Abstract
An intravenous infusion of platelet activating factor (Paf) in the guinea-pig elicits an increase in bronchial responsiveness to the spasmogens, histamine and bombesin. Airways obstruction induced by bombesin in Paf-treated animals is poorly reversed by isoprenaline compared to comparable airways obstruction induced by bombesin in vehicle-treated animals. Isoprenaline induced a comparable dose-related relaxation in vitro of tracheal smooth muscle isolated from Paf- and vehicle-treated animals. No change in beta-adrenoceptor numbers or binding affinity was observed in lungs removed from Paf-treated animals in comparison with those from vehicle-treated animals, or after direct incubation with Paf in vitro. The reduced bronchodilator responsiveness to isoprenaline in Paf-treated animals is not related to changes in pulmonary beta-adrenoceptor function. These results suggest that non-spasmogenic elements may contribute to airways obstruction induced in hyper-responsive animals.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnes P., Karliner J., Hamilton C., Dollery C. Demonstration of alpha 1-adrenoceptors in guinea pig lung using 3H-prazosin. Life Sci. 1979 Oct 1;25(14):1207–1214. doi: 10.1016/0024-3205(79)90462-4. [DOI] [PubMed] [Google Scholar]
- Carswell H., Nahorski S. R. Beta-adrenoceptor heterogeneity in guinea-pig airways: comparison of functional and receptor labelling studies. Br J Pharmacol. 1983 Aug;79(4):965–971. doi: 10.1111/j.1476-5381.1983.tb10542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chung K. F., Aizawa H., Leikauf G. D., Ueki I. F., Evans T. W., Nadel J. A. Airway hyperresponsiveness induced by platelet-activating factor: role of thromboxane generation. J Pharmacol Exp Ther. 1986 Mar;236(3):580–584. [PubMed] [Google Scholar]
- Cockcroft D. W. Mechanism of perennial allergic asthma. Lancet. 1983 Jul 30;2(8344):253–256. doi: 10.1016/s0140-6736(83)90235-0. [DOI] [PubMed] [Google Scholar]
- Cuss F. M., Dixon C. M., Barnes P. J. Effects of inhaled platelet activating factor on pulmonary function and bronchial responsiveness in man. Lancet. 1986 Jul 26;2(8500):189–192. doi: 10.1016/s0140-6736(86)92489-x. [DOI] [PubMed] [Google Scholar]
- Fabbri L. M. Airway inflammation and asthma. Importance of arachidonate metabolites for airway hyperresponsiveness. Prog Biochem Pharmacol. 1985;20:18–25. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lellouch-Tubiana A., Lefort J., Pirotzky E., Vargaftig B. B., Pfister A. Ultrastructural evidence for extravascular platelet recruitment in the lung upon intravenous injection of platelet-activating factor (PAF-acether) to guinea-pigs. Br J Exp Pathol. 1985 Jun;66(3):345–355. [PMC free article] [PubMed] [Google Scholar]
- Metzger W. J., Hunninghake G. W., Richerson H. B. Late asthmatic responses: inquiry into mechanisms and significance. Clin Rev Allergy. 1985 May;3(2):145–165. doi: 10.1007/BF02992980. [DOI] [PubMed] [Google Scholar]
- Morley J., Sanjar S., Page C. P. The platelet in asthma. Lancet. 1984 Nov 17;2(8412):1142–1144. doi: 10.1016/s0140-6736(84)91567-8. [DOI] [PubMed] [Google Scholar]
- Patterson R., Harris K. E. The activity of aerosolized and intracutaneous synthetic platelet activating factor (AGEPC) in rhesus monkeys with IgE-mediated airway responses and normal monkeys. J Lab Clin Med. 1983 Dec;102(6):933–938. [PubMed] [Google Scholar]
- Tiribelli C., Lunazzi G. C., Sottocasa G. L. Mechanisms of hepatic uptake of organic anions. Clin Sci (Lond) 1986 Jul;71(1):1–8. doi: 10.1042/cs0710001. [DOI] [PubMed] [Google Scholar]
- Wallenstein S., Zucker C. L., Fleiss J. L. Some statistical methods useful in circulation research. Circ Res. 1980 Jul;47(1):1–9. doi: 10.1161/01.res.47.1.1. [DOI] [PubMed] [Google Scholar]