Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1985 Oct;86(2):447–453. doi: 10.1111/j.1476-5381.1985.tb08914.x

Atrial natriuretic factor causes specific relaxation of rat renal arcuate arteries.

C Aalkjaer, M J Mulvany, N C Nyborg
PMCID: PMC1916701  PMID: 2932194

Abstract

We have investigated the effect of a synthetic 'atrial natriuretic factor' (ANF) on induced tone in rat isolated renal arcuate arteries (lumen diameter ca. 250 microns), and compared this with the effects of synthetic ANF on resistance vessels of similar size taken from the mesenteric, femoral, cerebral and coronary vasculature. Synthetic ANF was found to cause relaxation of the renal vessels when these were sub-maximally activated with K+, noradrenaline or 5-hydroxytryptamine, but had no effect on the responses of the other vessels to these agonists. Synthetic ANF had a near maximal effect (65% relaxation) at 100 nM, with an IC50 of 7.9 nM. The relaxant effect of synthetic ANF on the renal vessels was fully maintained for at least 15 min. Hydralazine (100 microM) caused relaxation of renal vessels (47%) and coronary vessels (42%), but had no effect on the other vessel types. By contrast, sodium nitroprusside (1 microM) relaxed all vessel types. The relaxant action of synthetic ANF on the renal vessels was seen in the presence of ouabain (1 mM), propranolol (1 microM), phentolamine (1 microM), atropine (1 microM) and felodipine (1 nM). In t renal vessels, synthetic ANF had no effect on membrane potential, measured with intracellular electrodes, despite the simultaneously measured relaxation. Synthetic ANF had no effect on the efflux of 22Na+ in either renal or mesenteric vessels. The results demonstrate that synthetic ANF has a specific and prolonged relaxant effect on renal small arteries, and are consistent with this effect being mediated through specific receptors.

Full text

PDF
447

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aalkjaer C., Mulvany M. J. Sodium metabolism in rat resistance vessels. J Physiol. 1983 Oct;343:105–116. doi: 10.1113/jphysiol.1983.sp014883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atarashi K., Mulrow P. J., Franco-Saenz R., Snajdar R., Rapp J. Inhibition of aldosterone production by an atrial extract. Science. 1984 Jun 1;224(4652):992–994. doi: 10.1126/science.6326267. [DOI] [PubMed] [Google Scholar]
  3. Borenstein H. B., Cupples W. A., Sonnenberg H., Veress A. T. The effect of a natriuretic atrial extract on renal haemodynamics and urinary excretion in anaesthetized rats. J Physiol. 1983 Jan;334:133–140. doi: 10.1113/jphysiol.1983.sp014484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Briggs J. P., Steipe B., Schubert G., Schnermann J. Micropuncture studies of the renal effects of atrial natriuretic substance. Pflugers Arch. 1982 Dec;395(4):271–276. doi: 10.1007/BF00580789. [DOI] [PubMed] [Google Scholar]
  5. Chartier L., Schiffrin E., Thibault G., Garcia R. Atrial natriuretic factor inhibits the stimulation of aldosterone secretion by angiotensin II, ACTH and potassium in vitro and angiotensin II-induced steroidogenesis in vivo. Endocrinology. 1984 Nov;115(5):2026–2028. doi: 10.1210/endo-115-5-2026. [DOI] [PubMed] [Google Scholar]
  6. Currie M. G., Geller D. M., Cole B. R., Siegel N. R., Fok K. F., Adams S. P., Eubanks S. R., Galluppi G. R., Needleman P. Purification and sequence analysis of bioactive atrial peptides (atriopeptins). Science. 1984 Jan 6;223(4631):67–69. doi: 10.1126/science.6419347. [DOI] [PubMed] [Google Scholar]
  7. De Léan A., Gutkowska J., McNicoll N., Schiller P. W., Cantin M., Genest J. Characterization of specific receptors for atrial natriuretic factor in bovine adrenal zona glomerulosa. Life Sci. 1984 Dec 3;35(23):2311–2318. doi: 10.1016/0024-3205(84)90522-8. [DOI] [PubMed] [Google Scholar]
  8. Hamet P., Tremblay J., Pang S. C., Garcia R., Thibault G., Gutkowska J., Cantin M., Genest J. Effect of native and synthetic atrial natriuretic factor on cyclic GMP. Biochem Biophys Res Commun. 1984 Sep 17;123(2):515–527. doi: 10.1016/0006-291x(84)90260-2. [DOI] [PubMed] [Google Scholar]
  9. Kangawa K., Matsuo H. Purification and complete amino acid sequence of alpha-human atrial natriuretic polypeptide (alpha-hANP). Biochem Biophys Res Commun. 1984 Jan 13;118(1):131–139. doi: 10.1016/0006-291x(84)91077-5. [DOI] [PubMed] [Google Scholar]
  10. Keeler R. Atrial natriuretic factor has a direct, prostaglandin-independent action on kidneys. Can J Physiol Pharmacol. 1982 Aug;60(8):1078–1082. doi: 10.1139/y82-155. [DOI] [PubMed] [Google Scholar]
  11. Khayyal M., Gross F., Kreye V. A. Studies on the direct vasodilator effect of hydralazine in the isolated rabbit renal artery. J Pharmacol Exp Ther. 1981 Feb;216(2):390–394. [PubMed] [Google Scholar]
  12. Kudo T., Baird A. Inhibition of aldosterone production in the adrenal glomerulosa by atrial natriuretic factor. Nature. 1984 Dec 20;312(5996):756–757. doi: 10.1038/312756a0. [DOI] [PubMed] [Google Scholar]
  13. Maack T., Marion D. N., Camargo M. J., Kleinert H. D., Laragh J. H., Vaughan E. D., Jr, Atlas S. A. Effects of auriculin (atrial natriuretic factor) on blood pressure, renal function, and the renin-aldosterone system in dogs. Am J Med. 1984 Dec;77(6):1069–1075. doi: 10.1016/0002-9343(84)90190-6. [DOI] [PubMed] [Google Scholar]
  14. Mulvany M. J., Halpern W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res. 1977 Jul;41(1):19–26. doi: 10.1161/01.res.41.1.19. [DOI] [PubMed] [Google Scholar]
  15. Mulvany M. J., Nilsson H., Flatman J. A. Role of membrane potential in the response of rat small mesenteric arteries to exogenous noradrenaline stimulation. J Physiol. 1982 Nov;332:363–373. doi: 10.1113/jphysiol.1982.sp014418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Napier M. A., Vandlen R. L., Albers-Schönberg G., Nutt R. F., Brady S., Lyle T., Winquist R., Faison E. P., Heinel L. A., Blaine E. H. Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5946–5950. doi: 10.1073/pnas.81.19.5946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nilsson H., Mulvany M. J. Prolonged exposure to ouabain eliminates the greater norepinephrine-dependent calcium sensitivity of resistance vessels in spontaneously hypertensive rats. Hypertension. 1981 Nov-Dec;3(6):691–697. doi: 10.1161/01.hyp.3.6.691. [DOI] [PubMed] [Google Scholar]
  18. Nyborg N. C., Mulvany M. J. Effect of felodipine, a new dihydropyridine vasodilator, on contractile responses to potassium, noradrenaline, and calcium in mesenteric resistance vessels of the rat. J Cardiovasc Pharmacol. 1984 May-Jun;6(3):499–505. doi: 10.1097/00005344-198405000-00019. [DOI] [PubMed] [Google Scholar]
  19. Oshima T., Currie M. G., Geller D. M., Needleman P. An atrial peptide is a potent renal vasodilator substance. Circ Res. 1984 May;54(5):612–616. doi: 10.1161/01.res.54.5.612. [DOI] [PubMed] [Google Scholar]
  20. Pamnani M. B., Clough D. L., Chen J. S., Link W. T., Haddy F. J. Effects of rat atrial extract on sodium transport and blood pressure in the rat. Proc Soc Exp Biol Med. 1984 Jun;176(2):123–131. doi: 10.3181/00379727-176-41851. [DOI] [PubMed] [Google Scholar]
  21. Seidman C. E., Duby A. D., Choi E., Graham R. M., Haber E., Homcy C., Smith J. A., Seidman J. G. The structure of rat preproatrial natriuretic factor as defined by a complementary DNA clone. Science. 1984 Jul 20;225(4659):324–326. doi: 10.1126/science.6234658. [DOI] [PubMed] [Google Scholar]
  22. Sonnenberg H., Cupples W. A., de Bold A. J., Veress A. T. Intrarenal localization of the natriuretic effect of cardiac atrial extract. Can J Physiol Pharmacol. 1982 Sep;60(9):1149–1152. doi: 10.1139/y82-166. [DOI] [PubMed] [Google Scholar]
  23. Thibault G., Garcia R., Cantin M., Genest J., Lazure C., Seidah N. G., Chrétien M. Primary structure of a high Mr form of rat atrial natriuretic factor. FEBS Lett. 1984 Feb 27;167(2):352–356. doi: 10.1016/0014-5793(84)80155-6. [DOI] [PubMed] [Google Scholar]
  24. Winquist R. J., Faison E. P., Nutt R. F. Vasodilator profile of synthetic atrial natriuretic factor. Eur J Pharmacol. 1984 Jun 15;102(1):169–173. doi: 10.1016/0014-2999(84)90353-4. [DOI] [PubMed] [Google Scholar]
  25. Winquist R. J., Faison E. P., Waldman S. A., Schwartz K., Murad F., Rapoport R. M. Atrial natriuretic factor elicits an endothelium-independent relaxation and activates particulate guanylate cyclase in vascular smooth muscle. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7661–7664. doi: 10.1073/pnas.81.23.7661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. de Bold A. J., Borenstein H. B., Veress A. T., Sonnenberg H. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci. 1981 Jan 5;28(1):89–94. doi: 10.1016/0024-3205(81)90370-2. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES