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. 1990 Feb;99(2):427–433. doi: 10.1111/j.1476-5381.1990.tb14720.x

Simultaneous perfusion of rat isolated superior mesenteric arterial and venous beds: comparison of their vasoconstrictor and vasodilator responses to agonists.

T D Warner 1
PMCID: PMC1917370  PMID: 2328405

Abstract

1. A new isolated perfused preparation is described that allows a direct comparison to be made of the responses of the perfused arterial and retrogradely perfused venous circulations of the rat superior mesenteric vascular bed. 2. In experiments comparing the responses of the intact arterially perfused mesentery and small intestine to those of the same preparation following removal of the intestine and division of the circulations, the increases in perfusion pressure produced by arginine-vasopressin (30 pmol) and noradrenaline (1 nmol) were retained by the arterial circulation and those induced by angiotensin II (30 pmol) by the venous circulation. Endothelin-1 (30 pmol) constricted both portions of the vasculature but the prolonged nature of its response was associated with only the venous vessels. 3. In the simultaneously perfused arterial and venous preparation arginine vasopressin (3-100 pmol) was a selective constrictor of the arterial circulation and angiotensin II (3-100 pmol) of the venous circulation. In addition, noradrenaline (0.3-10 nmol), 5-hydroxytryptamine (0.3-10 nmol) and KCl (1-60 micromol) were more active as constrictors of the arterial than the venous vessels, and U46619 (10-300 pmol) a more active constrictor of the venous than the arterial vessels. Endothelin-1 (3-100 pmol) constricted both the arterial and venous portions of the vasculature but was significantly longer acting as a venoconstrictor than an arterioconstrictor. 4. Angiotensin I (300 pmol) caused constrictions of the venous circulation which were dependent upon the presence of angiotensin converting enzyme for captopril (10 microM) abolished constrictions caused by angiotensin I but not by angiotensin II.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. ABLAD B., MELLANDER S. COMPARATIVE EFFECTS OF HYDRALAZINE, SODIUM NITRITE AND ACETYLCHOLINE ON RESISTANCE AND CAPACITANCE BLOOD VESSELS AND CAPILLARY FILTRATION IN SKELETAL MUSCLE IN THE CAT. Acta Physiol Scand. 1963 Aug;58:319–329. doi: 10.1111/j.1748-1716.1963.tb02654.x. [DOI] [PubMed] [Google Scholar]
  2. Bhardwaj R., Moore P. K. Endothelium-derived relaxing factor and the effects of acetylcholine and histamine on resistance blood vessels. Br J Pharmacol. 1988 Nov;95(3):835–843. doi: 10.1111/j.1476-5381.1988.tb11712.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bohlen H. G., Gore R. W. Comparison of microvascular pressures and diameters in the innervated and denervated rat intestine. Microvasc Res. 1977 Nov;14(3):251–264. doi: 10.1016/0026-2862(77)90024-3. [DOI] [PubMed] [Google Scholar]
  4. Cherry P. D., Furchgott R. F., Zawadzki J. V., Jothianandan D. Role of endothelial cells in relaxation of isolated arteries by bradykinin. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2106–2110. doi: 10.1073/pnas.79.6.2106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Mey J. G., Vanhoutte P. M. Heterogeneous behavior of the canine arterial and venous wall. Importance of the endothelium. Circ Res. 1982 Oct;51(4):439–447. doi: 10.1161/01.res.51.4.439. [DOI] [PubMed] [Google Scholar]
  6. Dresel P., Wallentin I. Effects of sympathetic vasoconstrictor fibres, noradrenaline and vasopressin on the intestinal vascular resistance during constant blood flow or blood pressure. Acta Physiol Scand. 1966 Apr;66(4):427–436. doi: 10.1111/j.1748-1716.1966.tb03220.x. [DOI] [PubMed] [Google Scholar]
  7. Emerson T. E., Jr Changes of venous return and other hemodynamic parameters during bradykinin infusion. Am J Physiol. 1967 Jun;212(6):1455–1460. doi: 10.1152/ajplegacy.1967.212.6.1455. [DOI] [PubMed] [Google Scholar]
  8. Emerson T. E., Jr Effects of acetylcholine, histamine, and serotonin infusion on venous return in dogs. Am J Physiol. 1968 Jul;215(1):41–48. doi: 10.1152/ajplegacy.1968.215.1.41. [DOI] [PubMed] [Google Scholar]
  9. Emerson T. E., Jr Effects of angiotensin, epinephrine, norepinephrine, and vasopressin on venous return. Am J Physiol. 1966 May;210(5):933–942. doi: 10.1152/ajplegacy.1966.210.5.933. [DOI] [PubMed] [Google Scholar]
  10. FOLKOW B., LEWIS D. H., LUNDGREN O., MELLANDER S., WALLENTIN I. THE EFFECT OF GRADED VASOCONSTRICTOR FIBRE STIMULATION ON THE INTESTINAL RESISTANCE AND CAPACITANCE VESSELS. Acta Physiol Scand. 1964 Aug;61:445–457. [PubMed] [Google Scholar]
  11. FOLKOW B., LUNDGREN O., WALLENTIN I. Studies on the relationship between flow resistance, capillary filtration coefficient and regional blood volume in the intestine of the cat. Acta Physiol Scand. 1963 Mar;57:270–283. doi: 10.1111/j.1748-1716.1963.tb02591.x. [DOI] [PubMed] [Google Scholar]
  12. FOLKOW B., MELLANDER S. VEINS AND VENOUS TONE. Am Heart J. 1964 Sep;68:397–408. doi: 10.1016/0002-8703(64)90308-4. [DOI] [PubMed] [Google Scholar]
  13. Fortes Z. B., de Nucci G., Garcia-Leme J. Effect of endothelin-1 on arterioles and venules in vivo. J Cardiovasc Pharmacol. 1989;13 (Suppl 5):S200–S201. doi: 10.1097/00005344-198900135-00056. [DOI] [PubMed] [Google Scholar]
  14. Furchgott R. F., Carvalho M. H., Khan M. T., Matsunaga K. Evidence for endothelium-dependent vasodilation of resistance vessels by acetylcholine. Blood Vessels. 1987;24(3):145–149. doi: 10.1159/000158689. [DOI] [PubMed] [Google Scholar]
  15. Förstermann U., Goppelt-Strübe M., Frölich J. C., Busse R. Inhibitors of acyl-coenzyme A:lysolecithin acyltransferase activate the production of endothelium-derived vascular relaxing factor. J Pharmacol Exp Ther. 1986 Jul;238(1):352–359. [PubMed] [Google Scholar]
  16. Gaehtgens P., Uekermann U. The distensibility of mesenteric venous microvessels. Pflugers Arch. 1971;330(3):206–216. doi: 10.1007/BF00588612. [DOI] [PubMed] [Google Scholar]
  17. Gore R. W. Wall stress: a determinant of regional differences in response of frog microvessels to norepinephrine. Am J Physiol. 1972 Jan;222(1):82–91. doi: 10.1152/ajplegacy.1972.222.1.82. [DOI] [PubMed] [Google Scholar]
  18. Henrich H. A. Precapillary control of the splanchnic vascular bed. J Cardiovasc Pharmacol. 1985;7 (Suppl 3):S73–S79. doi: 10.1097/00005344-198500073-00009. [DOI] [PubMed] [Google Scholar]
  19. Hiley C. R., Douglas S. A., Randall M. D. Pressor effects of endothelin-1 and some analogs in the perfused superior mesenteric arterial bed of the rat. J Cardiovasc Pharmacol. 1989;13 (Suppl 5):S197–S199. doi: 10.1097/00005344-198900135-00055. [DOI] [PubMed] [Google Scholar]
  20. Hébert M. T., Marshall J. M. Direct observations of responses of mesenteric microcirculation of the rat to circulating noradrenaline. J Physiol. 1985 Nov;368:393–407. doi: 10.1113/jphysiol.1985.sp015864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MCGREGOR D. D. THE EFFECT OF SYMPATHETIC NERVE STIMULATION OF VASOCONSTRICTOR RESPONSES IN PERFUSED MESENTERIC BLOOD VESSELS OF THE RAT. J Physiol. 1965 Mar;177:21–30. doi: 10.1113/jphysiol.1965.sp007572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. MELLANDER S. Comparative studies on the adrenergic neuro-hormonal control of resistance and capacitance blood vessels in the cat. Acta Physiol Scand Suppl. 1960;50(176):1–86. [PubMed] [Google Scholar]
  23. Oberg B. The relationship between active constriction and passive recoil of the veins at various distending pressures. Acta Physiol Scand. 1967 Oct-Nov;71(2):233–247. doi: 10.1111/j.1748-1716.1967.tb03729.x. [DOI] [PubMed] [Google Scholar]
  24. Ralevic V., Burnstock G. Actions mediated by P2-purinoceptor subtypes in the isolated perfused mesenteric bed of the rat. Br J Pharmacol. 1988 Oct;95(2):637–645. doi: 10.1111/j.1476-5381.1988.tb11686.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Randall M. D., Kay A. P., Hiley C. R. Endothelium-dependent modulation of the pressor activity of arginine vasopressin in the isolated superior mesenteric arterial bed of the rat. Br J Pharmacol. 1988 Oct;95(2):646–652. doi: 10.1111/j.1476-5381.1988.tb11687.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Richardson D. R., Zweifach B. W. Pressure relationships in the macro- and microcirculation of the mesentery. Microvasc Res. 1970 Oct;2(4):474–488. doi: 10.1016/0026-2862(70)90040-3. [DOI] [PubMed] [Google Scholar]
  27. SELKURT E. E., JOHNSON P. C. Effect of acute elevation of portal venous pressure on mesenteric blood volume, interstitial fluid volume and hemodynamics. Circ Res. 1958 Sep;6(5):592–599. doi: 10.1161/01.res.6.5.592. [DOI] [PubMed] [Google Scholar]
  28. Singbartl G., Henrich H. Adrenergic-induced vascular adjustments--initial and escape reactions. II. Role of beta-adrenergic receptors within different sections of the isolated intestinal vascular bed. Pflugers Arch. 1974 Jan 16;346(1):13–24. doi: 10.1007/BF00592646. [DOI] [PubMed] [Google Scholar]
  29. Toda N., Bian K., Akiba T., Okamura T. Heterogeneity in mechanisms of bradykinin action in canine isolated blood vessels. Eur J Pharmacol. 1987 Mar 31;135(3):321–329. doi: 10.1016/0014-2999(87)90681-9. [DOI] [PubMed] [Google Scholar]
  30. Vedernikov Y. P., Gräser T., Tiedt N., Vikhert A. M. Heterogeneity of the response of venous smooth muscle to arterial endothelium-derived relaxing factor (EDRF) in respect of the role of nitric oxide. Basic Res Cardiol. 1988 Mar-Apr;83(2):122–127. doi: 10.1007/BF01907265. [DOI] [PubMed] [Google Scholar]
  31. Walder C. E., Thomas G. R., Thiemermann C., Vane J. R. The hemodynamic effects of endothelin-1 in the pithed rat. J Cardiovasc Pharmacol. 1989;13 (Suppl 5):S93–S102. doi: 10.1097/00005344-198900135-00023. [DOI] [PubMed] [Google Scholar]
  32. de Nucci G., Thomas R., D'Orleans-Juste P., Antunes E., Walder C., Warner T. D., Vane J. R. Pressor effects of circulating endothelin are limited by its removal in the pulmonary circulation and by the release of prostacyclin and endothelium-derived relaxing factor. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9797–9800. doi: 10.1073/pnas.85.24.9797. [DOI] [PMC free article] [PubMed] [Google Scholar]

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