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. 1990 Jun;100(2):348–352. doi: 10.1111/j.1476-5381.1990.tb15807.x

No effect of pertussis toxin on peripheral prejunctional alpha 2-adrenoceptor-mediated responses and on endothelium-dependent relaxations in the rat.

J R Docherty 1
PMCID: PMC1917421  PMID: 2165838

Abstract

1. We have investigated the effects of pertussis toxin treatment on a variety of peripheral tissues in the rat. 2. Incubation with pertussis toxin (1 microgram ml-1) in vitro failed to alter the negative inotropic actions of acetylcholine in rat left atria. 3. Pretreatment with pertussis toxin (6 micrograms kg-1, i.v., 3-4 days) abolished the negative inotropic actions of acetylcholine in rat left atria. 4. Pretreatment with pertussis toxin (40 micrograms kg-1, i.v., 3-4 days) failed to alter the prejunctional inhibitory actions of the alpha 2-adrenoceptor agonist xylazine, either in terms of the isometric contraction to a single stimulus in rat vas deferens or in terms of stimulation-evoked overflow of tritium in atria pre-incubated with [3H]-noradrenaline. 5. Pretreatment with pertussis toxin (6 micrograms kg-1, i.v., 3-4 days) failed to affect, and pertussis toxin (40 micrograms kg-1, i.v., 3-4 days) potentiated endothelium-dependent relaxations of rat aorta to histamine and acetylcholine. 6. It seems unlikely that peripheral prejunctional actions of alpha 2-adrenoceptor agonists or endothelium-dependent relaxations of rat aorta involve pertussis toxin-sensitive G proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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