-keto-PGF1 alpha was 4.5-5.5 ng 3 min-1. This efflux was not affected by perfusion of the heart with adenosine-containing medium. In hearts pre-labelled with [14C]-arachidonic acid, adenosine (10 microM) induced a specific liberation of labelled lipid-extractable substances, including 6-keto-PGF1 alpha.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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