Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1986 Sep;89(1):27–33. doi: 10.1111/j.1476-5381.1986.tb11117.x

Tetrodotoxin-sensitive, K+-induced relaxation of guinea-pig isolated trachealis in the presence of Ca2+-entry blocking drugs, Ca2+-free solution and after polyamine exposure.

E W Chideckel, J S Fedan, P Mike
PMCID: PMC1917042  PMID: 3801775

Abstract

We have previously observed a paradoxical relaxant effect of K+ on guinea-pig isolated trachealis after exposure to polyamines. The purpose of the present study was to evaluate whether the relaxation involved a reduction in the entry of extracellular Ca2+. We therefore investigated the effect of K+ in the presence of Ca2+-entry blocking drugs and in the presence of Ca2+-free solution. In the presence of nifedipine (10(-5) M), verapamil (10(-5) M) or diltiazem (10(-5) M), K+ (30 mM) induced relaxation of the trachealis muscle. The relaxation to K+ was not blocked by ouabain (10(-6) M), propranolol (10(-6) M), or indomethacin (10(-6) M). A relaxation in response to K+ was also observed in Ca2+-free solution, (with tone induced by methacholine), an effect not blocked by propranolol or ouabain. Tetraethylammonium (30 mM) (TEA), which ordinarily evokes contractile responses, induced trachealis relaxation in the presence of verapamil or nifedipine. The relaxation was unaltered by ouabain or propranolol. Tetrodotoxin (10(-6) M) (TTX) blocked 65% of the K+-induced relaxation in the presence of nifedipine and 100% of K+-induced relaxation either in a Ca2+-free solution or after polyamine exposure. TTX was without effect on TEA-induced relaxation after Ca2+-entry blocking drugs. Atropine (10(-6) M) or hexamethonium (10(-6) M) did not affect K+-induced relaxation after polyamine exposure. The concentration-response curve for K+-induced contraction in normal modified Krebs-Henseleit solution was shifted to the left by TTX.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
27

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURNSTOCK G., CAMPBELL G., BENNETT M., HOLMAN M. E. INNERVATION OF THE GUINEA-PIG TAENIA COLI: ARE THERE INTRINSIC INHIBITORY NERVES WHICH ARE DISTINCT FROM SYMPATHETIC NERVES? Int J Neuropharmacol. 1964 May;3:163–166. doi: 10.1016/0028-3908(64)90003-6. [DOI] [PubMed] [Google Scholar]
  2. Chideckel E. W., Fedan J. S., Mike P. Polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig. Eur J Pharmacol. 1985 Oct 8;116(1-2):187–190. doi: 10.1016/0014-2999(85)90203-1. [DOI] [PubMed] [Google Scholar]
  3. Coburn R. F., Tomita T. Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle. Am J Physiol. 1973 May;224(5):1072–1080. doi: 10.1152/ajplegacy.1973.224.5.1072. [DOI] [PubMed] [Google Scholar]
  4. De Meis L. Relaxing effect of spermine and spermidine on intact and glycerol-treated muscle. Am J Physiol. 1967 Jan;212(1):92–96. doi: 10.1152/ajplegacy.1967.212.1.92. [DOI] [PubMed] [Google Scholar]
  5. Foster R. W., Okpalugo B. I., Small R. C. Antagonism of Ca2+ and other actions of verapamil in guinea-pig isolated trachealis. Br J Pharmacol. 1984 Mar;81(3):499–507. doi: 10.1111/j.1476-5381.1984.tb10103.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Foster R. W., Small R. C., Weston A. H. Evidence that the spasmogenic action of tetraethylammonium in guinea-pig trachealis is both direct and dependent on the cellular influx of calcium ion. Br J Pharmacol. 1983 May;79(1):255–263. doi: 10.1111/j.1476-5381.1983.tb10519.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Foster R. W., Small R. C., Weston A. H. The spasmogenic action of potassium chloride in guinea-pig trachealis. Br J Pharmacol. 1983 Nov;80(3):553–559. doi: 10.1111/j.1476-5381.1983.tb10728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hashimoto H., Unemoto T., Hayashi M. Inhibitory action of spermine on the contractions of rat uterus. Am J Physiol. 1973 Sep;225(3):743–746. doi: 10.1152/ajplegacy.1973.225.3.743. [DOI] [PubMed] [Google Scholar]
  9. Hay D. W., Wadsworth R. M. Effects of KCl on 45Ca uptake and efflux in the rat vas deferens. Br J Pharmacol. 1984 Mar;81(3):441–447. doi: 10.1111/j.1476-5381.1984.tb10096.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirkpatrick C. T. Excitation and contraction in bovine tracheal smooth muscle. J Physiol. 1975 Jan;244(2):263–281. doi: 10.1113/jphysiol.1975.sp010796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koenig H., Goldstone A., Lu C. Y. Polyamines regulate calcium fluxes in a rapid plasma membrane response. Nature. 1983 Oct 6;305(5934):530–534. doi: 10.1038/305530a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES