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. 1987 Jan;90(1):3–5. doi: 10.1111/j.1476-5381.1987.tb16818.x

Muscarinic receptors mediating suppression of the M-current in guinea-pig olfactory cortex neurones may be of the M2-subtype.

A Constanti, J A Sim
PMCID: PMC1917286  PMID: 3814922

Abstract

Guinea-pig olfactory cortical neurones in vitro were voltage clamped by means of a single intracellular microelectrode technique. Hyperpolarizing voltage commands from holding potentials between -40 to -50 mV produced slow inward current relaxations reflecting deactivation of the M-current (IM). IM was reversibly suppressed by 30 microM muscarine or carbachol; this suppression was insensitive to pirenzepine (up to 300 nM) but was inhibited by gallamine (10-20 microM) or 4-diphenyl-acetoxy-N-methylpiperidine (100, 500 nM), suggesting the involvement of the M2-type muscarinic receptor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barlow R. B., Berry K. J., Glenton P. A., Nilolaou N. M., Soh K. S. A comparison of affinity constants for muscarine-sensitive acetylcholine receptors in guinea-pig atrial pacemaker cells at 29 degrees C and in ileum at 29 degrees C and 37 degrees C. Br J Pharmacol. 1976 Dec;58(4):613–620. doi: 10.1111/j.1476-5381.1976.tb08631.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown D. A., Forward A., Marsh S. Antagonist discrimination between ganglionic and ileal muscarinic receptors. Br J Pharmacol. 1980;71(2):362–364. doi: 10.1111/j.1476-5381.1980.tb10948.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Constanti A., Galvan M. Fast inward-rectifying current accounts for anomalous rectification in olfactory cortex neurones. J Physiol. 1983 Feb;335:153–178. doi: 10.1113/jphysiol.1983.sp014526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Constanti A., Galvan M. M-current in voltage-clamped olfactory cortex neurones. Neurosci Lett. 1983 Aug 19;39(1):65–70. doi: 10.1016/0304-3940(83)90166-0. [DOI] [PubMed] [Google Scholar]
  5. Egan T. M., North R. A. Acetylcholine acts on m2-muscarinic receptors to excite rat locus coeruleus neurones. Br J Pharmacol. 1985 Aug;85(4):733–735. doi: 10.1111/j.1476-5381.1985.tb11070.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Egan T. M., North R. A. Acetylcholine hyperpolarizes central neurones by acting on an M2 muscarinic receptor. 1986 Jan 30-Feb 5Nature. 319(6052):405–407. doi: 10.1038/319405a0. [DOI] [PubMed] [Google Scholar]
  7. Hammer R., Berrie C. P., Birdsall N. J., Burgen A. S., Hulme E. C. Pirenzepine distinguishes between different subclasses of muscarinic receptors. Nature. 1980 Jan 3;283(5742):90–92. doi: 10.1038/283090a0. [DOI] [PubMed] [Google Scholar]
  8. Hammer R., Giachetti A. Muscarinic receptor subtypes: M1 and M2 biochemical and functional characterization. Life Sci. 1982 Dec 27;31(26):2991–2998. doi: 10.1016/0024-3205(82)90066-2. [DOI] [PubMed] [Google Scholar]
  9. McCormick D. A., Prince D. A. Acetylcholine induces burst firing in thalamic reticular neurones by activating a potassium conductance. 1986 Jan 30-Feb 5Nature. 319(6052):402–405. doi: 10.1038/319402a0. [DOI] [PubMed] [Google Scholar]
  10. Nedoma J., Dorofeeva N. A., Tucek S., Shelkovnikov S. A., Danilov A. F. Interaction of the neuromuscular blocking drugs alcuronium, decamethonium, gallamine, pancuronium, ritebronium, tercuronium and d-tubocurarine with muscarinic acetylcholine receptors in the heart and ileum. Naunyn Schmiedebergs Arch Pharmacol. 1985 Apr;329(2):176–181. doi: 10.1007/BF00501209. [DOI] [PubMed] [Google Scholar]

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