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. 1986 May;88(1):33–39. doi: 10.1111/j.1476-5381.1986.tb09468.x

Effects of Bay K 8644 on contraction of the human isolated bronchus and guinea-pig isolated trachea.

C Advenier, E Naline, A Renier
PMCID: PMC1917117  PMID: 2423176

Abstract

The effects of Bay K 8644, a dihydropyridine which increases calcium flux through the potential-operated channels were studied on the contractions induced by histamine, acetylcholine, KCl and Ca2+ on human isolated bronchial strips and the results were compared to those obtained on guinea-pig isolated tracheal spirals. Subsequently the contractant effects of Bay K 8644 in K+-enriched medium and in the presence of Ca2+ 0.03 mM were investigated. In Krebs normal calcium medium, Bay K 8644 did not significantly modify the EC50 of acetylcholine or histamine on the human bronchus, but in concentrations of 10(-7)-10(-6)M it potentiated the effects of KCl on that preparation. It did not modify the EC50 of acetylcholine, histamine or KCl on the guinea-pig trachea. In Ca2+-free Krebs medium with additional K+ (30 mM), Ca2+ concentration-response curves were displaced to the left by Bay K 8644 in the two preparations. Shifts were 0.52 +/- 0.11 and 0.72 +/- 0.16 log units respectively with Bay K 8644 10(-8) and 10(-7) M on human bronchus (n = 4) and 0.67 +/- 0.16 and 1.06 +/- 0.19 log units respectively with Bay K 8644 10(-7) and 10(-6) M on the guinea-pig trachea (n = 5). In Krebs medium with Ca2+ 0.03 mM and K+ 30 mM, Bay K 8644 (10(-8) to 10(-6) M) contracted both the human bronchus and the guinea-pig isolated trachea. This effect was competitively antagonized by nicardipine.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. ARUNLAKSHANA O., SCHILD H. O. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother. 1959 Mar;14(1):48–58. doi: 10.1111/j.1476-5381.1959.tb00928.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Advenier C., Cerrina J., Duroux P., Floch A., Renier A. Effects of five different organic calcium antagonists on guinea-pig isolated trachea. Br J Pharmacol. 1984 Jul;82(3):727–733. doi: 10.1111/j.1476-5381.1984.tb10812.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen S. L., Foster R. W., Small R. C., Towart R. The effects of the dihydropyridine Bay K 8644 in guinea-pig isolated trachealis. Br J Pharmacol. 1985 Sep;86(1):171–180. doi: 10.1111/j.1476-5381.1985.tb09447.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brink C., Grimaud C., Guillot C., Orehek J. The interaction between indomethacin and contractile agents on human isolated airway muscle. Br J Pharmacol. 1980 Jul;69(3):383–388. doi: 10.1111/j.1476-5381.1980.tb07026.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cerrina J., Advenier C., Renier A., Floch A., Duroux P. Effects of diltiazem and other Ca2+ antagonists on guinea-pig tracheal muscle. Eur J Pharmacol. 1983 Oct 28;94(3-4):241–249. doi: 10.1016/0014-2999(83)90413-2. [DOI] [PubMed] [Google Scholar]
  6. Finet M., Godfraind T., Khoury G. The positive inotropic action of the nifedipine analogue, Bay K 8644, in guinea-pig and rat isolated cardiac preparations. Br J Pharmacol. 1985 Sep;86(1):27–32. doi: 10.1111/j.1476-5381.1985.tb09431.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Foster R. W., Small R. C., Weston A. H. Evidence that the spasmogenic action of tetraethylammonium in guinea-pig trachealis is both direct and dependent on the cellular influx of calcium ion. Br J Pharmacol. 1983 May;79(1):255–263. doi: 10.1111/j.1476-5381.1983.tb10519.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foster R. W., Small R. C., Weston A. H. The spasmogenic action of potassium chloride in guinea-pig trachealis. Br J Pharmacol. 1983 Nov;80(3):553–559. doi: 10.1111/j.1476-5381.1983.tb10728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ghelani A. M., Holroyde M. C., Sheard P. Response of human isolated bronchial and lung parenchymal strips to SRS-A and other mediators of asthmatic bronchospasm. Br J Pharmacol. 1980;71(1):107–112. doi: 10.1111/j.1476-5381.1980.tb10915.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Godfraind T., Kaba A., Polster P. Differences in sensitivity of arterial smooth muscles to inhibition of their contractile response to depolarization by potassium. Arch Int Pharmacodyn Ther. 1968 Mar;172(1):235–239. [PubMed] [Google Scholar]
  11. Guillot C., Fornaris M., Badier M., Orehek J. Spontaneous and provoked resistance to isoproterenol in isolated human bronchi. J Allergy Clin Immunol. 1984 Nov;74(5):713–718. doi: 10.1016/0091-6749(84)90235-5. [DOI] [PubMed] [Google Scholar]
  12. Kanmura Y., Itoh T., Kuriyama H. Agonist actions of Bay K 8644, a dihydropyridine derivative, on the voltage-dependent calcium influx in smooth muscle cells of the rabbit mesenteric artery. J Pharmacol Exp Ther. 1984 Dec;231(3):717–723. [PubMed] [Google Scholar]
  13. Schramm M., Thomas G., Towart R., Franckowiak G. Novel dihydropyridines with positive inotropic action through activation of Ca2+ channels. Nature. 1983 Jun 9;303(5917):535–537. doi: 10.1038/303535a0. [DOI] [PubMed] [Google Scholar]
  14. Spedding M., Berg C. Interactions between a "calcium channel agonist", Bay K 8644, and calcium antagonists differentiate calcium antagonist subgroups in K+-depolarized smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 1984 Nov;328(1):69–75. doi: 10.1007/BF00496109. [DOI] [PubMed] [Google Scholar]
  15. Spedding M. Competitive interactions between Bay K 8644 and nifedipine in K+ depolarized smooth muscle: a passive role for Ca2+? Naunyn Schmiedebergs Arch Pharmacol. 1985 Feb;328(4):464–466. doi: 10.1007/BF00692917. [DOI] [PubMed] [Google Scholar]
  16. Vaghy P. L., Grupp I. L., Grupp G., Balwierczak J. L., Williams J. S., Schwartz A. Correlation of nitrendipine and Bay k 8644 binding to isolated canine heart sarcolemma with their pharmacological effects on the canine heart. Eur J Pharmacol. 1984 Jul 13;102(2):373–374. doi: 10.1016/0014-2999(84)90273-5. [DOI] [PubMed] [Google Scholar]
  17. Vincenc K., Black J., Shaw J. Relaxation and contraction responses to histamine in the human lung parenchymal strip. Eur J Pharmacol. 1984 Feb 17;98(2):201–210. doi: 10.1016/0014-2999(84)90591-0. [DOI] [PubMed] [Google Scholar]
  18. Yamamoto H., Hwang O., Van Breemen C. Bay K8644 differentiates between potential and receptor operated Ca2+ channels. Eur J Pharmacol. 1984 Jul 20;102(3-4):555–557. doi: 10.1016/0014-2999(84)90581-8. [DOI] [PubMed] [Google Scholar]

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